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CYP2A6 and CYP2E1 immunoreactivities in bronchial epithelial cells affected by smoking in non-small cell lung cancer patients.

Research Project

Project/Area Number 14570312
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

OYAMA Tsunehiro  University of Occupational and Environmental Health, Departments of Environmental Health, Associate professor, 医学部, 助教授 (00309965)

Co-Investigator(Kenkyū-buntansha) TOYOHI Isse  University of Occupational and Environmental Health, Departments of Environmental Health, assistant professor, 医学部, 助手 (80341494)
KAWAMOTO Toshihiro  University of Occupational and Environmental Health, Departments of Environmental Health, professor, 医学部, 教授 (60177748)
松本 明子  佐賀医科大学, 医学部, 助手 (10330979)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsCytochromeP450 2A6 / CytochromeP450 2E1 / p53 / Prevention / Lung cancer / Smoking / Survival / CytochromeP450 1A1 / 遺伝子多型
Research Abstract

[Introduction] Cytochrome P450 (CYP) 2A6 and CYP2E1 are well-known enzymes that activate procarcinogens, such as the nitrosamines (solvents in tobacco smoke) and that form of DNA adduct. CYP2A6 and CYP2E1 mRNA are also observed in bronchial epithelial cells (BEG). In this study, CYP2A6 and CYP2E1 immunoreactivities in BEC and p53 immunoreactivities in tumor were examined in 78 non-small cell lung cancer (NSCLC) patients. We investigated the relationships between CYP2A6 and CYP2E1 expression in BEC and lifetime cigarette consumption (LTCC). We also revealed the relationships between positive numbers of CYP2A6 and CYP2E1 expression in BEC and LTCC.
[Materials and Methods] We examined CYP2A6 CYP2E1, p53 immunoreactivities in 78 Japanese patients with NSCLC who underwent surgical resection at UOEH after obtaining appropriate informed consent. Histological typing of the tumors was performed according to the WHO classification (adenocarcinoma ; 48 cases, squamous cell carcinoma ; 30 cases). T … More he amount of LTCC classified by the Brinkman Index (BI ; cigarettes/day x years). Immunohistochemical staining (IHC) was performed by the LSAB (labelled streptavidine biotin) method. CYP2A6 and CYP2E1 expression was determined as positive when more than 10% of BEC were stained and p53 expression as positive when more than 10% of tumor cells were stained.
[Results and conclusions] The CYP2A6 and CYP2E1 positive rates in 78 NSCLC were 53% (41/78) and 22% (17/78), respectively. The p53 positive rate was 53% (41/78). The CYP2A6 positive rate (60%) in 30 squamous cell carcinoma seemed to be higher than that (48%) in 48 adenocarcinoma. The p53 positive rate (57%) in 30 squamous cell carcinoma also seemed to be higher than that (50%) in 48 adenocarcinoma. There was a significant relation between CYP2E1 expression and LTCC (p<0.05). There was also a significant relation between positive numbers of CYP expression and LTCC (p<0.05). The CYP2A6 and CYP2E1 positive cases seemed to have shortened survival compared with the CYP2A6 and CYP2E1 negative cases in NSCLC patients with stage I disease.
To study CYP2A6 and CYP2E1 expression in BEC, it may be useful not only accurately to predict the risk of cancer development, but also to formulate individually-tailored plans for cancer prevention. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Oyama T, et al.: "Evidence-based prevention (EBP) : Approach to long cancer prevention based on cytochrome 1A1 and cytochrome 2E1 polymorphism."Anticancer Res. 23. 1731-1738 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oyama T, et al.: "A case-case study comparing the usefulness of serum trace elements (Cu, Zn and Se) and tumor markers (CRA, SCC and SLX) in non-small cell lung cancer patients."Anticancer Res. 23. 605-612 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawamoto T, et al.: "Effects of genetic polymorphism of drug metabolizing enzymes on smoking and drinking."J UOEH. 25. 97-106 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sagio K, et al.: "Genetic alteration in carcinoid tumors of the lung."Ann Thorac Cardiovasc Surg. 9. 126-129 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim Y-D, et al.: "Oxidative stress, hoggl expression and NF-kB activity in cells exposed to low level chronium."J Occup Health. 45. 271-277 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小山倫浩: "エビデンスに基づく予防-チトクローム2E1遺伝子多型に基づいた肺癌予防への取り組み-"産業医科大学雑誌. 24. 413-421 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto A, et al.: "Bisphenol A levels in human urine."Environ Health Persp. 111. 101-104 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oyama T, et al.: "Lung cancer and CYP1A1 or GSTM1 polymorphism."Environ Health Prev Med. 7. 230-234 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Osaki T, et al.: "Prognostic impact of micrometastatic tumor cells in the lymph nodes and bone marrow of patients with completely resected stage I non-small-cell lung cancer."J Clin Oncol. 20. 2930-2936 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Isse T, et al.: "Diminished alcohol preference in transgenic mice tacking aldehyde dehydrogenase activity."Pharmacogenetics. 12. 621-626 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Imahayashi S, et al.: "Establishment of an immortalized T-cell line from lung cancer tissne : phenotypic and functional analyses."Int J Clin Oucol. 7. 38-44 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Gabrilovich DI, et al.: "H1(0) histone and differentiation of dendritic cells. A molecular target for tumor-derived factors."J Leukocyte Biol. 72. 285-296 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oyama T, et al.: "Evidence-based prevention (EBP) : Approach to lung cancer prevention based on cytochrome 1A1 and cytochrome 2E1 polymorphism."Anticancer Res. 23. 1731-1738 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oyama T, et al.: "A case-case study comparing the usefulness of serum trace elements (Cu, Zn and Se) and tumor markers (CFA, SCC and SLX) in non-small cell lung cancer patients."Anticancer Res. 23. 605-612 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawamoto T, et al.: "Effects of genetic polymorphism of drug metabolizing enzymes on smoking and drinking."J UOEH. 25. 97-106 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sugio K, et al.: "Genetic alteration in carcinoid tumors of the lung."Ann Thorac Cardiovasc Surg. 9. 126-129 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim Y-D, et al.: "Oxidative stress, hogg1 expression and NF-κB activity in cells exposed to low level chronium."J Occup Health. 45. 271-277 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oyama T, et al.: "Evidence based prevention (EBP) : Evidence-based approach to prevention of lung cancer by application of Cytochrome 2E1 polymorphism."J UOEH. 24. 413-421 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto A, et al.: "Bisphenol A levels in human urine."Environ Health Persp. 111. 101-104 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oyama T, et al.: "Lung cancer and CYP1A1 or GSTM1 polymorphism."Environ Health Prev Med. 7. 230-234 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Osaki T, et al.: "Prognostic impact of micrometastatic tumor cells in the lymph nodes and bone marrow of patients with completely resected stage I non-small-cell lung cancer."J Clin Oncol. 20. 2930-2936 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Isse T, et al.: "Diminished alcohol preference in transgenic mice lacking aldehyde dehydrogenase activity."Pharmacogenetics. 12. 621-626 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Imahayashi S, et al.: "Establishment of an immortalized T-cell line from lung cancer tissue : phenotypic and functional analyses."Int J Clin Oncol. 7. 38-44 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Gabrilovich DI, et al.: "H1(0) histone and differentiation of dendritic cells. A molecular target for tumor-derived factors."J Leukocyte Biol. 72. 285-296 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oyama T, et al.: "Evidence-based prevention (EBP) ; Approach to lung cancer prevention based on cytochrome 1A1 and cytochrome 2E1 polymorphism."Anticancer Res. 23. 1731-1738 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Oyama T, et al.: "A case-case study comparing the usefulness of serum trace elements (Cu, Zn and Se) and tumor markers (CEA, SCC and SLX) in non-small cell lung cancer patients."Anticancer Res. 23. 605-612 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawamoto T, et al.: "Effects of genetic polymorphism of drug metabolizing enzymes on smoking and drinking."J UOEH. 25. 97-106 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sugio K, et al.: "Genetic alteration in carcinoid tumors of the lung."Ann Thorac Cardiovasc Surg. 9. 126-129 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kim Y-D, et al.: "Oxidative stress, hogg1 expression and NF-kB activity in cells exposed to low level chronium."J Occup Health. 45. 271-277 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小山倫浩ら: "エビデンスに基づく予防-チトクローム2E1遺伝子多型に基づいた肺癌予防への取り組み-"産業医科大学雑誌. 24. 413-421 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsumoto A, et al.: "Bisphenol A levels in human urine"Environ Health Persp. 111. 101-104 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Oyama T, et al.: "Lung cancer and CYP1A1 or GSTM1 polymorphism"Environ Health Prev Med. 7. 230-234 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Osaki T, et al.: "Prognostic impact of micrometastatic tumor cells in the lymph nodes and bone marrow of patients with completely resected stage I non-small-cell lung cancer"J Clin Oncol. 20. 2930-2936 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Isse T, et al.: "Diminished alcohol preference in transgenic mice lacking aldehyde dehydrogenase activity"Pharmacogenetics. 12. 621-626 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Imahayashi S, et al.: "Establishment of an immortalized T-cell line from lung cancer tissue : phenotypic and functional analyses"Int J Clin Oncol. 7. 38-44 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Gabrilovich DI, et al.: "H1(0) histone and differentiation of dendritic cells. A molecular target for tumor-derived factors"J Leukocyte Biol. 72. 285-296 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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