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Role of intracellular redox in crosstalk between TH1/TH2 balance and nature immunity

Research Project

Project/Area Number 14570403
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionGunma University

Principal Investigator

DOBASHI Kunio  Gunma University, School of Medicine, Assistant Professor, 医学部, 講師 (00241894)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsTH1 / TH2 balance / glutathione / IL-12 / oxidative stress / Toll-like receptor / GSH / GSSG / MAP kinase / レドックス / Th1・Th2バランス / MAPキナーゼ
Research Abstract

We recently demonstrated that balance of reduced and oxidized glutathione level (GSH/GSSG) in macrophage (MΦ) play a central role in determining which of the TH1 and TH2 cytokine responses predominate during immune states through IL-12 production. In this study, we investigated whether changes in intra-cellular GSH/GSSG balance regulate LPS-induced IL-12 production and defined the molecular mechanism that underlies glutathione redox regulation.
Glutathione redox regulates LPS-induced IL-12 production through p38 MAP kinase activation. (Ref 5) On the other hand, c-jun N-terminal kinase negatively regulates LPS-induced IL-12 production from MΦ, and glutathione redox regulates LPS-induced IL-12 production through the opposite control of JNK and p38 MAP kinase activation. (Ref 1)
And, we revealed that TH1 and TH2 cytokines up and down-regulated the expression of Toll-like receptor 4 respectively, and that the crosstalk between TH1・TH2 balance and innate immunity. (Ref 3)
Furthermore, TGF-β 1-induced CTGF mRNA expression in human lung fibroblasts is mediated through the JNK-dependent pathway. (Ref 2)

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Utsugi M: "c-Jun N-terminal kinase negatively regulates lipopolysaccharide-induced IL-12 production in human macrophages :"J Immunol.. 171. 628-635 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Utsugi M: "C-Jun-NH2-terminal kinase mediates expression of connective tissue growth factor induced by transforming growth factor-beta1 in human lung fibroblasts."Am J Respir Cell Mol Biol.. 28. 754-761 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mita Y: "Toll-like receptor 4 surface expression on human monocytes and B cells is modulated by IL-2 and IL-4"Immunol Lett.. 81. 71-75 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishizuka T: "Interleukin-5 messenger RNA expression in peripheral blood mononuclear cells from patients with bronchial asthma and eosinophilia."Allergy Asthma Proc.. 23. 175-179 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Utsugi M: "Glutathione redox regulates lipopolysaccharide-induced IL-12 production through p38 mitogen-activated protein kinase activation in human monocytes :"J Leukoc Biol.. 71. 339-347 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Utsugi M: "Ambroxol inhibits platelet-derived growth factor production in human monocytic cells."Eur J Pharmacol. 436. 47-51 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Utsugi M, Dobashi K, Ishizuka T, Endou K, Hamuro J, Murata Y, Nakazawa T, Mori M.: "c-Jun N-terminal kinase negatively regulates lipopolysaccharide-induced IL-12 production in human macrophages : role of mitogen-activated protein kinase in glutathione redox regulation of IL-12 production."J Immunol.. 171(2). 628-635 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Utsugi M, Dobashi K, Ishizuka T, Masubuchi K, Shimizu Y, Nakazawa T, Mori M: "C-Jun-NH2-terminal kinase mediates expression of connective tissue growth factor induced by transforming growth factor-beta1 in human lung fibroblasts."Am J Respir Cell Mol Biol.. 28(6). 754-761 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mita Y, Dobashi K, Endou K, Kawata T, Shimizu Y, Nakazawa T, Mori M.: "Toll-like receptor 4 surface expression on human monocytes and B cells is modulated by IL-2 and IL-4."Immunol Lett.. 81(1). 71-75 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishizuka T, Ishizuka T, Iwamae S, Hisada T, Okayama Y, Tsukagoshi H, Dobashi K, Mori M.: "Interleukin-5 messenger RNA expression in peripheral blood mononuclear cells from patients with bronchial asthma and eosinophilia."Allergy Asthma Proc.. 23(3). 175-179 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Utsugi M, Dobashi K, Koga Y, Shimizu Y, Ishizuka T, Iizuka K, Hamuro J, Nakazawa T, Mori M.: "Glutathione redox regulates lipopolysaccharide-induced IL-12 production through p38 protein kinase activation in human monocytes : role of glutathione redox in IFN-gamma priming of IL-12 production."J Leukoc Biol.. 71(2). 339-347 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Utsugi M, Dobashi K, Koga Y, Masubuchi K, Shimizu Y, Endou K, Nakazawa T, Mori M.: "Ambroxol inhibits platelet-derived growth factor production in human monocytic cells."Eur J Pharmacol.. 436(1-2). 47-51 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Utsugi M: "c-Jun N-terminal kinase negatively regulates lipopolysaccharide-induced IL-12 production in human macrophages:"J Immunol.. 171. 628-635 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Utsugi M: "C-Jun-NH2-terminal kinase mediates expression of connective tissue growth factor induced by transforming growth factor-betal in human lung fibroblasts."Am J Respir Cell Mol Biol.. 28. 754-761 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mita Y: "Toll-like receptor 4 surface expression on human monocytes and B cells is modulated by IL-2 and IL-4"Immunol Lett.;. 81. 71-75 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ishizuka T: "Interleukin-5 messenger RNA expression in peripheral blood mononuclear cells from patients with bronchial asthma and eosinophilia."Allergy Asthma Proc.. 23. 175-179 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Utsugi M: "Glutathione redox regulates lipopolysaccharide-induced IL-12 production through p38 mitogen-activated protein kinase activation in human monocytes:"J Leukoc Biol.. 71. 339-347 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Utsugi M: "Ambroxol inhibits platelet-derivd growth factor productin in human monocytic cells."Eur J Pharmacol. 436. 47-51 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Utsugi M., Dobashi, K.et al.: "Glutathione redox regulates lipoplysaccharide-induced IL-12 production through p38 mitogen-activated protein kinaseactivation in human monocytes : role of glutathione redox in IFN-g priming of IL-12 production"J. Leuko. Biol. 71. 339-347 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mita, Y., Dobashi K.et al.: "Toll-like receptor 4 surface expression on human monocytes and B cells is modulated by IL-2 and IL-4"Immunology Letters. 81. 71-75 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Utsugi M, Dobashi K, et al.: "Ambroxol inhibits platelet-derived growth factor production in human monocytic cells"Eur J Pharmacol. 436. 47-51 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mita, Y., Dobashi K.et al.: "Surface expression of toll-like receptor 4 on THP-1 cells is modulated by Bu-Zhong-Yi-Qi-Tang and Shi-Quan-Da-Bu-Tang"Methods Find Exp Clin Pharmacol.. 24. 67-70 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Utsugi M., Dobashi, K.et al.: "C-Jun-NH2-terminal kinases mediates expression of connective tissue growth factor induced by transforming growth factor-betal in human lung fibroblasts"Am. J. Respir. Cell Mol. Biol.. (In press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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