• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the components of lipid rafts and the possible alteration their pattern in SLE

Research Project

Project/Area Number 14570408
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionThe University of Tokyo

Principal Investigator

HONDA Zem-ichiro  The University of Tokyo, FAOM, Professor, 医学部附属病院, 講師 (70238814)

Co-Investigator(Kenkyū-buntansha) OKADA Masato  Osaka University, Research Inst. For Microbial, Dis. Professor, 微生物研究所, 教授 (10177058)
TSUCHIYA Noyuki  The University of Tokyo, Gr.Stud.. for Med. and FAOM, Associate Professor, 大学院・医学系研究科, 助教授 (60231437)
SUZUKI Takeshi  The University of Tokyo, FAOM, Assistant Professor, 医学部附属病院, 助手 (50272555)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordslipid rafts / FcγRIIB / systemic lupus erythematosus / Src family kinas / 脂質ラフト / 微小管 / Src family kinase / Fe受容体 / srcフォミリーキナーゼ / SH3ドメイン / FeγRIIb
Research Abstract

FcγRIIB, a tow-affinity lgG receptor, possesses inhibitory potential on B cell antigen receptor (BCR) signaling and contributes to balance immune responses in vivo. We have recently described a novel polymorphism, FcγRIIB lle232Thr, and its association with susceptibility to systemic lupus erythematosus (SLE) in Asians. Here we show that lle232Thr substitution impairs the ability of FcγRIIB to inhibit 8CR signaling related to phosphatidylinostol-(3 14,5)-trisphosphate synthesis. Intriguingly, Fcγ RIIB-232Thr showed reduced association with specialized membrane microdomains, referred to as lipid rafts, thus providing mechanistic basis for the reduced inhibitory potential of the allele. This is the first demonstration of disease-associated receptor polymorphism resulting in altered membrane compartmentalization, and may provide novel mechanisms that potentially contribute to aberrant B cell regulation in SLE patients possessing FCGR2B-232Thr/Thr genotype.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Kono H, et al., Honda Z: "Spatial raft coalescence represents an initial step in Fc gamma R signaling."The Journal of Immunology. 169. 193-203 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda S, Honda Z, et al.: "Critical roles of c-Kit tyrosine residues 567 and 719 in stem cell factor-induced chemotaxis : contribution of src family kinase and PI3-kinase on calcium mobilization and cell migration."Blood. 99. 3342-3349 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Honda Z, Ishii S, Shimizu T: "Platelet-activating factor receptor."Journal of Biochemistry. 131. 773-779 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kono H, Suzuki T, Yaniamoto K, Okada M, Yamamoto T, Honda Z.: "Spatial raft coalescence represents an initial step in Fc gamma R signaling."Journal of Immunology.. 169(1). 193-203 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ueda S, Mizuki M, ikeda H, Tsujimura T, Matsumura I, Nakano K, Daino H, Honda Z, Sonoyama J, Shibayama H, others.: "Critical roles of c-Kit tyrosine residues 567 and 719 in stem cell factor-induced chemotaxis: contribution of src family kinase and P13-kinase on calcium mobilization and cell migration."Blood. 99(9). 3342-3349 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Honda Z, Ishii S, Shimizu T.: "Platelet-activating factor receptor."Journal of Biochemistry. 131(6). 773-779 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kono H., Suzuki T., et al.: "Spatial Raftcoalescence represents an initial step in FeγR signaling"The Journal of Immunology. 169. 193-203 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Honda Z,. et al.: "platelet-activating faetor"J. Biochemistry. 131. 773-779 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ueda S., Honda Z., et al.: "Crotied voles of c-kittyrosineresidure"Blood. 99. 3342-3349 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi