| Project/Area Number |
14570409
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Tokyo medical and dental university, Medical Research Institute |
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Principal Investigator |
YAMAGUCHI Tokio Tokyo Medical & Dental University, Medical Research Institute, Associate Prof., 難治疾患研究所, 助教授 (30134745)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAOKA Masayuki Member of the rederation of Public Service & Affiliated Personal Aid Associations, Kudanzaka Hospital, Director os Psychosomatic Internal Medicine, 心療内科部長
SUEMATSU Makoto Keio University, School of Medicine, Professor, 医学部, 教授 (00206385)
SUGIMOTO Akiko Tokyo Medical & Dental University, Inst.Biomater. & Engineer., Associate Prof., 生体材料工学研究所, 助教授 (60143608)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | oxidative stress / biopyrrin / antioxidant / bilirubin / oxidative stress-marker / ビリルビン酸化生成物 / ストレス・マーカー / ヘム代謝 / 酸化ストレス / Bilirubin / Biliverdin / Bilirubin oxidative metabolites / Biopyrrin / Antioxidant / Oxidative stress / Psychological stress / Home oxygenase |
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| Research Abstract |
Some authors have suggested that psychological stress induces the production of reactive oxygen species(ROS). Some studies have supported that bilirubin exerts anti-oxidative effects in vivo. However, it is not known whether ROS induced by psychological stress provoke bilirubin oxidation in vivo. We investigated if the concentration of biopyrrin, a bilirubin oxidative metabolite, increased in urine from subjects exposed to psychological stress. Sixty healthy male volunteers working in a pharmaceutical company were divided into a Group I which did not attend a conference, a Group II which attended a conference but did not deliver a speech, and a Group III which attended a conference and delivered speeches in the presence of the company executives. Subjective stress was scored (self-rating score) after subjects in Group III delivered their speeches at the conference. Urine was collected on the next day. The biopyrrin concentrations, as measured by enzyme-linked immunosorbent assay, were normalized to the urinary concentration of creatinine. The concentration of biopyrrin in Group III was significantly higher compared to that in Groups I and II (p<0.01 and p<0.05, respectively). Furthermore, in Group III, the concentration of biopyrrin correlated with the self-rating stress score (r=0.53, p<0.01). These findings suggest that emotional stimuli are associated with an increase in the oxidative metabolites of bilirubin in human urine, and that biopyrrins could be useful markers of psychological stress. Based on these reports on the possible relationship among psychological stress, oxidative stress and bilirubin as the antioxidant, we examined the possibility that psychological stress contributed to the oxidative conditions, and the subsequent increase of the urinary concentration of biopyrrins produced by the reaction of bilirubin with ROS.
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