Inhibition of pulmonary eosinophilia by Mucosal plasmid DNA immunization
| Project/Area Number |
14570423
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Dept. of Internal Medicine II, Fukushima Medical University School of Medicine |
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Principal Investigator |
SATO Yukio Fukushima Medical University School of Medicine, Associate Professor of Medicine, 医学部, 教授 (20254013)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Hiroko Fukushima Medical University School of Medicine, Associate Professor of Medicine, 医学部, 助手 (00336463)
OHIRA Hiromasa Fukushima Medical University School of Medicine, Associate Professor of Medicine, 医学部, 講師 (90274951)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | DNA VACCINE / ALLERGY / EOSINOPHILIA / MUCOSAL IMMUNITY / 粘膜 / アレルギー / 粘膜免疫 / 好酸球 |
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| Research Abstract |
Immunization with plasmid DNA through mucosal routes, but not systemic routes, has been shown to generate T-helper-1 (Th1)-biased systemic as well as mucosal immunity at local and distant mucosal sites. Immunization with plasmid DNA encoding an allergen via the systemic routes has been shown to prevent activation of allergen-specific Th2 cells following injection of allergen protein in aluminum hydroxide, and to inhibit specific lgE synthesis. We attempted to determine whether Th1-biased mucosal immunity induced by mucosal plasmid DNA immunization inhibits pulmonary eosinophilia in ovalbumin (OVA)-sensitized mice after OVA inhalation. The results showed that immunization of Balb/c mice with plasmid DNA encoding OVA, pCMV-OVA, via mucosal route, but not via an intradermal (i. d.) route, enhanced interferon-γ (IFN-γ) levels in bronchoalveolar (BAL) fluid after OVA inhalation. Immunization with pCMV-OVA via mucosal routes prior to OVA sensitization inhibited serum lgE responses and pulmonary eosinophilia These results suggested that i.g. plasmid DNA immunization is capable of inducing Th1-biased mucosal immunity in bronchial mucosa and of inhibiting airway eosinophilia even in the presence of an ongoing Th2 response. Therefore, plasmid DNA immnunization via mucosal routes may be more effective than that via systemic routes in inhibiting pulmonary allergic responses.
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Report
(3 results)
Research Products
(14 results)
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[Publications] Nomaguchi H, Mukai T, Takeshita F, Matsuoka M, Maeda Y, Aye T-M, Jahan N, Yogi Y, Endo M, Sato Y, Makino M.: "Effect of hsp65 DNA vaccination carrying immunostimulatory DNA sequences (CpG motifs) against mycobacterium leprae multiplication in mice."Int J Leprosy. 70. 182-190 (2002)
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