• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Induction of cellular immune responses against hepatitis C Virus (HCV) and development of HCV vaccines.

Research Project

Project/Area Number 14570461
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionMie University

Principal Investigator

TAMAKI Shigenori  Mie University, Hospitals, Assistant, 医学部附属病院, 助手 (80260602)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsHCV / CTL / vaccine / alpha antigen / BCG / 肝炎
Research Abstract

Hepatitis C virus (HCV)-specific cytotoxic T lymphocytes (CTL) play important roles in the prevention of viral spread and viral clearance during HCV infection. The results of our study contributed to gain more insights into the pathogenesis of HCV infection and allowed the generation of various vaccines against HCV.
1. Establishment of novel animal model of HCV infection
Mice were intravenously inoculated with recombinant adenovirus encoding HCV structural proteins. Expression of HCV structural proteins in hepatocytes were detected immunohistochemically. These mice developed CD8(+) MHC class I-restricted CTLs against all three HCV structural proteins.
2. Recombinant BCG vaccine against HCV
We assessed the capacity of a novel recombinant vaccine using an attenuated tuberculosis bacillus. Calmette-Guerin bacillus (BCG) as a vaccine vehicle to elicit HCV-specific CTLs. BCG was engineered to express the CTL epitope of HCV-NS5a as a chimeric protein with alpha antigen of mycobacteria. Immunization with this recombinant BCG elicited MHC class I-resricted CD8(+) HCV-NS5a-specific CTLs in mice. Immunized mice showed a substantial reduction in the vaccinia virus titer compared with control mice when the immunized mice were challenged with a recombinant vaccinia virus expressing HCV-NS5a genes.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Nishikubo K: "A single administration of interleukin-4 antagonistic mutant DNA inhibits allergic airway inflammation in a mouse model of asthma."Gene Ther. 10. 2119-2125 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yuda H: "Activated Protein C inhibits bronchial hyperresponsiveness and Th2 cytokine expression in mice."Blood. 203. 2196-2204 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakashima S: "Vacuolar H+-ATPase inhibitor induces apoptosis via lysosomal dysfunction in the human gastric cancer cell line MKN-1."J Biochem (Tokyo). 134. 359-364 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uno-Furuta S: "Immunization with recombinant Calmette-Guerin bacillus (BCG)-hepatitis C virus (HCV) elicits HCV-specific cytotoxic T lymphocytes in mice."Vaccine. 21. 3149-3156 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamisako T: "Serum platelet-activating factor acetylhydrolase (PAF-AH) activity in patients with hyperbilirubinemic hepatobiliary disease."Hepatol Res. 26. 23-27 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishikubo K: "A single administration of interleukin-4 antagonistic mutant DNA inhibits allergic airway inflammation in a mouse model of asthma."Gene Ther. 10. 2119-2125 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yuda H: "Activated protein C inhibits bronchial hyperresponsiveness and Th2 cytokine expression in mice."Blood. 203. 2196-2204 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakashima S: "Vacuolar H+-ATPase inhibitor induces apoptosis via lysosomal dysfunction in the human gastric cancer cell line MKN-l."J Biochem (Tokyo). 134. 359-364 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uno-Furuta S: "Immunization with recombinant Calmette-Guerin bacillus (BCG)-hepatitis C virus (HCV) elicits HCV-specific cytotoxic T lymphocytes in mice."Vaccine. 21. 3149-3156 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kamisako T: "Serum platelet-activating factor acetylhydrolase (PAF-AH) activity in patients with hyperbilirubinemic hepatobiliary disease."Hepatol Res. 26. 23-27 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishikubo K: "A single administration of interleukin-4 antagonistic mutant DNA inhibits allergic airway inflammation in a mouse model of asthma"Gene Ther.. 10・26. 2119-2125 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yuda H: "Activated protein C bronchial hyperresponsiveness and Th2 cytokine expression in mice"Blood. 103・6. 2196-2204 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakashima S: "Vacuolar H+-ATPase inhibitor apoptosis via lysosomal dysfunction in the human gastric cancer cell line MKN-1"J Biochem (Tokyo). 134・3. 359-364 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Uno-Fruta S: "Immunization with recombinant Calamette-Guerin bacillus (BCG)-hepatitis C virus (HCV) elicits HCV-specific cytotoxic T lymphocytes in mice"Vaccine. 21・23. 3149-3156 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kamisako T: "Serum platelet-activating factor acetyhydrolase (PAF-AH) activity in patients with hyperbili rubinemic hepatobiliary disease"Hepatol Res.. 26・1. 23-27 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ikoma J: "Early diagonosis of hepatocellular carcinoma using a sensitive assya for serum des-gamma-carboxy prothrombin : a prospective study"Hepatogastroenterlogy. 39・43. 235-238 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Satori Uno-Furuta: "Immunization with recombinant Calmett-Guerin bacillus(BCG)-hepatitis C virus(HCV)elicits HCV-specific cytotoxic T lymphocytes in mice"Vaccine. (in press).

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi