| Project/Area Number |
14570467
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | OSAKA UNIVERSITY |
|---|
Principal Investigator |
KISO Shinichi Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (40335352)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUKUI Kouji Osaka University Graduate School of Medicine, Medical Staff, 医学部附属病院, 医員(臨床研究)
IGURA Takumi Osaka University Graduate School of Medicine, Medical Staff, 医学部附属病院, 医員(臨床研究)
TAMURA Shinji Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (30243223)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
|
|---|
| Keywords | Bone marrow cells / hepatocytes / trans-differentiation / bFGF / HGF / 再生医療 / 肝幹細胞 / 増殖因子 / 肝前駆細胞 / 分化誘導 |
|---|
| Research Abstract |
Evidence that bone marrow cells have trans-differencing potential to hepatocytes have been described in recent reports. However, the molecular mechanism underlying this phenomenon is unclear. To address this issue, we investigated the parameters involved in the trans-differentiation ob bone marrow cells into hepatic lineage. (Methods) Mouse BM cells were cultured in a collagen gel without or with growth factors, including basic fibroblast growth factor. The expression of hepatocyte-specific markers, cholangiocyte-specific marker and liver-enriched transcription factors was identified by RT-PCR and immunohistochemistry. (Results) Basic fibroblast growth factor was found to be the most effective for inducing albumin in cultured BM cells. Furthermore, on stimulation of basic fibroblast growth factor, BM cells were found to express other hepatocyte-specific markers and a cholangiocyte-specific marker. This conversion was found to be associated with the induction of transcription factors including hepatocyte nuclear factors and GATA family proteins. (Conclusion) We established an in vitro culture system in which mouse bone marrow cells could trans-differentiate to hepatic lineage cells in response to growth factors. In particular, basic fibroblast growth factor has the ability to induce the trans-differentiation into hepatic lineage cells from BM cells.
|
