| Project/Area Number |
14570504
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Saitama Medical School |
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Principal Investigator |
MATSUI Atsushi Saitama Medical School, Faculty of Medicine, Assistant, 医学部, 講師 (40260484)
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| Co-Investigator(Kenkyū-buntansha) |
INAO Mie Saitama Medical School, Faculty of Medicine, Assistant, 医学部, 助手 (70286037)
MOCHIDA Satoshi Saitama Medical School, Faculty of Medicine, Professor, 医学部, 教授 (20219968)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Angiopoietin / Tie-2 / Sinusoidal endothelial cells / Stellate cells / Kupffer cells / Sinusoids / Hepatectomized rats / Immunohistochemistry / 障害肝 / 類洞 / KUPFFER細胞 |
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| Research Abstract |
Sinusoidal endothelial cells and hepatocytes increase in number after partial resection and necrosis of the liver, and contribute to both reconstruction and capillarization of the sinusoids through interaction with stellate cells. The mechanism underling such interaction was evaluated regarding angiopoietins and Tie receptors essential for blood vessel formation. Hepatic mRNA expressions of angiopoietin-1, angiopoietin-2 and Tie-2 were increased at 168 hours after 70% liver resection with sinusoidal reconstruction in rats, and also at 48 hours with sinusoidal capillarization in the liver of rats given carbon tetrachloride(CC14). Tie-2 mRNA expression was detected in sinusoidal endothelial cells and stellate cells isolated from normal rats, and in activated stellate cells from CC14-intoxicated rats. The mRNA expressions of angiopoietin-1 and angiopoietin-2 were detectable in Kupffer cells, sinusoidal endothelial cells and stellate cells in normal rats, but increased in activated stellate cells and macrophages after CC14-intoxication. Both angiopoietins and Tie-2 were immunohistochemically stained along the sinusoids of 70% hepatectomized rats and in necrotic areas of CC14-intoxicated rats. Angiopoietin-1 and angiopoietin-2 may be involved in both reconstruction and capillarization of the sinusoids in rat liver after partial resection and necrosis through interaction between stellate cells and sinusoidal and vascular endothelial cells via Tie-2 receptor.
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