Distribution and characterization of IgG Fc binding protein in various mucin producing cells

• Project/Area Number: 14570506
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: KEIO UNIVERSITY
• Principal Investigator: OGATA Haruhiko Keio University, School of Medicine, Assistant professor, 医学部, 助手 (30177117)
• Co-Investigator(Kenkyū-buntansha): HIBI Toshifumi Keio University, School of Medicine, Professor, 医学部, 教授 (50129623)
• Project Period (FY): 2002 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000); Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: mucosal defense / mucin / IgG Fc binding protein / intestinal mucosal immunology

Research Abstract

Immunohistochemical localisation of Fc binding protein (FcBP), using monoclonal antibodies against FcBP was conducted in various mucin-producing tissues. The binding activity of FcBP in mucus extracted from mucin-producing tissues was also examined by immunodotblot and immunoprecipitation using these monoclonal antibodies. Inhibition of complement mediated haemolysis by FcBP was investigated using sheep red blood cells (SRBC) and anti-SRBC IgG. The immunohistochemical study revealed that mucin-secreting cells in the colon, small intestine, gall bladder, cystic duct, choledochus, bronchus, submandibular gland, and cervix uteri contained FcBP, and immunodotblot and immunoprecipitation analysis using IgG and monoclonal antibodies demonstrated that the fluids secreted by these cells were capable of binding IgG. Mucin producing cells of the conjunctiva did not express FcBP molecules or bind to IgG. The surface mucus cells in the stomach were variably positive for FcBP. Perhaps because of proteolytic degradation, FcBP in gut lavage fluid did not have IgG binding activity, although this activity was present in the mucus covering the colon. FcBP suppressed complement mediated haemolysis of SRBC. Thus FcBP is widely expressed on mucosal surfaces and in external secretions. It is functionally intact in several fluids. These findings lend support to the concept that FcBP is an important component of mucosal immunological defenses.

Research Products

• [Publications] Takagi H, et al.: "Contrasting action of IL-12 and IL-18 in the development of dextran sodium sulphate colitis in mice."Scand J Gastroenterol. 8. 837-844 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ezaki T, et al.: "A specific genetic alteration on chromosome 6 in ulcerative colitis-associated colorectal cancers."Cancer Research. 63. 3747-3749 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Naganuma et al.: "Characterization of structures with T-lymphocyte aggregates in ileal villi of Crohn's disease."Am J Gastroenterol. 97(7). 1741-1747 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kobayashi K, et al.: "Distribution and partial characterization of IgG Fc binding protein in various mucin producing cells and body fluids"Gut. 51(2). 169-176 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takagi H, Kanai T, Okazawa A, Kishi Y, Takaishi H, Inoue N, Ogata H, Iwao Y, Hoshino K, Takeda K, Akira S, Watanabe M, Ishii H, Hibi T: "Contrasting action of IL-12 and IL-18 in the development of dextran sodium sulphate colitis in mice."Scand J Gastroenterol. 8. 837-844 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ezaki T, Watanabe M, Kanai T, Ogata H, Iwao Y, Ishii H, Hibi T: "A specific genetic alteration on chromosome 6 in ulcerative colitis-associated colorectal cancers."Cancer Research. 63. 3747-3749 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Naganuma M, Watanabe M, Kanai T, Ogata H, Iwao Y, Mukai M, Ishii H, Hibi T: "Characterization of structures with T lymphocyte aggregates in ileal villi of Crohn's disease."Am J Gastroenterol. 97. 1741-1747 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kobayashi K, Ogata H, Iijima S, Morikawa M, Harada N, Yoshida T, Brown WR, Inoue N, Hamada Y, Ishii H, Watanabe M, Hibi T: "Distribution and partial characterization of IgG Fc binding protein in various mucin producing cells and body fluids."Gut. 51. 169-176 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Naganuma M, et al.: "Granulocytapheresis is useful as an alterative therapy in patients with steroid-refractory or dependent ulcerative colitis."Inflamm Bowel Dis. in press. (2004)
• [Publications] Takagi H, et al.: "Contrasting action of IL-12 and IL-18 in the development, of dextran sodium sulphate colitis in mice."Scand J Gastroenterol. 8. 837-844 (2003)
• [Publications] Ezaki T, et al.: "A specific genetic alteration on chromosome 6 in ulcerative colitis-associated colorectal cancers."Cancer Research. 63. 3747-3749 (2003)
• [Publications] Kobayashi K, et al.: "Distribution and partial characterization of IgG Fc binding protein in various mucin producing cells and body fluids"Gut. 51(2). 169-176 (2002)
• [Publications] Okamoto R, et al.: "Damaged epithelia regenerated by bone marrow-derived cells in the human gastrointestinal tract"Nature Med. 8(9). 1011-1017 (2002)
• [Publications] Naganuma et al.: "Characterization of structures with T-lymphocyte aggregates in ileal villi of Crohn's disease"Am J Gastroenterol. 97(7). 1741-1747 (2002)