STAT3-TARGETED THERA PEUTIC APPROACH IN INFLAMMATORY BOWEL DISEASE
| Project/Area Number |
14570527
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kurume University |
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Principal Investigator |
MITSUYAMA Keiichi Kurume University School of Medicine, lecturer, 医学部, 講師 (20200066)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Asuka Kurume University School of Medicine, 医学部, 助手 (30341324)
TOMIYASU Nobuo Kurume University School of Medicine, 医学部, 助手 (20289424)
NISHIYAMA Tsutomu Kurume University School of Medicine, 医学部, 助手 (70279157)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | inflammatory bowel disease / STAT3 / interleukin 6 / gp130 |
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| Research Abstract |
We have already reported that IL6/STAT3 signaling pathway plays a key role in the development of human inflammatory bowel disease as well as animal models of intestinal inflammation, and speculated that the regulation of STAT3 activity may be a promising method of therapeutic intervention. The aim of this study was to elucidate the expression of IL6/STAT3 signaling pathway and the effect of IL6/soluble IL6 receptor fusion protein (hyper-IL6) and soluble gp13O (sgp13O) in SAMP1/Yit mice with experimental enterocolitis. The intestinal expression of phospho-STAT3 was examined by Western-blotting and immunohistochemistry. The effect of intravenous administration of hyper-IL6 or sgp13O on the severity of intestinal inflammation was assessed clinically and histologically. The expression of phospho-STAT3 were pronounced in the inflamed mucosa. The presence of STAT3 was demonstrated in epithelial cells and in mononuclear cells. Injection of hyper-IL6 up-regulated phospho-STAT3 expression and exacerbated intestinal inflammation. On the contrary, administration of sgp13O down-regulated phospo-STAT3 expression and reduced the severity of intestinal inflammation. These results indicate that IL6/STAT3 signaling pathway plays a key role in the development of the intestinal inflammation and may be a promising target of therapeutic intervention.
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Report
(3 results)
Research Products
(27 results)
