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Increase in chemo-and radiotherapy and cancer gene therapy by means of suppression of DNA repair genes

Research Project

Project/Area Number 14570540
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionChiba University

Principal Investigator

TAKIGUCHI Yuichi  Chiba University, University Hospital, Lecturer, 医学部附属病院, 講師 (30272321)

Co-Investigator(Kenkyū-buntansha) KUROSU Katsushi  Chiba University, Graduate School of Medicine, Assistant (20291106)
TATSUMI Koichiro  Chiba University, Graduate School of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (10207061)
KURIYAMA Takayuki  Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (20009723)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsDNA repair gene / Ku70 / antisense oligonucleotide / radiosensitivity / chemosensitivity / gene therapy / lung cancer
Research Abstract

Ku70 protein, cooperating with Ku80 and DNA-dependent protein kinase (DNA-PK) catalytic subunits (DNA-PKcs), is involved in DNA double-strand break (DNA DSB) repair and V(D)J recombination. Recent studies have revealed increased ionizing radiosensitivity in Ku70-deficient cells. The presented study, using a human squamous cell lung carcinoma cell line, demonstrated that introduction of an antisense Ku70 nucleic acid made the cells more radio-and chemosensitive than the parental cells. Ku70 protein expression was suppressed in the cells with antisense Ku70 construct when compared to the wild type cells : A relatively small but statistically significant increase in radiosensitivity of the cells was achieved by the introduction of the antisense Ku70. The increased radiosensitivity in vitro was accompanied by an approximately two-fold increase in a and alb values in a linear-quadratic model. The antisense Ku70 increased the chemosensitivity of the cells to some DNA-damaging agents such as bleomycin and methyl methanesulfonate, but not to cisplatin, mitomycin C, and paclitaxel. This system provides us with partial suppression of Ku70, and will be a useful experimental model for investigating the physiological roles of the DNA DSB repair gene.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Omori S., Takiguchi Y.et al.: "Suppression of a DNA double-strand break repair gene, Ku70, increases radio-and chemosensitivity in a human lung carcinoma cell line"DNA Repair. 1. 299-310 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suda A., Takiguchi Y.et al.: "Gene delivery to the lung by means of syngeneic fibroblasts : an experimental model."Experimental Lung Research. 29・4. 227-241 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shingyoji M., Takiguchi Y.et al.: "Detection of tumor specific gene expression in bone marrow and peripheral blood from patients with small cell lung carcinoma."Cancer. 97・4. 1057-1062 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe R., Takiguchi Y.et al.: "Feasibility of combination chemotherapy with cisplatin and etoposide for haemodialysis patients with lung cancer."British Journal of Cancer. 88・1. 25-30 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tada Y., JO.Wang, Wada A., Takiguchi Y.et al.: "Fas ligand-expressing tumors induce tumor-specific protective immunity in the inoculated hosts but vaccination with the apoptotic tumors suppresses antitumor immunity."Cancer Gene Therapy. 10・2. 134-140 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takiguchi Y.: "Hiccups as an adverse reaction to cancer chemotherapy."Journal of the National Cancer Institute. 94・10. 772-772 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Omori S.Takiguchi Y.et al.: "Suppression of a DNA double-strand break repair gene, Ku70, increases radio-and chemosensitivity in a human lung carcinoma cell line"DNA Repair. 1. 299-310 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suda A., Takiguchi Y.et al.: "Gene delivery to the lung by means of syngeneic fibroblasts : an experiment model."Experimental Lung Research.. 29. 227-241 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shingyoji M., Takiguchi Y.et al.: "Detection of tumor specific gene expression in bone marrow and periphery blood from patients with small cell lung carcinoma."Cancer.. 97. 1057-1062 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe R., Takiguchi Y.et al.: "Feasibility of combination chemotherapy with cisplatin and etoposide for haemodialysis patients with lung cancer."British Journal of Cancer. 88. 25-30 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tada Y., J O.Wang, Wada A., Takiguchi Y.et al.: "Fas ligand-expressing tumors induce tumor-specific protective immunity in the inoculated hosts bit vaccination with the apoptotic tumors suppresses antitumor immunity."Cancer Gene Therapy.. 10. 134-140 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takiguchi Y. et al.: "Hiccups as an adverse reaction to cancer chemotherapy."Journal of the National Cancer Institute. 94. 772-772 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Omori S., Takiguchi Y.et al.: "Suppression of a DNA double-strand break repair gene, Ku70, increases radio-and chemosensitivity in a human lung carcinoma cell line"DNA Repair. 1. 299-310 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suda A., Takiguchi Y.et al.: "Gene delivery to the lung by means of syngeneic fibroblasts : an experimental model."Experimental Lung Research. 29・4. 227-241 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shingyoji M., Takiguchi Y.et al.: "Detection of tumor specific gene expression in bone marrow and peripheral blood from patients with small cell lung carcinoma."Cancer. 97・4. 1057-1062 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Watanabe R., Takiguchi Y.et al.: "Feasibility of combination chemotherapy with cisplatin and etoposide for haemodialysis patients with lung cancer."British Journal of Cancer. 88・1. 25-30 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tada Y., J O.Wang, Wada A., Takiguchi Y.et al.: "Fas ligand-expressing tumors induce tumor-specific protective immunity in the inoculated hosts but vaccination with the apoptotic tumors suppresses antitumor immunity."Cancer Gene Therapy. 10・2. 134-140 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takiguchi Y.: "Hiccups as an adverse reaction to cancer chemotherapy."Journal of the National Cancer Institute. 94・10. 772-772 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Omori S.: "Suppression of a DNA double-strand break repair gene, Ku70, increases radio-and chemosensitivity in a human lung carcinoma cell line"DNA Repair. 1. 299-310 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tada Y.: "Antitumor effects are produced by forced expression of membrane-bound but not soluble Fas ligand in murine lung carcinoma cells"Anticancer Research. 22・2A. 831-836 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tada Y.: "Expression of the TNF-alpha gene on mouse lung carcinoma cells suppresses spontaneous lung metastasis without affecting tumorigenicity"Oncology Reports. 9・3. 585-588 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kurasawa K: "Activation of pulmonary T cells in corticosteroid-resistant and -sensitive interstitial pneumonitis in dermatomyositis/polymyositis"Clinical & Experimental Immunology. 129・3. 541-548 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tada Y.: "Cutting edge : a novel role for Fas ligand in facilitating antigen acquisition by dendritic cells"Journal of Immunology. 169・5. 2241-2245 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takiguchi Y.: "Hiccups as an adverse reaction to cancer chemotherapy"Journal of the National Cancer Institute. 94・10. 772-772 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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