| Project/Area Number |
14570739
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Nagoya University |
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Principal Investigator |
KAMACHI Yoshiro Nagoya University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (20343204)
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Seiji Nagoya University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (20313992)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | DNA microarray / childhood / myelodysplastic syndrome / juvenile myelomonocytic leukemia / leukemoid reaction / CD34 positive cell / ウイルス感染 |
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| Research Abstract |
We have very difficult cases to distinguish juvenile myelomonocytic leukemia (JMML) of the infancy with leukemoid reaction by virus infection, because we have not yet established useful diagnostic method to differentiate between these two diseases. In this study, we analyzed the gene expression pattern of CD34^+ cells of the bone marrow of patients with JMML using DNA microarray method. We extracted RNA from CD34^+ cells of the bone marrow of JMML patients before and after treatment. We applied amplified cRNA labeled with fluorescent to DNA microarray slide. We found that the expression level of FRAP(FKBP-rapamycin associated protein) and Syk(spleen tyrosine kinase) increased significantly after treatment and that the expression level of c-jun, TGF-beta receptor III(transforming growth factor-beta receptor III) and IL-2(interleukin 2) decreased significantly after treatment. All of these five genes are involved with intracellular signal transduction, transformation, differentiation, and propagation. We conclude that DNA microarray method is useful for examination of a change of gene expression in JMML patients before and after treatment.
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