| Project/Area Number |
14570743
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
HARA Junichi Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助教授 (00238156)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Yoshiko Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (60343258)
FUJISAKI Hiroyuki Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 医員(臨床研究)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
|
|---|
| Keywords | Leukemic stem cell / hematopoietic stem cell / DNA miroarray / CD34+ CD38- / IL-8 / MCL-1 / MONO-7 / CD34+ CD38- / マイクロアレイ / ABCG2 / 白血病 / 幹細胞 |
|---|
| Research Abstract |
(Background)Recent studies reported that leukemic stem cells were identified and purified as CD34+ CD38- cells from various samples of patients with acute myelogenous leukemia and B-lineage acute lymphoblastic leukemia. The marker combination has been used isolate nearly pure hematopoietic stem cells. We investigated the biological characteristics of leukemic stem cells. (Methods)We examined a monosomy 7 leukemia cell line, designated MONO-7 which was established from the peripheral blood of a patient with monosomy 7 acute myelogenous leukemia. The CD34+ CD38- and CD34+ CD38+ subfraction of MONO-7 were enriched by magnetic activated cell sorting (MACS) system followed by purification through high-speed cell sorter (FACS Vantage). To compare the gene expression profile of these fractions, we used a DNA microarray technology, (Results)Interleukin-8 (IL-8), CCAAT/enhancer binding protein gamma (C/EBP γ) and induced myeloid leukemia cell differentiation protein (MCL-1) dominantly expressed in the CD34+ CD38- subfraction and CD34, leukocyte adhesion glycoprotein (LFA-1), CD 9 and eph-related receptor tyrosine kinase ligand 1 precursor (ephrin-A1) dominantly expressed in the CD34+ CD38+ subfraction.
|
