Detection and characterization of leukemic stem cells

• Project/Area Number: 14570743
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Osaka University
• Principal Investigator: HARA Junichi Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助教授 (00238156)
• Co-Investigator(Kenkyū-buntansha): MATSUDA Yoshiko Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (60343258) ; FUJISAKI Hiroyuki Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 医員(臨床研究)
• Project Period (FY): 2002 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥2,900,000 (Direct Cost: ¥2,900,000); Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: Leukemic stem cell / hematopoietic stem cell / DNA miroarray / CD34+ CD38- / IL-8 / MCL-1 / MONO-7 / CD34+ CD38- / マイクロアレイ / ABCG2 / 白血病 / 幹細胞

Research Abstract

(Background)Recent studies reported that leukemic stem cells were identified and purified as CD34+ CD38- cells from various samples of patients with acute myelogenous leukemia and B-lineage acute lymphoblastic leukemia. The marker combination has been used isolate nearly pure hematopoietic stem cells. We investigated the biological characteristics of leukemic stem cells. (Methods)We examined a monosomy 7 leukemia cell line, designated MONO-7 which was established from the peripheral blood of a patient with monosomy 7 acute myelogenous leukemia. The CD34+ CD38- and CD34+ CD38+ subfraction of MONO-7 were enriched by magnetic activated cell sorting (MACS) system followed by purification through high-speed cell sorter (FACS Vantage). To compare the gene expression profile of these fractions, we used a DNA microarray technology, (Results)Interleukin-8 (IL-8), CCAAT/enhancer binding protein gamma (C/EBP γ) and induced myeloid leukemia cell differentiation protein (MCL-1) dominantly expressed in the CD34+ CD38- subfraction and CD34, leukocyte adhesion glycoprotein (LFA-1), CD 9 and eph-related receptor tyrosine kinase ligand 1 precursor (ephrin-A1) dominantly expressed in the CD34+ CD38+ subfraction.

Research Products

• [Journal Article] Defective long-term repopulating ability in hematopoietic stem cells lacking the Polycomb-group gene rae28. (2004)
  • Author(s): Kim, J.Y., Sawada, A., Tokimasa, S., Endo, H., Ozono, K., Hara, J., Takihara, Y.
  • Journal Title: Eur J Haematol 73 Pages: 75-84
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Defective long-term repopulating ability in hematopoietic stem cells lacking the Polycomb-group gene rae28. (2004)
  • Author(s): Kim, J.Y.Sawada, A.Tokimasa, S.Endo, H.Ozono, K.Hara, J.Takihara, Y.
  • Journal Title: Eur J Haematol 73 Pages: 75-84
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Establishment of a monosomy 7 leukemia cell line, MONO-7, with a ras gene mutation. (2002)
  • Author(s): Fujisaki, H., Takai, K., Akihisa, S., Tokimasa, S., Matsuda, Y., Ohta, H., Osugi, Y., Kim, J.Y., Sako, M., Hara, J.
  • Journal Title: Int J Hematol 75 Pages: 72-77
  • NAID: 10007867337
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Polycomb group gene rae28 is required for sustaining activity of hematopoietic stem cells. (2002)
  • Author(s): Ohta, H., Sawada, A., Kim, J.Y., Tokimasa, S., Nishiguchi, S., Humphries, R.K., Hara, J., Takihara Y.
  • Journal Title: J Exp Med 195 Pages: 759-770
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Establishment of a monosomy 7 leukemia cell line, MONO-7, with a ras gene mutation. (2002)
  • Author(s): Fujisaki H, Takai, K.Akihisa, S.Tokimasa, S.Matsuda, Y.Ohta, H Osugi, Y.Kim, J.Sako M.Hara, J.
  • Journal Title: Int J Hematol 75 Pages: 72-77
  • NAID: 10007867337
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Polycomb group gene rae28 is required for sustaining activity of hematopoietic stem cells. (2002)
  • Author(s): Ohta, H.Sawada, A.Kim, J.Y.Tokimasa, S.Nishiguchi, S.Humphries, R.K.Hara, J.Takihara, Y.
  • Journal Title: J Exp Med 195 Pages: 759-770
  • Description: 「研究成果報告書概要(欧文)」より