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Cellular immunity of mother-to-infant infection and strain specificities of human cytomegalovirus strains obtained from

Research Project

Project/Area Number 14570756
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionSapporo Medical University

Principal Investigator

NUMAZAKI Kei  Sapporo Medical University, School of Medicine, Department of Pediatrics, Assistant Professor, 医学部, 講師 (00228272)

Co-Investigator(Kenkyū-buntansha) TSUTSUMI Hiroyuki  Sapporo Medical University, School of Medicine, Department of Pediatrics, Professor, 医学部, 教授 (80217348)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordscytomegalovirus(CMV) / intracellular cytokines / interferon-gamma / tumor necrosis factor-alpha / cellular immunity
Research Abstract

To determine the ability of immunological response to human cytomegalovirus(CMV), the flow cytometric assay was evaluated as a tool for real-time monitoring of specific cellular immunity in children with congenital GMV infection. Longitudinal cohort study of 2 children with asymptomatic and 2 with symptomatic congenital CMV infection evaluated at birth and followed up with serial age-appropriate neurodevelopmental testing. Frequencies of CMV-specific CD4(+) T cell in these children were detected by intracellular cytokines(ICC), interferon(IFN)-gamma and tumor necrosis factor(TNF)-alpha, staining. Findings detected by CT and MRI were the most sensitive predictor for neurodevelopmental prognosis. Frequencies of CMV-specific CD4(+) T cells detected by ICC, both IFN-gamma and TNF-alpha, were higher in 2 children with symptomatic congenital CMV infection than those in 2 children with asymptomatic congenital infection. Frequencies of CMV-specific CD4(+) T cells in 2 children with symptomatic congenital infection were significantly higher than those in 6 healthy children of 1 to 5-years of age with serum anti-CMV IgG antibody without serum anti-CMV IgM antibody and viral excretion into urine(p<0.01). The ICC assay reflects immunological activity against CMV infection in children with asymptomatic or symptomatic congenial infection. Categorizing findings obtained by the ICC assay may helps to determine the prognosis of children with congenital CMV infection.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Numazaki K, Fujikawa T.: "Prevalence of serum antibodies to cytomegalovirus in pregnant women in Sapporo Japan"Int J Infect Dis. 6. 147-148 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K, Fujikawa T, Asanuma H: "Immunological evaluation and clinical aspects of children with congenital cytomegalovirus infection."Congenital Anomalies. 42. 181-186 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujikawa T, Numazaki K, Asanuma H, Tsutsumi H: "Human cytomegalovirus infection during pregnancy and detection of specific T cells by intracellular cytokine staining"Int J Infect Dis. 7. 215-221 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujikawa T, Numazaki K, Asanuma H, Kudo R, Tsutsumi H: "The frequency of human cytomegalovirus-specific T cells during pregnancy determined by intracellular cytokine staining"J Med Virol. 71. 527-531 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K, Umetsu M, Adachi N: "Mycoplasma pneumoniae infection and its genotypical characterization in children of Hokkaido Japan"In Vivo. 17. 421-424 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K.: "Glycyrrhizin therapy of viral infections."Afr J Biotechnol. 2. 392-393 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 沼崎 啓: "今日の診断指針"東京, 医学書院. 2092 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K, Fujikawa T.: "Prevalence of serum antibodies to cytomegalovirus in pregnant women in Sapporo, Japan."Int J Infect Dis. 6. 147-148 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K, Fujikawa T, Asanuma H.: "Immunological evaluation and clinical aspects of children with congenital cytomegalovirus infection."Congenital Anomalies. 42. 181-186 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K, Umetsu M, Adachi N.: "Mycoplasma pneumoniae infection and its genotypical characterization in children of Hokkaido, Japan."In Vivo. 17. 421-424 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujikawa T, Numazaki K, Asanuma H, Tsutsumi H.: "Human cytomegalovirus infection during pregnancy and detection of specific T cells by intracellular cytokine staining."Int J Infect Dis. 7. 215-221 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K, Fujikawa T.: "Intracranial calcification with congenital rubella syndrome in a mother with serologic immunity."J Child Neurol. 18. 296-297 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujikawa T, Numazaki K, Asanuma H, Kudo R, Tsutsumi H.: "Frequency of human cytomegalovirus-specific T cells during pregnancy determined by intracellular cytokine staining."J Med Virol. 71. 527-531 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K, Asanuma H, Niida Y.: "Chlamydia trachomatis infection in early neonatal period."BMC Infectious Diseases. 3. 2(1-5) (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K.: "Prevalence of Bartonella infection among patients with fever."Afr.J.Biotechnol.. 2. 390-391 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K.: "Glycyrrhizin therapy for viral infections."Afr.J.Biotechnol.. 2. 392-393 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K.: "Retrospective diagnosis of congenital cytomegalovirus infection and cortical maldevelopment."Correspondence to Neurology. November 5. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K, Chiba S, Umetsu M, Tanaka T, Yoshimura H, Kuniya Y, Miura J, Adachi N, Ukae S, Mori T, Ueda D, Hotsubo T, Sato T.: "Etiological agents of lower respiratory tract infections in Japanese children."In Vivo. 18. 67-72 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K.: "Current problems of perinatal Chlamydia trachomatis infections."J Immune Based Ther Vaccines. 2. 4 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K, Asanuma H, Niida Y.: "Respiratory tract infections due to Chlamydia trachomatis in early neonatal period. The Third Symposium on Chlamydial Infections"Proceedings of the Third Joint Auspices of Japan Society for Chlamydia Research and Department of Pathobiology, University of Washington. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K.: "Immunological evaluation of children with congenital cytomegalovirus infection and infected women during pregnancy."Current Trends in Immunology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K.: "Liver dysfunction due to cytomegalovirus associated with Chlamydia trachomatis and respiratory syncytial virus infections."Clinical Microbiology Newsletter. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K, Kubo S.: "Infantile Chlamydia trachomatis pneumonia in siblings."Clinical Microbiology Newsletter. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K.: "Human cytomegalovirus infections in premature infants by breastfeeding."Newborn and Infant Nursing Reviews. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K.: "Bartonella henselae and Coxiella burnetii infection and Kawasaki disease."Journal of Applied Sciences and Environmental Management. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K.: "Adoptive immunotherapy for cytomegalovirus infection."Journal of Applied Sciences and Environmental Management. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K.: "Chlamydia trachomatis serovars of endemic trachoma had been predominantly existed in Japan?"Journal of Applied Sciences and Environmental Management. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Numazaki K, Fujikawa T.: "Prevalence of serum antibodies to cytomegalovirus in pregnant women in Sapporo Japan"Int J Infect Dis. 6. 147-148 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Numazaki K, Fujikawa T, Asanuma H: "Immunological evaluation and clinical aspects of children with congenital cytomegalovirus infection"Congenital Anomalies. 42. 181-186 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujikawa T, Numazaki K, Asanuma H, Tsutsumi H: "Human cytomegalovirus infection during pregnancy and detection of specific T cells by intracellular cytokine staining"Int J Infect Dis. 7. 215-221 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujikawa T, Numazaki K, Asanuma H, Kudo R, Tsutsumi H: "The frequency of human cytomegalovirus-specific T cells during pregnancy determined by intracellular cytokine staining"J Med Virol. 71. 527-531 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Numazaki K, Umetsu M, Adachi N: "Mycoplasma pneumoniae infection and its genotypical characterization in children of Hokkaido, Japan"In Vivo. 17. 421-424 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Numazaki K: "Glycyrrhizin therapy for viral infections"Afr J Biotechnol. 2. 392-393 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 沼崎 啓: "今日の診断指針"東京,医学書院. 2092 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Numazaki K, Fujikawa T.: "Prevalence of serum antibodies to cytomegalovirus in pregnant women in Sapporo, Japan"Int J Infect Dis. 6:. 149-148 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Numazaki K, Fujikawa T, Asanuma H: "Immunological evaluation and clinical aspects of children with congenital cytomegalovirus infection"Congenital Anomalies. 42:. 181-186 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Numazaki K, Chiba S, Umetsu M, et al.: "Etiological agents of lower respiratory tract infections in Japanese children"Int J Infect Dis. 7:(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujikawa T, Numazaki K, Asanuma H, Tsutsumi H: "Human cytomegalovirus infection during pregnancy and detection of specific T cells by intracellular cytokine staining"Int J Infect Dis. 7:(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Numazaki K, Fujikawa T: "Intracranial calcification with congenital rubella syndrome in a mother with serologic immunity"J. Child. Neurol. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujikawa T, Numazaki K, Asanuma H, et al.: "The frequency of human cytomegalovfrus-specific T cells during pregnancy determined by intracellular cytokine staining"J. Med. Virol.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Numazaki K, Asanuma H, Niida Y: "The Third Symposium on Chlamydial Infections, Proceedings of the Third Joint Auspices of Japan Society for Chlamydia Research and Department of Pathobiology, University of Washington"Life Science Meclica Co. Ltd., Tokyo(in press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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