| Project/Area Number |
14570807
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Kobe University |
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Principal Investigator |
UEDA Masato Kobe University, Dermatology, Associate Professor, 大学院・医学系研究科, 助教授 (20176598)
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| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | UV-carcinogenesis / tumor promotor / cyclooxygenasa-2 / free radical / EGF-receptor / oxidized diacylglycerol / telomerase / apoptosis / ゲニステイン |
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| Research Abstract |
(1)Cyclooxygenase-2 (COX-2) has been shown to be activated by UV and has been suggested to act as a tumor promotor. We examined the mechanism of COX-2 activation by UVB in HaCaT keratinocytes and found that oxygen radicals and activation of EGF-receptor are involved in this event. (2)To reveal that oxidized diacylglycerol acts as tumor promotor of UV-carcinogenesis, oxidized diacylglycerol was applied to the mouse skin after the initiation with DMBA. However, we could not observe the tumor formation. (3)Telomerase activation is the critical step for carcinogenesis and the inhibition of telomerase may lead to suppression of carcinogenesis. The mechanism of telomerase activation in squamous cell carcinome cells was examined and we found that the EGF-receptor activity is important for the telomerase activity. (3)Induction of apoptosis is crucial for the treatment of cancer. Genistein, an isoflavon in soy beans, was examined whether it augments apoptosis in melanoma cells by cisplatinum. Treatment with genistein and cisplatinum increased apoptosis in association with the decreased expression of bcl-2 and bcl-xL and increased expression of Apaf-1.
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