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Molecular cloning of genes expressed downstream the p51 pathway in melanocytes and keratinocytes

Research Project

Project/Area Number 14570816
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionSapporo Medical University

Principal Investigator

YAMASHITA Toshiharu  Sapporo Medical University, Associate Professor, 医学部, 助教授 (50167706)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsp51(p63) / Apoptosis / Transcriptional regulation / Keratinocyte / Melanocyte / Differential display / Micro array / cDNA / CDNA
Research Abstract

One of the p53 family members, p51(p63,p73L or Ket) has been reported to be essential for formation of cutaneous organ and adnexal tissues. By using recombinant adenoviruses that express one of the p53 family proteins, suppression of growth and induction of apoptosis were studied in melanoma cell lines. We found that among p53 family proteins, p51 induced apoptosis most significantly than p53 or p73 in melanoma cells. SK-mel-118 melanoma cells infected with p51-expressing adenovirus(Ad-p51) showed fragmented cellular DNA and activated caspase-3 characteristic of apoptosis. We prepared RNA from Ad-LacZ(control)-and Ad-p51-infected SK-mel-118 cells and compared amount of RNA by the methods of differential-display(DD)-PCR and DNA microarray. As a result, we identified more than twenty cellular genes that were clearly induced by p51. Inducibility of the candidate genes were examined in primary fibroblasts, keratinocytes and melanocytes by RT-PCR. Among them, three genes were induced in keratinocytes but not in fibroblasts or melanocytes. From these results, it is suggested that multiple genes including our candidates are induced by p51 and may have some roles in the process of cutaneous formation.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Hirosaki K, Yamashita T et al.: "Trosinase and tyrosinase related protein-1 (TRP-1) require Rab7 for their intracellular transport."J Invest Dermatol. 119. 475-480 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sasaki Y, Ymashita T et al.: "The p53 family member genes are involved in the notch signal pathway."J Biol Chem. 277. 719-724 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] King KE, Yamashita T et al.: "deltaNp63 functions as both a positive and negative transcriptional regulation and blocks in vitro differentiation of murine keratinocytes."Oncogene. 22. 3635-3644 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jin H-Y, Yamashita T et al.: "Detection of tyrosinase and tyrosinase-related protein 1 sequences from peripheral blood of melanoma patient using reverse transcription-polymerase chain reaction."J Dermatol Science. 33. 169-176 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sasaki Y, Yamashita T et al.: "Identification of the interleukin-4 receptorα gene as a direct target for p73."Cancer Res. 63. 8145-8152 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Satoh A, Yamashita T et al.: "Epigenetic inactivation of CHFR and sensitivity to microtubule inhibitors in gastric cancer."Cancer Res. 63. 8606-8613 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirosaki K, Yamashita T, Wada T, Jin H-Y, Jimbow K: "Tyrosinase and tyrosinase related protein-1(TRP-1) require Rab7 for their intracellular transport."J Invest Dermatol. 119. 475-480 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi M, Sato T, Segawa T, Lu Y, Sato Y, lyama S, Yamada Y, Fukuda J, Fukaura J, Takahashi S, Miyanishi K, Yamashita T, Sasaki K, Kogawa K, Hamada H, Kato J, Niitsu Y: "E1B-55K-deleted adenovirus expressing E1A-13S by AFP-enhancer/promoter is capable of highly specific replication in AFP-producing hepatocellular carcinoma and eradication of established tumor."Mol Ther. 5. 627-634 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sasaki Y, Ishida S, Morimoto I, Yamashita T, Kojima T, Kihara C, Tanaka T, Imai K, Nakamura Y, Tokino T.: "The p53 family member genes are involved in the notch signal pathway."J Biol Chem. 277. 719-724 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Endo M, Yamashita T, Jin H-Y, Akutsu Y, Jimbow K: "Detection of human papilloma-virus type 16 in bowenoid papulosis and nonbowenoid tissues."Int J Dermatol. 42. 474-476 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawakami A, Nakayama H, Yamada Y, Hirosaki K, Yamashita T, Kondo S, Jimbow K: "Dextromethorphan induces multifocal fixed drug eruption."Int J Dermatol. 42. 474-476 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] King KE, Ponnamperuma RM, Yamashita T, Tokino T, Lee LA, Young MF, Weinberg WC: "deltaNp63 functions as both a positive and negative transcriptional regulation and blocks in vitro differentiation of murine keratinocytes."Oncogene. 22. 3635-3644 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jin H-Y, Yamashita T, Minamitsuji Y, Omori F, Jimbow K: "Detection of tyrosinase and tyrosinase-related protein 1 sequences from peripheral blood of melanoma patient using reverse transcription-polymerase chain reaction."J Dermatol Science. 33. 169-176 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sasaki Y, Mita H, Toyota M, Ishida S, Morimoto I, Yamashita T, Tanaka T, Imai K, Nakamura Y, Tokino T: "Identification of the interleukin-4 receptora gene as a direct target for p73."Cancer Res. 63. 8145-8152 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Satoh A, Toyota M, Itoh F, Sasaki Y, Suzuki H, Ogi K, Kikuchi T, Mita H, Yamashita T, Kojima T, Kusano M, Fujita M. Hosokawa M, Endo T, Tokino T, Imai K: "Epigenetic inactivation of CHFR and sensitivity to microtubule inhibitors in gastric cancer."Cancer Res. 63. 8606-8613 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ono I, Yamashita T, Hida T, Jin H-Y, Ito Y, Hamada H, Akasaka Y, Ishii T, Jimbow K: "Combined administration of basic fibroblast growth factor protein and the hepatocyte growth factor gene enhances the regeneration of dermis in acute incisional wounds."Wound Rep Reg. 12. 67-79 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ono I, Yamashita T, Hida T, Jin H-Y, Ito Y, Hamada H, Akasaka Y, Ishii T, Jimbow K: "Local administration of hepatocyte growth factor(HGF) gene enhances the regeneration of dermis in acute incisional wounds."J Surg Res. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jin H-Y, Yamashita T, et al.: "Detection of tyrosinase and tyrosinase-related protein 1 sequences from peripheral blood of melanoma patients using reverse transcription-polymerase chain reaction"J Dermatol Science. 33. 169-176 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sasaki Y, Yamashita T, et al.: "Identification of the interleukin-4 receptor gene as a direct target for p73"Cancer Res. 63. 8145-8152 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Satoh A, Yamashita T, et al.: "Epigenetic inactivation of CHFR and sensitivity to microtubule inhibitors in gastric cancer"Cancer Res. (in press). (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] King KE, Yamashita T, et al.: "deltaNp63 functions as both a positive and negative transcriptional regulation and blocks in vitro differentiation of murine keratinocytes"Oncogene. 22. 3635-3644 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirosaki K, Yamashita T, et al.: "Tyrosinase and TRP-1 require Rab7 for the Intracellular Transport from TGN to melanosomes, passing through early and late endosomes"J Invest Dermatol. 119. 475-480 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sasaki Y, Yamashita T, et al.: "The p53 family member genes are involved in the notch signal pathway"J Biol Chem. 277. 719-724 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] King KE et al.: "DelNp63 functions as a positive and negative transcriptional regulator and blocks in vitro differentiation of murine keratinocytes"Oncogene. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Endo M et al.: "Detection of human papillomavirus type 16 in bowenoid papulosis and nonbowenoid tissues"Int J Dermatol. (in press). (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirosaki K et al.: "Tyrosinase and tyrosinase related protein-1 (TRP-1) require Rab7 for their intracellular Transport"J Invest Dermatol. 119・2. 475-480 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takahashi M et al.: "E1B-55K-deleted adenovirus expressing E1A-13S by AFP-enhancer/promoter is capable of highly specific replication in AFP-producing hepatocellular carcinoma and eradication of established tumor"Molecular Therapy. 5・5. 627-634 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sasaki Y et al.: "The p53 family member genes are involved in the notch signal pathway"J Biol Chem. 277・1. 719-724 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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