| Project/Area Number |
14570820
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Dokkyo Medical University |
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Principal Investigator |
OHTSUKA Thutomu Dokkyo University, School of Medicine, MD, Associate Professor, 医学部, 助教授 (10241879)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | systemic sclerosis / immunoglobulin E / class switch / direct switch / sequential switch / Immunodobulin E / IgE |
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| Research Abstract |
Background : The involvement of mast cell, which is activated by immunoglobulin E (IgE), has been reported in the formation of SSc abnormality. IgE is generated with isotype switch. During isotype switch, switch circles resulting from direct μ-ε or front sequential μ-γ1-ε switching will be created.
Objective : We studied whether switching occurs in SSc.
Methods : We used nested polymerase chain reaction to analyze the S fragments from switch circles. Fifty-two patients with SSc. and 62 healthy women were studied.
Results : None of 62 normal skin tissues showed direct switch nor sequential switch. None of 7 normal whole blood cells showed direct switch nor sequential switch. In 52 SSc skin tissues. 3 (3.8%) showed direct switch. and 2 (3.8%) showed sequential switch. As a result, 5 (9.6%) of SSc skin tissue showed immunoglobulin E class switch. These results were continued by DNA sequencing.
Conclusion : These results demonstrated that isotype switch to the c locus achieved by direct and/or sequential switch arc involved in SSc skin.
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