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Genome expression profiling of heavy ion irradiation for head and neck cancer using in-house DNA chips.

Research Project

Project/Area Number 14570897
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionNational Institute of Radiological Sciences

Principal Investigator

MIZOE Jyunetsu  National Institute of Radiological Sciences, Research Center for Charged Particle Therapy, The chief of hospital, 重粒子医科学センター, 研究員 (80091510)

Co-Investigator(Kenkyū-buntansha) TSUJII Hirohiko  National Institute of Radiological Sciences, Research Center for Charged Particle Therapy, Director, 重粒子医科学センター, 研究員 (50088853)
TANZAWA Hideki  Chiba University, Clinical Molecular Biology, Professor, 大学院・医学研究院, 教授 (50236775)
SHIBAHARA Takahiko  Tokyo Dental College, Department of oral and maxillofacial surgery, Professor, 歯学部, 教授 (50178919)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsCharged Particle Therapy / RNA manifestation / cDNA tip / Gene response / Head and Neck Cancer / malignant melanoma / Adenocarcinoma / Squamous cell carcinoma / 頭頚部腫瘍
Research Abstract

Cultured cells of Ca9-22 (originating from squamous cell carcinoma of the gum), G361 (malignant melanoma of the skin), HSG (adenocarcinoma of the salivary gland) and HaCaT (keratinocyte of the skin) were irradiated with x-ray, carbon ions and neon ions. The post-irradiation cell survival rate for each cell lines was determined by the colony assay and a dead cell count taken by the dye exclusion method. The D_<10> and SF_4 show in the order from Ca9-22<G361<HSG<HaCaT in x-ray and G361<HSG<Ca9-22<HaCaT in carbon and neon ions. These findings confirmed that x-ray resistant G361 cells and HSG cells are well controlled by carbon and neon ions.
Corresponding genes of x-ray and carbon ions irradiation were identified by gene expression analysis (gene chip U133A). The Ca9-22 cells were irradiated with x-ray and carbon ions, and gene expression analysis was performed four hours after irradiation. The number of up regulated genes was 144 after x-ray and 286 after carbon ions. The number of down regulated genes was 402 in x-ray and 331 in carbon ions.
Similarly, G361 cells were irradiated with carbon ions. The number of up regulated genes was 85 and the number of down regulated genes was 111. Comparison of gene expression between Ca9-22 cells and G361 cells shows that 6 genes exhibited an up regulation commonly while six genes revealed a down regulation commonly after both irradiation. From the results of clinical research, malignant melanoma have a higher local control rate by carbon ion radiotherapy than squamous cell carcinoma. From investigations of the genes that are expressed in a specific or consistent manner in either of these cell lines, it was possible to estimate the group of carbon ion sensitive genes and the group of carbon ion resistant genes, respectively. This evidence suggests that certain already known gene groups such as CyclinD1, in particular, determine the differences in the effectiveness of heavy particle radiation.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (8 results)

All 2004 2003 Other

All Journal Article (6 results) Publications (2 results)

  • [Journal Article] Dose escalation study of carbon ion radiotherapy for locally advanced head-and-neck cancer.2004

    • Author(s)
      JUN-ETSU MIZOE, HIROHIKO TSUJII, TADASHI KAMADA, YOSHISUKE MATSUOKA, HIROSHI TSUJI, YASUHIRO OSAKA, AZUSA HASEGAWA, NOBUHARU YAMAMOTO, SATOSHI EBIHARA, AKIYOSHI KONNO
    • Journal Title

      Int.J.Radiation Oncology Biol.Phys. 60

      Pages: 358-364

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 口腔扁平上皮癌由来細胞株を用いた放射線および重粒子線照射による遺伝子発現プロファイル2003

    • Author(s)
      溝江純悦, 山本信治, 神津由直, 肥後盛洋, 加藤真樹, 関 直彦, 瀧口正樹
    • Journal Title

      第21回日本口腔腫瘍学会総会・学術大会

      Pages: 97-97

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 悪性腫瘍と正常上皮における重粒子線によるDNA構造変化の検討2003

    • Author(s)
      丹沢秀樹, 溝江純悦, 鵜澤一弘, 椎葉正史, 柴原孝彦, 山本信治
    • Journal Title

      平成14年度放射線医学総合研究所 重粒子線がん治療装置等共同利用研究報告書

      Pages: 170-171

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] In-house cDNA microarray analysis of gene expression profiles involved in SCC cell lines.2003

    • Author(s)
      Moriya T, Seki N, Shimada K, Kato M, Yakushiji T, Nimura Y, Uzawa K, Takiguchi M, Tanzawa H.
    • Journal Title

      Int J Mol Med. 12

      Pages: 429-435

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Genome expression profiling of X-ray and heavy ions in cell lines of oral squamous cell carcinoma.2003

    • Author(s)
      Junetsu Mizoe, Nobuharu Yamamoto, Yukinao Kouzu, Morihiro Higo, Masaki Kato, Naohiko Seki, Masaki Takiguchi
    • Journal Title

      The 21^<th> General Meeting Japan society of Oral Tumours 97

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Alternation of DNA structures after heavy ion irradiation for malignant tumor and normal epithelium.2003

    • Author(s)
      Hideki Tanzawa, Junetsu Mizoe, Katsuhiro Uzawa, Masashi Shiiba, Takahiko Shibahara, Nobuharu Yamamoto
    • Journal Title

      Annual report of HIMAC

      Pages: 170-171

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 溝江純悦, 山本信治, 神津由直, 肥後盛洋, 加藤真樹, 関 直彦, 瀧口正樹: "口腔扁平上皮癌由来細胞株を用いた放射線および重粒子線照射による遺伝子発現プロファイル"第21回日本口腔腫瘍学会総会・学術大会. 97 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 丹沢秀樹, 溝江純悦, 鵜澤一弘, 椎葉正史, 柴原孝彦, 山本信治: "悪性腫瘍と正常上皮における重粒子線によるDNA構造変化の検討"平成14年度放射線医学総合研究所 重粒子線がん治療装置等共同利用研究報告書. 170-171 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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