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Involvement of reward system in acquisition and maintenance of alcohol independence.

Research Project

Project/Area Number 14570928
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionKagawa University

Principal Investigator

TAKEUCHI Yoshiki  Kagawa University, Faculty of Medicine, Professor, 医学部, 教授 (20116619)

Co-Investigator(Kenkyū-buntansha) MIKI Takanori  Kagawa University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (30274294)
YOKOYAMA Toshihumi  Kagawa University, Faculty of Medicine, Assistant Professor, 医学部, 助手 (10380156)
WATANABE Takemi  Kagawa University, University Hospital, Assistant Professor, 医学部附属病院, 助手 (50304598)
松本 由樹  香川大学, 医学部, 助手 (90335844)
宮武 良輔  香川医科大学, 医学部附属病院, 助手 (50301320)
Project Period (FY) 2002 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsAlcohol / Dependence / Model animal / Rewards system / Hippocampus / Glial cells / Amino acids / Neurotrophic factors / アルコー依存症 / 脳内報酬系 / BDNF mRNA / GDNF mRNA / OMgp mRNA / Real-time RT-PCR / アルコール依存症 / 脳内アミノ酸 / 神経伝達物質 / 微細構造変化 / 神経細胞数の定量解析 / 海馬神経細胞 / 種差 / 脆弱性 / 血管内皮 / タウリン / 保護 / 修復作用
Research Abstract

Wistar rats were exposed to a relatively high daily dose of ethanol at postnatal day 10-15 by placing them for 3 h/day in a chamber containing ethanol vapor. These ethanol-treated rats (blood concentration : approximately 430 mg/dl) were found to have slightly but significantly fewer pyramidal cell neurons. The present observations indicate that pyramidal cells in the hippocampus may be vulnerable to a relatively high dose of ethanol exposure during this short period of early postnatal life. In additional experiment, the effects on the frontal cortex and hilar neuron numbers, observed as a result of such short periods of ethanol treatment, appeared to be transitory.
With respect to colocalization of taurine and glial fibrillary acidic protein immunoreactivity in mouse hippocampus, induced by short-term ethanol exposure, quantitative analysis revealed that the merged area in ethanol-treated mice was twice that (71.6% vs. 35.8%) of control mice. Since taurine is involved in various neuroprotective functions, the present observations suggest that the expression of taurine immunoreactivity in reactive astrocytes after ethanol exposure might play an important role in neuroprotective processes.
Additional analysis was made to investigate the effects on expression of neurotrophic factors. The expression levels of brain- and glia-derived neurotrophic factors (BDNF and GDNF) in the hippocampus were analyzed by RT-PCR analysis. This study revealed that chronic ethanol consumption does not result in significant changes in expression levels of BDNF and GDNF mRNAs. However, it was characteristic in myelin-associated glycoprotein that expression levels of oligodendrocyte myelin glycoprotein (OMgp) mRNA were increased. This appears to be consistent with the neuropathological changes in alcoholics, which have relationship with demyelination.

Report

(5 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (36 results)

All 2006 2005 2004 2003 2002 Other

All Journal Article (27 results) Publications (9 results)

  • [Journal Article] Effects of Chronic Ethanol Administration on the Expression Levels of Neurotrophic Factors in the Rat Hippocampus.2006

    • Author(s)
      Okamot et al.
    • Journal Title

      Okajimas Folia Anat Jpn (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regional differences in vulnerability of the cerebellar foliations of rats exposed to neonatal X-irradiation.2006

    • Author(s)
      Li et al.
    • Journal Title

      Neurosci Lett 402

      Pages: 86-91

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of chronic ethanol administration on the expression levels of neurotrophic factors in the rat hippocampus.2006

    • Author(s)
      Okamot et al.
    • Journal Title

      Okajimas Folia Anat Jpn (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of Chronic Ethanol Administration on the Expression Levels of Neurotrophic Factors in the Rat Hippocampus2006

    • Author(s)
      Okamot et al.
    • Journal Title

      Okajimas Folia Anatomica Japonica (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Changes of parvalbumin lmmunoreactlve neurons and GFAP immunoreactive astrocytes in the rat lateral geniculate nucleus following monocular enucleation.2006

    • Author(s)
      Gonzalez D et al.
    • Journal Title

      Neuroscience Letters 395(2)

      Pages: 149-154

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Application of the physical disector to central nervous system : estimation of the total number of neurons in the subdivisions of the rat hippocampus.2005

    • Author(s)
      Miki et al.
    • Journal Title

      Anat Sci Int 80

      Pages: 153-162

    • NAID

      10020556930

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Multiple defects in the formation of rat cortical axonal pathways following prenatal X-ray irradiation.2005

    • Author(s)
      Li et al.
    • Journal Title

      Eur J Neurosci 21

      Pages: 1847-1858

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Application of the physical disector to central nervous system estimation of the total number of neurons in the subdivisions of the rat hippocampus2005

    • Author(s)
      Miki et al.
    • Journal Title

      Anatomical Science International 80

      Pages: 153-162

    • NAID

      10020556930

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of monocular enucleation on calbindin-D 28k and c-Fos expression in the lateral geniculate nucleus in rats2005

    • Author(s)
      Gonzalez D et al.
    • Journal Title

      Okajimas Folia Anatomica Japonica 82

      Pages: 1-18

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Multiple defects in the formation of rat cortical axonal pathways following prenatal X一ray irradiation2005

    • Author(s)
      Li HP et al.
    • Journal Title

      European Journal of Neuroscience 21

      Pages: 1847-1858

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Parabrachial inputs to Fos-immunoreactive neurons in the lateral central nucleus of amygdala : a light and electron microscopic study in the rat.2004

    • Author(s)
      Takeuchi et al.
    • Journal Title

      Brain Res Bull 64

      Pages: 171-180

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of age and alcohol exposure during early life on pyramidal cell numbers in the CA1-CA3 region of the rat hippocampus.2004

    • Author(s)
      Miki et al.
    • Journal Title

      Hippocampus 14

      Pages: 124-134

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Kindling of the ventral tegmental area induces supersensitivity in the central dopamine system.2004

    • Author(s)
      Watanabe et al.
    • Journal Title

      Brain Res 1003

      Pages: 194-198

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional polymorphisms in the sigma receptor gene associated with alcoholism.2004

    • Author(s)
      Miyatake et al.
    • Journal Title

      Biol Psychiat 55

      Pages: 85-90

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional polymorphisms in the sigma, receptor gene associated with alcoholism.2004

    • Author(s)
      Miyatake et al.
    • Journal Title

      Biol Psychiat 55

      Pages: 85-90

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Parabrachial inputs to Fos-immunoreactive neurons in the lateral central nucleus of amygdala activated by hypotension : a light and electron microscopic study in the rat.2004

    • Author(s)
      Takeuchi et al.
    • Journal Title

      Brain Res Bull. 64

      Pages: 171-180

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of age and alcohol exposure during early life on pyramidal cell numbers in the CA1-CA3 region of the rat hippocampus.2004

    • Author(s)
      Miki et al.
    • Journal Title

      HPPOCAMPUS 14

      Pages: 124-134

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Kindling of the ventral tegmental area induces supersensitivity in the central dopamine system2004

    • Author(s)
      Watanabe et al.
    • Journal Title

      Brain Res 1003

      Pages: 194-198

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Excessive testosterone treatment and castration induce reactive astrocytes and fos immunoreactivity in suprachiasmatic nucleus of mice.2004

    • Author(s)
      Satriotomo et al.
    • Journal Title

      Brain Res 1020

      Pages: 130-139

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Enhancement of central dopaminergic activity in the kainate model of temporal lobe epilepsy : implication for the mechanism of epileptic psychosis.2004

    • Author(s)
      Ando et al.
    • Journal Title

      Neuropsychopharmacology 29

      Pages: 1251-1258

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Quantitative evaluation of central-type benzodiazepine receptors with [(125)I]Iomazenil in experimental epileptogenesis : II. The rat cortical dysplasia model.2004

    • Author(s)
      Morimoto et al.
    • Journal Title

      Epilepsy Res 61

      Pages: 113-118

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Colocalization of taurine and glial fibrillary acidic protein immunoreactivity in mouse hippocampus induced by short-term ethanol exposure.2003

    • Author(s)
      Sakurai et al.
    • Journal Title

      Brain Res 959

      Pages: 160-164

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The effects of alcohol exposure during early life on neuron numbers in the rat hippocampus : I)hilus neurons and granule cells.2003

    • Author(s)
      Miki et al.
    • Journal Title

      Hippocampus 13

      Pages: 388-398

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The effects of alcohol exposure during early life onneuron numbers in the rat hippocampus : I)hilus neurons and granule cells.2003

    • Author(s)
      Miki et al.
    • Journal Title

      Hippocampus 13

      Pages: 388-398

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] the effect of the timing of prenatal X-irradiation on Purkinje cell numbers in rat cerebellum.2002

    • Author(s)
      Li et al.
    • Journal Title

      Dev Brain Res 139

      Pages: 159-166

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Delusional disorder : molecular genetic evidence for dopamine psychosis.2002

    • Author(s)
      Morimoto et al.
    • Journal Title

      Neuropsychopharmacology 26

      Pages: 794-801

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The effect of the timing of prenatal X-irradiation on Purkinje cell numbers in rat cerebellum.2002

    • Author(s)
      Li et al.
    • Journal Title

      Dev Brain Res 139

      Pages: 159-166

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Takanori Miki: "Effects of age and alcohol exposure during early life on pyramidal cell numbers in the CA1-CA3 region of the rat hippocampus"Hippocampus. 14. 124-134 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Gu He: "Toluene diisocyanate exposure induces laryngo-tracheal eosinophilia, which canbe ameliorated by supplementation with antioxidant vitamins in guinea pigs"Acta Otolaryngol. 123. 965-971 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takemi Watanabe: "Kindling of the ventral tegmental area induces supersensitivity in the central dopamine system"Brain Res. 1003. 194-198 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kiyoshi Morimoto: "Delusional disorder : molecular genetic evidence for dopamine psychosis."Neuropsychopharmacology. 26. 794-801 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hong-Peng Li: "The effect of the timing of prenatal X-irradiation on Purkinje cell numbers in rat cerebellum"Dev Brain Res. 139・2. 159-166 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takanobu Sakurai: "Colocalization of taurine and glial fibrillary acidic protein immunoreactivity in mouse hippocampus induced by short-term ethanol exposure"Brain Res. 959・1. 160-164 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takanori Miki: "The effects of alcohol exposure during early life on neuron numbers in the rat hippocampus : I)hilus neurons and granule cells"Hippocampus. 13. 388-398 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takanori Miki: "The effects of age and alcohol exposure during early life on pyramidal cells in the CA1-CA3 region of the rat hippocampus"Hippocampus. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kiyoshi Morimoto: "Delusional disorder : molecular genetic evidence for dopamine psychosis"Neuropsychopharmacology. 26・6. 794-801 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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