• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The development of culture system for platelet substitute production using human hematopoietic stem cells and megakaryocytic cell line

Research Project

Project/Area Number 14571000
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionSaitama Medical University (2003)
Tokyo Medical University (2002)

Principal Investigator

IKEBUCHI Kenji  Saitama Medical University, Department of Transfusion and Cell Therapy, Professor, 医学部, 教授 (20175194)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsPlatelet / Substitute / Megakaryocytic line / Bone marrow stroma / human cord blood / CD34+ cell / SCIDマウス / 造血幹細胞 / 増幅 / ストロマ細胞 / 化学固定 / 巨核球
Research Abstract

Human cord blood CD34+ cells produced approximately 1000-fold hematopoietic cells and 100-fold CD34+ cells in the culture system consisting of TPO, FL, SCF and human bone marrow-derived stroma cells. The function of stroma cells required cell-cell direct contact and this capability was partially preserved after chemical fixation.
Cord blood C34+ cells differentiated to megakaryocytic cells after culture in the presence of TPO and SCF, however, massive expansion was not obtained. In addition, the terminal maturation such as proplatelet formation, was not observed. Human megakaryocytic cell line(Meg-J) cells produced platelet-like particles in the culture. The addition of TPA or protein kinase inhibitor promoted cell maturation, together cell apoptosis. Platelet-particles consisted of cellular fragment and apoptotic bodies.
In order to permanently use hematopoietic cytokines in culture, the method to coat cytokines on culture device surface area was examined. Photoreactive gelatin and erythropoietin(EPO) was fixed on the plastic surface by use of UV irradiation. Epo-dependent cell line grew on EPO-fixed surface, but did not grow on negative area, indicating that this coating method was applicable.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report

Research Products

(16 results)

All Other

All Publications

  • [Publications] Ito Y, Hasuda H, Yamauchi T, Komatsu N, Ikebuchi K: "Immobilization of erythropoietin to culture erythropoietin-dependent human leukemia cell line."Biomaterials. 25(12). 2293-2298 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirayama F, Yamaguchi M, Yano M, Yasui K, Horie Y, Matsumoto K, Nagao N, Ikebuchi K, Azuma H, Ikeda H, Tani Y: "Spontaneous and rapid reexpression of functional CXCR4 by human steady-state peripheral blood CD34+ cells"Int J Hematol. 78(1). 48-55 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kuwahara I, Ikebuchi K, Hamada H, Niitsu Y, Miyazawa K, Ohyashiki K, Fujisawa H, Furukawa K: "Changes in N-glycosylation of human stromal cells by telomerase expression."Biochem Biophys Res Commun. 301(2). 293-297 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawano Y, Kobune M, Yamaguchi M, Nakamura K, Ito Y, Sasaki K, Takahashi S, Nakamura T, Chiba H, Sato T, Matsunaga T, Azuma H, Ikebuchi K, Ikeda H, Kato J, Niitsu Y, Hamada H: "Ex vivo expansion of human umbilical cord hematopoietic progenitor cells using a coculture system with human catalytic subunit(hTERT)-transfected human stromal cells."Blood. 101(2). 532-540 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ito Y, Hasuda H, Yamauchi T, Komatsu N, Ikebuchi K: "Immobilization of erythropoietin to culture erythropoietin-dependent human leukemia cell line."Biomaterials. 25. 2293-2298 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirayama F, Yamaguchi M, Yano M, Yasui K, Horie Y, Matsumoto K, Nagao N, Ikebuchi K, Azuma H, Ikeda H: "Spontaneous and rapid reexpression of functional CXCR4 by human steady-state peripheral blood CD34+ cells."Int J Hemaol. 78. 48-55 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kuwahara I, Ikebuchi K, Hamada H, Niitsu Y, Miyazawa K, Ohyashiki K, Fujisawa H, furukawa K: "Changes in N-glycosylation of human stromal cells by telomerase expression."Biochem Biophys Res Commun. 301(2). 293-297 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawano Y, Kobune M, Yamaguchi M, Nakamura K, Ito Y, Sasaki K, Takahashi S, Nakamura T, Chiba H, Sato T, Matsunaga T, Azuma H, Ikebuchi K, Ikeda H, Kato J, Niitsu Y, Hamada H: "Ex vivo expansion of human umbilical cord hematopoietic progenitor cells using a coculture system with human telomerase catalytic subunit(hTERT)-transfected human stromal cells."Blood. 101(2). 532-540 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ito Y, Hasuda H, Yamauchi T, Komatsu N, Ikebuchi K: "Immobilization of erythropoietin to culture erythropoietin-dependent human leukemia cell line."Biomaterials. 25(12). 2293-2298 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirayama F, Yamaguchi M, Yano M, Yasui K, Horie Y, Matsumoto K, Nagao N, Ikebuchi K, Azuma H, Ikeda H, Tani Y: "Spontaneous and rapid reexpression of functional CXCR4 by human steady-state peripheral blood CD34+cells."Int J Hematol. 78(1). 48-55 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kuwahara I, Ikebuchi K, Hamada H, Niitsu Y, Miyazawa K, Ohyashiki K, Fujisawa_H, _Furukawa_K: "Changes in N-glycosylation of human stromal cells by telomerase Expression."Biochem Biophys Res Commun. 301(2). 293-297 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawano Y, Kobune M, Yamaguchi M, Nakamura K, Ito Y, Sasaki K, Takahashi S, Nakamura T, Chiba H, Sato T, Matsunaga T, Azuma H, Ikebuchi K, Ikeda H, Kato J, Niitsu Y, Hamada H: "Ex vivo expansion of human umbilical cord hematopoietic progenitor cells using a coculture system with human telomerase catalytic subunit(hTERT)-transfected human stromal cells."Blood. 101(2). 532-540 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 池淵研二: "Changes in N-glycosylation of human stromal cells by telomerase expression"Biochem Biophys Res Commun. 301. 293-297 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 池淵研二: "Ex vivo expansion of human umbilical cord hematopoietic progenitor cells using a coculture system with human telomerase catalytic subunit (h-TERT)transfected"Blood. 101. 532-540 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 池淵研二: "Bone marrow stromal cells prepared using AB serum and bFGF for hematopoietic stem cell expansion"Transfusion. 42. 921-927 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 池淵研二: "Ex vivo expansion of human UC blood primitive hematopoietic progenitor and transplantable stem cells using human primary BM stromal cells and human AB serum"Cytotherapy. 4. 109-118 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi