| Project/Area Number |
14571006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | RIKEN (The Institute of Physical and Chemical Research) |
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Principal Investigator |
ERA Takumi RIKEN (The Institute of Physical and Chemical Research), Center for Developmental Biology, Stem cell biology group, researcher, 幹細胞研究グループ, 研究員 (00273706)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | ES cell / MLL gene / tetracycline gene regulation / MLL-AF9 / Hox gene / leukemia / model system / in vitro differentiation system / paraxial mesoderm / lateral mesoderm / FLK1 / PDGFRα |
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| Research Abstract |
ES cell is pluripotent cell line which gives rise to various cell lineages in vitro and in vivo. When cultured in the presence of LIF and serum, ES cell undergo unlimited symmetrical self-renewal divisions. It provides us a reseanable cell number to analyze the molecular processes of development in vitro. The forced differentiation of ES cell in vitro can be utilized as a good tool to assess the developmental questions. However, in vitro ES cell differentiation system is still very complex, in which various cell types are generated simultaneously. This is one of major reason why in vitro ES cell system is not so popular. In spite of these difficulties, ES cell system is still attractive system and posses many advantage when comparing to other methods. We have utilized in vitro ES cell differentiation system to understand the specification and diversification of mesoderm and blood progenitors development.
Classically, mesoderm cells segregate from primitive streak and diversified into three major population including paraxial, lateral and intermediate type. Early gastrula organizer and node can contribute to axial mesoderm and gut endoderm. Therefore, the organizer and their derivatives can be called as mesendoderm precursors that give rise to mesoderm and endoderm. We have attempted to establish the define condition to control ES cell toward some specific mesoderm lineage. One of good markers for early organigzer is Goosecoid (Gsc). In an early mouse development, Gsc is expressed in the anterior end of primitive streak and the expression is observed in axial mesoderm and gut at later stage. Recently, we have established defined culture condition to control ES cell to mesendoderm precursors expressing Gsc. These finding is useful for analyzing Hox-related leukemias.
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