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Generation of an anti-fibrotic therapeutic gene using a tubular epithelium-specific expression gene casette and iKB cDNA

Research Project

Project/Area Number 14571033
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionSaitama Medical School, School of Medicine

Principal Investigator

KANNO Yoshihiko  Saitama Medical School, Department of Nephrology, Assistant Professor, 医学部, 講師 (30276232)

Co-Investigator(Kenkyū-buntansha) OKADA Hirokazu  Saitama Medical School, Department of Nephrology, Associate Professor, 医学部, 助教授 (60233342)
KIKUTA Tomohiro  Saitama Medical School, Department of Nephrology, Postdoctoral fellow, 医学部, 助手 (70383239)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
KeywordsOsteopontin / promoter analysis / gene transfection / promoter / オステオポンチン / 遺伝子導入 / プロモーター解析 / iKB
Research Abstract

To identify tubular epithelium-specific, positive regulatory elements in the promoter of osteopontin (OPN) gene, we generated luciferase reporter gene constructs bearing various fragments of the OPN promoter and performed transient transfection experiments using murine tubular epithelial cells (mPTEC). In addition to mPTEC, fibroblasts (3T3) and monocytes (RAW264) were also employed for the transfection experiments. The -600 to -825 bp fragment of the OPN promoter constitutively showed positive transcription activity in mPTEC, but not in 3T3 and RAW264. In contrast, the -115 to -189 bp fragment showed positive transcription activity in mPTEC as well as 3T3 and RAW264 in response to recombinant transforming growth factor-b1 treatment. Thus, the -600 to -825 bp fragment is suggested to contain tubular epithelium-specific, positive regulatory elements. We are now generating tubular epithelium-specific expression gene casette using this fragment, and testing whether tubular epithelium-specific expression of iKB can attenuate fibrosis through down-regulation of NF-kB activity in vitro and in vivo.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (1 results)

All 2003

All Journal Article (1 results)

  • [Journal Article] Direct contact between human peripheral blood mononuclear cells and renal fibroblasts facilitates the expression of monocyte chemoattractant protein-12003

    • Author(s)
      Hao, L., H.Okada, Y.Kanno, T.Inoue, T.Kobayashi, Y.Watanabe, et al.
    • Journal Title

      Am J Nephrol 23・4

      Pages: 208-213

    • Related Report
      2004 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

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