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Effect of a single nucleotide polymorphism of complement decay accelerating factor (DAF) on proteinuria in BUF/Mna rat

Research Project

Project/Area Number 14571037
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionShowa University

Principal Investigator

MORITA Hiroyuki  Showa University, Department of Medicine, Associate Professor, 医学部, 講師 (00311994)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsrat experimental nephrology / proteinuria / focal and segmental glomerulosclerosis / disease susceptible gene / QTL analysis / SNP / 疾患発症感受性遺伝子 / 一塩基多型 / ラット
Research Abstract

BUF/Mna strain spontaneously develops proteinuria due to focal and segmental glomeruloscierosis (FSGS). Fine quantitative trait locus (QTL) mapping and synteny analysis clarified that rat homologue of DAF (Daf) located on proteinuria susceptible locus, designated as Purl locus, of rat chromosome 13. A single nucleotide polymorphism in the coding sequence (cSNP) with C-T substitution was found at the nucleotide position 239 of the Daf cDNA in BUF/Mna strain. DNA was obtained in 167 [BUF/Mna x WKYINCrj] F1 x BUF/Mna rats. Urinary protein excretion of these rats were measured. However, analysis of correlation between cSNP and amount of urinary protein excretion has not been completed within the term of the present project. On the other hand, a congenic strain was established in which Purl region of BUF/Mna strain containing Daf was transferred to the genetic background of WKY/NCrj strain. Unilateral nephrectomy was performed in the congenic rats (N=7) and WKY/NCrj rats (N=7) used as controls. At 3 and 9 months, glomerulosclerosis score of the congenic rats was significantly greater than that of WKY/NCrj rats. At 3, 6, and 9 months, urinary protein excretion of the congenic rats was significantly greater than that of WKY/NCrj rats. These results confirmed that Purl region containing Daf had biologic impact on the development of FSGS.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Morita H et al.(筆頭): "Collagenofibrotic glomerulopathy with a widespread expression of type-V collagen."Virchows Arch. 442. 163-168 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Rossi M, Morita H, et al.(2番目): "Heparan sulfate chains of perlecan are indispensable in the lens capsule but not in the kidney."EMBO J. 22. 236-245 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakao N, Yoshimura A, Morita H, et al.(3番目): "Combination treatment of angiotensin-II receptor blocker and angiotensin-converting enzyme inhibitor in non-diabetic renal disease (COOPERATE): a randomised controlled trial"Lancet. 361. 117-124 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki R, Morita H, et al.(2番目): "Mixed cryoglobulinemia due to chronic hepatitis C with severe pulmonary involvement"Internal Medicine. 42. 1210-1214 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morita, H. et al.: "(Ist): Collagenofibrotic lomerulopathywith a widespread expression of type-V collagen."Nirchowas Arch. 442. 163-168 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Rossi, M., Morita, H.et al.: "(2nd); Heparan sulfate chains of perlecan are indispensable in the lens capsule but not in the kidney."EMBO J. 22. 236-245 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakao, N, Yoshimura, A., Morita, H. et al.: "(3rd): Combination treatment of angiotensin-II receptor blocker and angiotensin-converting enzyme inhibitor in non-diabetic renal disease (COOPERATE): a randomized controlled trial."LANCET. 361. 117-124 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki, R., Morita, H.et al.: "(2nd): Mixed cryoglobulinemia due to chronic hepatitis C with severe pulmonary involvement."Internal Medicine. 42. 1210-1214 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morita H at al.: "Collagenofibrotic glomerulopathy with a widespread expression of type-V collagen."Virchows Arch. 442. 163-168 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Rossi M, Morita H et al.: "Heparan sulfate chains of perlecan are indispensable in the lens capsule but not in the kidney."EMBO J. 22. 236-245 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakao N, Yoshimura A, Morita H et al.: "Combination treatment of angiotensin-II receptor blocker and angiotensin-converting-enzyme inhibitor in non-diabetic renal disease (COOPERATE) : a randomised controlled trial."Lancet. 361. 117-124 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki R, Morita H et al.: "Mixed Cryoglobulinemia due to chronic hepatitis C with severe pulmonary involvement"Internal Medicine. 42. 1210-1214 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Rossi, M., MORITA, H.他: "Heparan sulfate chains of perlecan are indispensable in the lens capsule but not in the kidney"The EMBO Journal. 22・2. 236-245 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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