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Molecular mechanisms controlling pancreatic β cell regeneration and pathophysiological significance of pancreatic nestin-expressing cells

Research Project

Project/Area Number 14571063
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Endocrinology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

HOSODA Kiminori  Kyoto University, Graduate School of Medicine, Assistant Professor, 医学研究科, 講師 (40271598)

Co-Investigator(Kenkyū-buntansha) HAYASHI Tatsuya  Kyoto University, Graduate School of Medicine, Assistant Professor, 医学研究科, 助手 (00314211)
INOUE Gen  Kyoto University, Graduate School of Medicine, Assistant Professor, 医学研究科, 講師 (20260606)
ITO Hiroshi  Kyoto University, Graduate School of Medicine, Assistant Professor, 医学研究科, 助教授 (40252457)
YOSHIMASA Yasunao  National Cardiovascular Center, Division of Atherosclerosis, Metabolism and Clinical Nutrition, Director, 動脈硬化代謝部, 部長 (00252437)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsB cell / regeneration / neurogenin3 / nestin / notch / pancreatic duct cells / 中枢神経系 / 未分化 / 膵臓 / 内胚葉 / Notch / streptozotocin膵β細胞再生モデルラット / 血管内皮細胞 / von Willebrand factor
Research Abstract

(Purpose) Pancreatic β cell is derived from endoderm. Similarities have been pointed out between pancreatic β cells and neuronal cells. In the present study, we investigated the significance of neurogenin 3 (ngn3), nestin, and notch2.
(Methods and Results) NGN3 gene expression was very low in streptozotocin-induced pancreatic β cell regeneration rat model, indicating that ngn3 gene expression is not changed in β cell regeneration. Although ngn3 gene expression was noted in central nervous system of rat embryo, postnatally it was not detected in normal rats and in streptozotocin-induced pancreatic β cell regeneration rat model. Using GFP immunofluorescence and anti-GFP antibody, nestin was expressed in vascular endothelial cells of pancreata of streptozotocin-induced pancreatic β cell regeneration rat model and normal rats. Postnatally, using Notch2-LacZ knock-in mouse and immunohistochemistry of anti-Notch2 antibodies, notch2 is expressed mainly in pancreatic duct cells.
(Discussion) Since ngn3 was not detected in postnatal pancreas, the significance of ngn 3 in postnatal pancreatic regeration remains to be elucidated. In the present study, nestin was demonstrated to be expressed in vascular endothelial cells. Since β cell is derived from endodermal origin, nestin-expressing cells appear to be precursors of pancreatic β cells. Pancreatic duct cells has been proposed to be derived from pancreatic duct cells. Notch2-expressing cells may be precursors of β cells. We are investing the significance of notch2 using pancreas-tissue specifc kock-out mice.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] T.Toyoda, K.Hosoda, et al.: "Possible involvement of the {alpha}1 isoform of 5'AMP-activated protein kinase in oxidative-stress-stimulated glucose transport in skeletal muscle."Am J Physiol Endocrinol Metab. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] C.Son, K.Hosoda, et al.: "Reduction of diet-induced obesity in mice overexpressing uncoupling protein 3 in skeletal muscle."Diabetologia. 47. 47-54 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Takahashi-Yasuno, K.Hosoda, et al.: "Leptin receptor polymorphism is associated with serum lipid levels and impairment of cholesterol lowering effect by simvastatin in Japanese men."Diabetes Res Clin Pract. 62(3). 169-175 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] J.Fujikura, E.K.Hosoda, et al.: "Differentiation of embryonic stem cells is induced by GATA factors."Genes and Development. 16. 784-789 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Iwakura, K.Hosoda, et al.: "Ghrelin expression in islet cell tumors- augmented expression of ghrelin in a case of glucagonoma with multiple endocrine neoplasm typeI."J.Clin.Endocrinol Metab. 87. 4885-4888 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ariyasu, H.Hosoda, et al.: "Delayed short-term secretory regulation of ghrelin in obese animals : evidence by a specific RIA for the active form of ghrelin."Endocrinology. 143. 3341-3350 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Miyawaki, K.Hosoda, et al.: "Clinical implications of leptin and its potential humoral regulators in long-term low-calorie diet therapy for obese humans."Eur.J.Clin.Nutr. 56. 593-600 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Toyoda, K.Hosoda, et al.: "Possible involvement of the {alpha} 1 isoform of 5'AMP-activated protein kinase in oxidative-stress-stimulated glucose transport in skeletal muscle."Am J Physiol Endocrinol Metab. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] C.Son, K.Hosoda, et al.: "Reduction of diet-induced obesity in mice overexpressing uncoupling protein 3 in skeletal muscle."Diabetologia. 47. 47-54 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Takahashi-Yasuno, K.Hosoda, et al.: "Leptin receptor polymorphism is associated with serum lipid levels and impairment of cholesterol lowering effect by simvastatin in Japanese men."Diabetes Res Clin Pract. 63(3). 195-175

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] J.Fujikura, E.K.Hosoda, et al.: "Differentiation of embryonic stem cells is induced by GATA factors."Genes and Development. 16. 784-789 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Iwakura, K.Hosoda, et al.: "Ghrelin expression in islet cell tumors-augmented expression of ghrelin in a case of glucagonoma with multiple endocrine neoplasm typeI."J.Clin.Endocrinot Metab. 87. 4885-4888 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ariyasu, H.Hosoda, et al.: "Delayed short-term secretory regulation of ghrelin in obese animals: evidence by a specific RIA for the active form of ghrelin."Endocrinology. 143. 3341-3350 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Miyawaki, K.Hosoda, et al.: "Clinical implications of leptin and its potential humoral regulators in long-term low-calorie diet therapy for obese humans."Ear.J.Clin.Nutr. 56. 593-600 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Toyoda, K.Hosoda, et al.: "Possible involvement of the {alpha} 1 isoform of 5'AMP-activated protein kinase in oxidative-stress-stimulated glucose transport in skeletal muscle"Am J Physiol Endocrinol Metab.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] C.Son, K.Hosoda, et al.: "Reduction of diet-induced obesity in mice overexpressing uncoupling protein 3 in skeletal muscle"Diabetologia. 47. 47-54 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Takahashi-Yasuno, K.Hosoda, et al.: "Leptin receptor polymorphism is associated with serum lipid levels and impairment of cholesterol lowering effect by simvastatin in Japanese men"Diabetes Res Clin Pract. 62(3). 169-175 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] J.Fujikura, K.Hosoda et al.: "Differentiation of embryonic stem cells is induced by GATA factors"Genes and Development. 16・7. 784-789 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Iwakura, K.Hosoda et al.: "Ghrelin expression in islet cell tumors-augmented expression of ghrelin in a case of glucagonoma with multiple endocrine neoplasm typeI"J. Clin. Endocrinol. Metab. 87・11. 4885-4888 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Ariyasu, K.Hosoda et al.: "Delayed short-term secretory regulation of ghrelin in obese animals : evidence by a specific RIA for the active form of ghrelin"Endocrinology. 143・9. 3341-3350 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Miyawaki, K.Hosoda et al.: "Clinical implications of leptin and its potential humoral regulators in long-term low-calorie diet therapy for obese humans"Eur. J. Clin. Nutr. 56・7. 593-600 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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