| Project/Area Number |
14571152
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
UEDA Yuji Kyoto Prefectural University of Medicine, Department of Surgery, Division of Digestive Surgery, Assistant Professor, 医学研究科, 助手 (60254356)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRASAKA Tetsuhiko Taiho Pharmaceutical Co., Ltd., Institute for Pathogenic Biochemistry in Medicine, Director, 病態医化学研究所, 所長
TERAMOTO Kazuo Toray Industries, Inc., Medical Device Res. Lab., Research Associate, "Speciality Polymer Design" Deputy Leader, 機能材料研究所, 主席研究員
YAMAGISHI Hisakazu Kyoto Prefectural University of Medicine, Department of Surgery, Division of Digestive Surgery, Professor, 医学研究科, 教授 (40128723)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Cancer Immunotherapy / Direct Hemoperfusion / Immunosuppressive Factor / Immunosuppressive Cytokine / TGFβ / VEGF / IL-6 |
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| Research Abstract |
We have done a fundamental research on the development of a new technology of direct hemoperfusion aiming to remove immunosuppressive cytokines from the blood of cancer-bearing hosts. Immunosuppressive substance adsorption (ISA) fiber was prepared by chemical modification of a polystyrene-based composite fiber. In brief, the composite fiber is treated with N-methylol-α-chloroacetamide in a mixture of sulfuric acid and nitrobenzene in the presence of paraformaldehyde. Sterile cylindrical plastic columns with a capacity of 2 ml were prepared by filling 0.4 g of ISA fiber. Tumor-bearing rats were made by implanting KDH-8 cells (hepatocelllar carcinoma) subcutaneously into the back of WKAH/Hm rats. Direct hemoperfusion of tumor-bearing rats using these columns significantly reduced the tumor growth and. prolonged the survival of them. These columns efficiently removed TGFb, VEGF, and IL-6 from the blood of tumor-bearing rats.
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