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Development of CD-DST sensitivity test and analysis of relationship between p53 related genes and prognosis.

Research Project

Project/Area Number 14571168
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionThe Tazuke Kofukai

Principal Investigator

ISHIDA Hisao  Kitano Hospital The Tazuke Kofukai Medical Research Institute., Fifth Department of Oncology Department of Thoracic Surgery, 医学研究所・第5研究部, 研究員 (40322771)

Co-Investigator(Kenkyū-buntansha) HATTORI Noboru  Kitano Hospital The Tazuke Kofukai Medical Research Institute., Fifth Department of Oncology Department of Thoracic Surgery, Chief-Researcher, 医学研究所・第5研究部, 主任研究員 (00283169)
MIYAKE Masayuki  Kitano Hospital The Tazuke Kofukai Medical Research Institute., Fifth Department of Oncology Department of Thoracic Surgery, Director, 医学研究所・第5研究部, 部長 (90250076)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsanti cancer drug / CD-DST / matrigel / p53
Research Abstract

Initially, we tried to establish many kinds of cell lines of lung cancer. The previous experiments showed that mixing 50% matrigel in this collagen was the most efficient for establishment of the cell lines. Furthermore, adding 10% of patients' serum in the above mixture made it easier to establish the cell line. Using this method, three kinds of AAH were established, which was the first success of establishment AAH cell lines in the collagen gel, and then we succeeded in almost 10 times cultures. At this stage, it was possible to analyze the mutation of p53. But, all three cases had wild type of p53 gene, and abnormality of p53 related genes for example, p21was not found. We also established cell lines on the lung cancers from the point of view of pathology. It was succeeded to establish 10 cell lines in Stage I lung cancer and 9 cell lines in Stage II. We tried to establishl3 cell lines in Stage III lung cancer that did chemotherapy before the operation, in contrast, only 4 cell lines were established because most of the parts were necrotic region. Then, 6 kinds of cell lines in StageIV were established from the specimens of metastasis region. From these points, we examined mutations of p53, not from extracted DNA from the resected specimens, but from those cell lines, which is important to avoid the contamination of normal cells. However, we could not find any relationships between mutations of p53 and stages. And also, no significant relationships between diseases free survival and p53 mutations were found. On the other hand, we could not find the significant relationship of anticancer drug sensitivity and the effect on the patients using the same drugs in the patients with the relapse of StageIII and StageIV lung cancer.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Ikeda, N. et al.: "Clinical Significance of Aminopeptidase N/CD13 Expression in Human Pancreatic Carcinoma."Clinical Cancer Research. 9. 1503-1508 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashida, H. et al.: "Clinical Significance of Transmembrane 4 Superfamily in Colon Cancer."British Journal of Cancer. 89. 158-167 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ding, Y. et al.: "Association of CC cChemokine Receptor 7 with Lymph Node Metastasis of Esophageal Squamous Cell Carcinoma."Clinical Cancer Research. 9. 3406-3412 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 三宅正幸: "肺癌の生物学的予後因子"呼吸器疾患を探る-腫瘍編-. 22-26 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 石田久雄 et al.: "癌の封じ込めによる肺切除適応の拡大 Enlargement of application for lung cancer surgery based on metastasis prevention."外科治療. 90. 85-86 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hattori, N. et al.: "The plasminogen activation system reduces fibrosis in the lung by a hepatocyte growth factor-dependent mechanism."American Journal of Pathology. 164. 1091-1098 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 三宅正幸 et al.: "これだけは知っておきたい-遺伝子医学の基礎知識"メデイカル ドゥ. 7 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimizu, K., Chin, K., Nakamura, T., Masuzaki, H., Ogawa, Y., Hosokawa, R., Niimi, A., Hattori, N., Sasayama, S., Nakao, K., Mishima, M., Nakamura, T., Ohi, M.: "Plasma leptin levels and cardiac sympathetic function in patients with obstructive sleep apnoea-hypopnoea syndrome."THORAX. 57. 429-434 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sisson, TH., Hanson, KE., Subbotina, N., Patwardhan, A., Hattori, N., Simon, RH.: "Inducible lung-specific urokinase expression reduces fibrosis and mortality after lung injury in mice."Am J Physiol Lung Cell Mol Physiol. 283. L1023-L1032 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashida, H., Takabayashi, A., Tokuhara, T., Taki, T., Kondo, K., Kohno, N., Yamaoka, Y., Miyake M.: "Integrin α3 Expression as a Prognostic Factor in Colon Cancer : Association with MRP-1/CD9 and KAI1/CD82."INT J CANCER. 97. 518-525 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohno, M., Hasegawa, H., Miyake, M., Yamamoto, T., Fujita, S.: "CD151 Enhances Cell Motility and Metastasis of Cancer Cells in the Presence of Focal Adhesion Kinase."INT J CANCER. 97. 336-343 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashida, H., Takabayashi, A., Kanai, M., Adachi, M., Kondo, K., Kohno, N., Yamaoka, Y., Miyake, M.: "Aminopeptidase N Is Involved in Cell Motility and Angiogenesis : Its Clinical Significance in Human Colon Cancer."GASTRORNTEROLOGY. 122. 376-386 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim, KK., Flaherty, KR., Long, Q., Hattori, N., Sisson, TH., Colby, TV., Travis, WD., Martinex, FJ., Murray, S., Simon, RH.: "A plasminogen activator inhibitor-1 promoter polymorphism and Idiopathic interstitial pneumonia."Mol Med. 9. 52-56 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikeda, N., Nakajima, Y, Tokuhara, T., Hattori, N., Sho, M., Kanehiro, H, Miyake, M.: "Clinical Significance of Aminopeptidase N/CD13 Expression in Human Pancreatic Carcinoma."CLIN CANCER RES. 9. 1503-1508 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chin, K., Nakamura, T., Takahashi, K., Sumi, K., Ogawa, Y, Masuzaki, H., Muro, S., Hattori, N., Matsumoto, H., Niimi, A., Chiba, T., Nakao, K., Mishima, M., Ohi, M., Nakamura, T.: "Effects of obstructive sleep apnea syndrome on serum aminotransferase Levels in obese patients."Am J Med. 114. 370-376 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashida, H., Takabayashi, A., Tokuhara., T., Hattori, N., Taki, T., Hasegawa, H., Satoh, S., Kobayashi, N., Yamaoka, Y., Miyake, M.: "Clinical Significance of Transmenbrane 4 Superfamily in Colon Cancer."BRIT J CANCER. 89. 158-167 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hattori, Y., Nerusu, KC., Bhagavathula, N., Brennan, M., Hattori, N., Murphy, HS., Su, LD., Wang, TS., Johnson, TM., Varani, J.: "Vascular expression of matrix metalloproteinase-13 (collagenase-3) In basal cell carcinoma."Exp Mol Pathol.. 74. 230-237 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chuang-Tsai, S., Sisson, TH., Hattori, N., Tsai, CG., Subbotina, NM., Hanson, KE., Simon, RH.: "Reduction in fibrotic tissue formation in mice genetically deficient in Plasminogen avtivator inhibitor-1."AM J PATHOL. 163. 445-452 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ding, Y, Shimada, Y., Maeda, M., Kawabe, A., Kaganoi, J., Komoto, I., Hashimoto, Y, Miyake, M., Hashida, H., Imamura, M: "Association of CC cChemokine Receptor 7 with Lymph Node Metastasis of Esophageal Squamous Cell Carcinoma."CLIN CANCER RES. 9. 3406-3412 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hattori, N., Mizuno, S., Yoshida, Y., Chin, K., Mishima, M., Sisson, TH., Simon, RH., Nakamura, T., Miyake M.: "The Plasminogen activation system reduces fibrosis in the lung by a Hepatocyte growth factor-dependent mechanism."AM J PATHOL. 164. 1091-1098 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hattori, N., Miyake, M.: "Gene therapy for pulmonary diseases."Frontiers in Immuno-gene Therapy. 209-225 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikeda, N. et al.: "Clinical Significance of Aminopeptidase N/CD13 Expression in Human Pancreatic Carcinoma."Clinical Cancer Research. 9. 1503-1508 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hashida H, et al.: "Clinical Significance of Transmenbrane 4 Superfamily in Colon Cancer."British Journal of Cancer. 89. 158-167 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ding, Y. et al.: "Association of CC cChemokine Receptor 7 with Lymph Node Metastasis of Esophageal Squamous Cell Carcinoma."Clinical Cancer Research. 9. 3406-3412 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 三宅正幸: "肺癌の生物学的予後因子"呼吸器疾患を探る-腫瘍編-. 22-26 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 石田久雄 et al.: "癌の封じ込めによる肺切除適応の拡大 Enlargement of application for lung cancer surgery based on metastasis prevention."外科治療. 90. 85-86 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hattori, N. et al.: "The plasminogen activation system reduces fibrosis in the lung by a hepatocyte growth factor-dependent mechanism."American Journal of Pathology. 164. 1091-1098 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 三宅正幸 et al.: "これだけは知っておきたい-遺伝子医学の基礎知識"メディカル ドゥ. 7 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hashida, H. et al.: "Integrin α3 Expression as a Prognostic Factor in Colon Cancer : Association with MRP-1/CD9 and KAI1/CD82"International Journal of Cancer. 97. 518-525 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kohno, M. et al.: "CD151 Enhances Cell Motility and Metastasis of Cancer Cells in the Presence of Focal Adhesion Kinase"International Journal of Cancer. 97. 336-343 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hashida, H. et al.: "Aminopeptidase N Is Involved in Cell Motility and Angiogenesis : Its Clinical Significance in Human Colon Cancer"Gastroenterology. 112. 376-386 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ikeda, N. et al.: "Clinical Significance of Aminopeptidase N/CD13 Expression in Human Pancreatic Carcinoma"Clinical Cancer Research. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] 服部 登: "線溶系-呼吸器疾患とのかかわり"分子呼吸器病. 6. 58-60 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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