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Study for bile duct dilatation of the choledochal cysts inferred from nerve plexus.

Research Project

Project/Area Number 14571191
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionNagoya University

Principal Investigator

ANDO Hisami  Nagoya University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (60184321)

Co-Investigator(Kenkyū-buntansha) KANEKO Kenichiro  Nagoya University, University Hospital, Assistant Professor, 医学部附属病院, 講師 (90335042)
小倉 行雄  名古屋大学, 医学部附属病院, 医員
落合 恵子  名古屋大学, 医学部附属病院, 医員
勝野 伸介  名古屋大学, 医学部付属病院, 医員
原田 徹  名古屋大学, 医学部付属病院, 講師 (90345893)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordscholedochal cyst / bile duct dilatation / Hirschsprung's disease / Whole-mount preparation / hypoganglionosis / hypoganglionosis / 膵胆管合流異常 / 総胆管 / 神経叢 / 胆管
Research Abstract

1.This study analyzed the bile duct walls of patients with choledochal cyst as to whether the nerve fibers and nerve cells in the wall are abnormal or not. Bile ducts obtained from eight patients with choledochal cyst and three normal bile ducts without dilatation were studied. Whole-mount preparations displaying the myenteric plexus running beneath the outer surface of the bile duct were made by removing the mucosa, submucosa, fibromuscular layers under a stereomicroscope. Whole-mount preparations were immersed in 0.3% Triton X-phosphate buffer solution for 2 to 3 days. They were immunohistochemically stained using the streptavidin-biotin-peroxidase method after 6-to 7-day incubation with anti-protein gene product (PGP) 9.5 antibody as the primary antibody.
2.Developed networks of the myenteric plexus were observed in the fibromuscular layer near the outer surface. Distribution of the ganglia was clearly observed. Nerve strands of the patients were not significantly different from those of controls, but the number of ganglia of the patients decreased to one tenth of that of controls.
3.This distribution of nerve strands and ganglia in the bile duct wall of choledochal cyst is similar to that in the intestinal wall of hypoganglionosis, which implies the mechanism of dilatation of the bile duct in patients with choledochal cyst is the same as that of megacoion in patients with hypoganglionosis.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (24 results)

All 2005 2004 2002 Other

All Journal Article (14 results) Book (3 results) Publications (7 results)

  • [Journal Article] Bile infection contributes to intrahepatic calculi formation after excision of choledochal cysts.2005

    • Author(s)
      Kenitiro Kaneko
    • Journal Title

      Pediatr Surg Int 21・1

      Pages: 8-11

    • NAID

      10019041871

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Bile infection contributes to intrahepatic calculi formation after excision of choledochal cysts.2005

    • Author(s)
      Kenichiro Kaneko, et al.
    • Journal Title

      Pediatr Surg Int 21(1)

      Pages: 8-11

    • NAID

      10019041871

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 小児膵胆管合流異常における膵石(protein plug)2004

    • Author(s)
      金子健一朗
    • Journal Title

      小児外科 36・4

      Pages: 515-519

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 膵胆管合流異常小児例の診断上の問題点-成人例との対比-2004

    • Author(s)
      金子健一朗
    • Journal Title

      胆と膵 25・1

      Pages: 15-19

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] The role of midkine and pleiotropine in liver regeneration.2004

    • Author(s)
      Ochiai Keiko
    • Journal Title

      Liver Int 24・5

      Pages: 484-491

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Isolation and culture of biliary epithelial cells from bile duct remnants of patients with biliary atresia.2004

    • Author(s)
      Ochiai Keiko
    • Journal Title

      Pediatr Surg Int 20・9

      Pages: 985-688

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Activated pancreatic enzyme and pancreatic stone protein (PSP/reg) in bile of patients with pancreaticobiliary maljunction/choledochal cysts2004

    • Author(s)
      Ochiai Keiko
    • Journal Title

      Dig Dis Sci 49・12

      Pages: 1953-1956

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Problems in diagnosis of pancreatico-biliary maljunction in pediatric patients compaired with adult patients.2004

    • Author(s)
      Kenichiro Kaneko, et al.
    • Journal Title

      Tan to Sui 25(1)

      Pages: 15-19

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The role of midkine and pleiotropine in liver regeneration.2004

    • Author(s)
      Keiko Ochiai et al.
    • Journal Title

      Liver Int 24(5)

      Pages: 484-491

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isolation and culture of biliary epithelial cells from bile duct remnants of patients with biliary atresia.2004

    • Author(s)
      Keiko Ochiai et al.
    • Journal Title

      Pediatr Surg Int 20(9)

      Pages: 985-988

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Activated pancreatic enzyme and pancreatic stone protein (PSP/reg) in bile of patients with pancreaticobiliary maljunction/choledochal cysts.2004

    • Author(s)
      Keiko Ochiai et al.
    • Journal Title

      Dig Dis Sci 49(12)

      Pages: 1953-1956

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isolation and culture of biliary epithelial cells from bile duct remnants of patients with biliary atresia.2004

    • Author(s)
      Ochiai Keiko
    • Journal Title

      Liver Int 20・9

      Pages: 985-988

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Activated pancreatic enzyme and pancreatic stone protein (PSP/reg) in bile of patients with pancreaticobiliary maljunction/choledochal cysts.2004

    • Author(s)
      Ochiai Keiko
    • Journal Title

      Dig Dis Sci 49・12

      Pages: 1953-1956

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Protein plugs complicating pancreaticobiliary maljunction.2002

    • Author(s)
      Kenichiro Kaneko, et al.
    • Journal Title

      Jpn J Pediatr Surg 36(4)

      Pages: 515-519

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 看護のための最新医学講座〔第2版〕第4巻消化管疾患 ヒルシュスプルング病と類縁疾患(千葉勉 編集)2005

    • Author(s)
      安藤久實
    • Total Pages
      494
    • Publisher
      中山書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Hirshusprung disease and its related disorders.(Symphonia medica nursing. 2nd ed.(Ed Tutomu Chiba))2005

    • Author(s)
      Hisami Ando
    • Publisher
      Nakayama-Shoten, Tokyo JAPAN
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 看護のための最新医学講座〔第2版〕第4巻消化管疾患 ヒルシュスプルング病と類縁疾患(千葉 勉 編集)2005

    • Author(s)
      安藤久實
    • Total Pages
      494
    • Publisher
      中山書店
    • Related Report
      2004 Annual Research Report
  • [Publications] 篠原 剛 他: "術中胆管損傷"小児外科. 35・4. 443-446 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 原 神也 他: "自己免疫性肝炎と原発性硬化性胆管炎のoverlap症候群の1例"小児科臨床. 56・9. 1849-1854 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 篠原 剛 他: "胆管切除を必要としたchronic expanding hematomaの1例"胆道. 17・4. 429-433 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 金子 健一朗 他: "先天性胆道拡張症と肝内結石"胆と膵. 24・11. 759-762 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 安藤久貫: "小児肝胆道疾患の画像診断"小児外科. 34・3. 289-300 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 篠原剛: "膵胆管合流異常-最近の話題、診断(1)小児"臨床消化器内科. 17・10. 1435-1443 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 安藤久實: "胆道外科の要点と盲点-先天性胆道拡張症の診断と手術のKnack&Pitfalls"分光堂 幕内政敏、二村雄次. 379 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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