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Adenovirus-mediated Smad4 gene transfer inhibit.s tumor growth and metastasis of human pancreatic carcinoma

Research Project

Project/Area Number 14571211
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKyushu University

Principal Investigator

NAGAI Eishi  Kyushu University, Graduate School of Medical Sciences, Surgery and Oncology accistant, 大学病院, 助手 (30264021)

Co-Investigator(Kenkyū-buntansha) MIZUMOTO Kazuhiro  Kyushu University, Graduate School of Medical Sciences, Surgery and Oncology, 大学病院, 講師 (90253418)
TANAKA Masao  Kyushu University, Graduate School of Medical Sciences, Surgery and Oncology, Prof., 大学院・医学研究院, 教授 (30163570)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsPancreatic cancer / Smad 4 / Gene therapy / Inhibition of angiogenesis
Research Abstract

Pancreatic cancer is one, of the most difficult neoplasms to treat curatively, because of its highly invasive and metastatic potential. We previously generated an antagonist. for HOF which played an important role in invasion and metastasis of human pancreatic cancer. This HGF-antagonist., NK4 has competitive inhibitory effects on interaction between HGF and C-MET receptor. Furthermore, NK4 inhibits proliferation and migration of distinct. type of endotbelial cells stimulated by angiogenic factor. In our privios study, adnovirus-mediated NK4 gene therapy suppressed the growth, invasiveness and metastasis of human pancreatic cancer cell lines with high expression of c-MET. However, there are a few pancreatic cancer cell lines with low level of c-MET expression, so we focused on Smad 4/DPC4. SMAD4 is a critical cofactor in signal transduction pathways activated in response to transforming growth factor-beta (TGF-beta)-related ligands, regulating cell growth and differentiation. Smad4 has been reported to express the inhibitory effects of on pancreatic tumour invasion and angiogenesis. Adenovirus mediated gene transfer in a panel of SMAD4 homozygouscleleted human pancreatic tumor cell lines should restore SMAD4 protein ex-pression and function. Thus adenovirus mediated Smad 4 gene therapy may be another attractive option for the treatment of pancreatic cancer. We are now preparing recombinat aclenovirus for cell growth and invasiveness of pancreatic cancer cell lines. The tumor growth of adenovirus-NK4 infected pancreatic cancer cells in nude mice will be analyzed.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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