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The analysis of thymidine phospholyrase and mucin expression related to invasion and metastasis in pancreas cancer

Research Project

Project/Area Number 14571212
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKagoshima University

Principal Investigator

NATSUGOE Shoji  Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (70237577)

Co-Investigator(Kenkyū-buntansha) TAKAO Sonshin  Kagoshima University, Research Center for Life Science Resources, Professor, 生命科学資源開発研究センター, 教授 (80171411)
AKIYAMA Shinichi  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (60117413)
YONEZAWA Suguru  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (10175002)
AIKOU Takashi  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (60117471)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordspancreatic cancer / invasion and metastasis / thymidine phospholyrase / mucin / lymph node micrometastasis / VEGF-C / VEGF-D / リンパ節郭清 / 血管新生因子 / 浸潤-転移 / MUC1 / 微小転移 / 免疫組織化学
Research Abstract

We found the close relationship between overexpression of thymidine phosphorylase(TP) or membrane-bound type mucin(MUC1) and hematogeneous metastasis or poor prognosis in various type of cancer. The incidence of TP overexpression was high in pancreatic cancer. Cell movement and invasion was promoted by TP, but apoptosis was suppressed by TP. TP was closely related to invasion and metastasis in cancer cell because TP inhibitor suppressed the expression of TP. Furthermore, we found that TP was related to the HIF-1 pathway in avoidance of apoptosis. MUC1 expression correlated well with the malignant biological behavior in pancreatic cancer. We reported the new classification of intraductal papillary-mucinous tumor in the pancreas by intestinal type secretory mucin(MUC2) expression.
Pancreatic cancer is one of the most aggressive cancers in solid tumor. The high incidence of recurrent disease was found after curative resection. We examined the presence of lymph node micrometastasis in pancreatic cancer. Lymph node micrometastasis was highly found even in pN0 pancreatic cancer. The clinical significance should be clarified in the large number of patients, especially in the distant lymph node micrometastasis.
The expression of VEGF-C and -D was immunohistochemically examined in pancreatic cancer. The incidence of VEGF-C and -D overexpression was significantly higher in the marginal portion than in the central portion of the tumor. Prognosis of patients with high expression of both VEGF-C and -D in the marginal portion was significantly poorer than that of patients with loe expression of both VEGF-C and -D. The examination of VEGF-C and -D expression by biopsy specimens will be useful to predict the malignant property in pancreatic cancer.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (27 results)

All 2005 2004 2003 2002 Other

All Journal Article (17 results) Book (1 results) Publications (9 results)

  • [Journal Article] Impact of vascular endthelial growth factor-C and -D expression inhuman pancreatic cancer : its relationship to lymph node metastasis.2004

    • Author(s)
      Hiroshi Kurahara et al.
    • Journal Title

      Clinical Cancer Research 10

      Pages: 8413-8420

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Carcinoembryonic antigen messenger RNA expression using nested reverse transcription-PCR in the peripheral blood during follow-up period of patients who underwent curative surgery for biliary-pancreatic cancer : longitudinal analyses.2004

    • Author(s)
      Yuko Mataki et al.
    • Journal Title

      Clinical Cancer Research 10

      Pages: 3807-3814

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The antimetastatic role of thorombomodulin expression in islet cell-derived tumors and its diagnostic value2004

    • Author(s)
      Satoshi Iino et al.
    • Journal Title

      Clinical Cancer Research 10

      Pages: 6179-6188

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Impact of vascular endothelial growth factor-C and -D expression inhuman pancreatic cancer : its relationship to lymph node metastasis.2004

    • Author(s)
      Hiroshi Kurahara et al.
    • Journal Title

      Clinical Cancer Research 10(24)

      Pages: 8413-8420

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Carcinoembryonic antigen messenger RNA expression using nested reverse transcription-PCR in the peripheral blood during follow-up period of patients who underwent curative surgery for biliary-pancreatic cancer : longitudinal analyses.2004

    • Author(s)
      Yuko Mataki et al.
    • Journal Title

      Clinical Cancer Research 10(11)

      Pages: 3807-3814

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Carcinoembryonic antigen messenger RNA expression using nested reverse transcription PCR in the peripheral blood during follow-up period of patients who underwent curative surgery for biliary-pancreatic cancer : longitudinal analyses.2004

    • Author(s)
      Mataki Y, Takao S, et al.
    • Journal Title

      Clinical Cancer Research 10

      Pages: 3807-3814

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Impact of vascular endothelial growth factor-C and -D expression inhuman pancreatic cancer : its relationship to lymph node metastasis.2004

    • Author(s)
      Kurahara H, Takao S, et al.
    • Journal Title

      Clinical Cancer Research 10

      Pages: 8413-8420

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Rapid immuno histochemical detection of lymph node metastasis during operation for upper gastrointestinal carcinoma2003

    • Author(s)
      Matsumoto M, Natsugoe S, et al.
    • Journal Title

      British Journal of Surgery 90

      Pages: 563-566

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Clinical significance of circulating tumor cell in blood molecular detection and tumor markers in esophageal cancer2003

    • Author(s)
      Nakashima S, Natsugoe S, et al.
    • Journal Title

      Surgery 133

      Pages: 162-169

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 膵癌の治療戦略:分子生物学的アプローチによる血行性転移の予測2003

    • Author(s)
      高尾尊身, 又木雄弘, 他
    • Journal Title

      外科治療 88

      Pages: 79-85

    • NAID

      40019866806

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Intraoperative molecular detection of circulating tumor cells by reverse transcription-polymerase chain reaction in patients with biliary-pancreatic cancer is associated with hematogeneous metastasis2002

    • Author(s)
      Keiichirou Uchikura et al.
    • Journal Title

      Annals of Surgical Oncology 9

      Pages: 364-370

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] New classification of pancreatic intraductal papillary-mucinous tumor by mucin expression : its relationship with potential for malignancy2002

    • Author(s)
      Akiko Nakamura et al.
    • Journal Title

      Journal of Pathology 197

      Pages: 201-210

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tymidine phosphorylase suppresses Fas-induced apoptotic signal transduction independent of its enzymatic activity2002

    • Author(s)
      Shinichrou Mori et al.
    • Journal Title

      Biochemical and Biophysical Research Communications 295

      Pages: 300-305

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Intraoperative molecular detection of circulating tumor cells by reverse transcription-polymerase chain reaction in patients with biliary-pancreaticcancer is associated with hematogeneous metastasis2002

    • Author(s)
      Keiichirou Uchikura et al.
    • Journal Title

      Annals of Surgical Oncology 9(4)

      Pages: 364-370

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] New classification of pancreatic intraductal papillary-mucinous tumor by mucin expression : its relationship with potential for malignancy2002

    • Author(s)
      Akiko Nakamura et al.
    • Journal Title

      Journal of Pathology 197(2)

      Pages: 201-210

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Research Tymidine phosphorylase suppresses Fas-induced Apoptotic signal transduction independent of its enzymatic activity2002

    • Author(s)
      Shinichrou Mori et al.
    • Journal Title

      Biochemical and Biophysical Communication 295(2)

      Pages: 300-305

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The antimetastatic role of thorombomodulin expression in islet cell-derived tumors and its diagnostic value

    • Author(s)
      Satoshi Iino et al.
    • Journal Title

      Clinical Cancer Research 10(18)

      Pages: 6179-6188

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] The Diversity of Gastric Carcinoma2005

    • Author(s)
      Shoji Natsugoe
    • Total Pages
      353
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Matsumoto M, Natsugoe S, et al.: "Rapid immunohistochemical detection of lymph node micrometastasis during operation for upper gastrointestinal carcinoma."Br.J.Surg. 90. 563-566 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakashima S, Natsugoe S, et al.: "Clinical significance of circulating tumor cells in blood by molecular detection and tumor markers in esophageal cancer."Surgery. 133. 162-169 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 高尾尊身, 又木雄弘, 他: "膵癌の治療戦略:分子生物学的アプローチによる血行性転移の予測"外科治療. 88. 79-85 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Horinouchi M, Nagata K, et al.: "Expression of different glycoforms of membrane mucin (MUC1) and secretory mucin (MUC2, MUC5AC and MUC6) in pancreatic neoplasms."Acta Histochem Cytochem. 36. 443-453 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hiroyoshi Yoh: "A novel fluorometric invasion assay for quantitative determination of tumor cell invasion"Laboratory Investigation. 82. 109-111 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Keiichirou Uchikura: "Intro operative molecular detection of circulating tumor cells by reverse transcription-polymerase chain reaction in patients with biliary pancreatic cancer is associated"Annals of Surgical Oncology. 9. 364-370 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshito Masuyama: "Comparison of matrix metalloproteinase expression between primary tumors with or without liver metastasis in pancreatic and colorectal carcinomas"Journal of Surgical Oncology. 80. 105-110 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akiko Nakamura: "New classification of pancreatic introductal papillary-mucinous tumor by mucin expression : its relationship with potential for malignancy"Journal of Pathology. 197. 201-210 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shinichirou Mori: "Thymidine phosphorylase suppresses Fas-induced apoptotic signal transduction independent of its enzymatic activity"Biochem Biophys Res Commun. 295. 300-305 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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