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Development of pancreatic stem cell and β-cell for cell therapy

Research Project

Project/Area Number 14571236
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionTokyo Women's Medical University

Principal Investigator

TSUCHIYA Mariko  Tokyo Women's Med.Univ., Sch of Med., Associate Prof., 医学部, 講師 (00266826)

Co-Investigator(Kenkyū-buntansha) TSUCHIYA Ken  Tokyo Women's Med.Univ., Sch of Med., Associate Prof., 医学部, 講師 (00246472)
大河原 久子  東京女子医科大学, 医学部, 助教授 (10075468)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsInsulin / islet / transcription factor / maf A / maf B / c-maf / porcine / βcell / maf / 転写因子 / ラ氏島 / インスリン / pancreatis β cell / Insulin / PDX-1 / porcine / Glucose Response / 膵β細胞 / グルコース反応性 / ブタ
Research Abstract

Maf is a family of transcription factor proteins, and maf A is a strong transactivator of insulin in cell lines. The induction of large mafs expression in rat pancreas under acute ischemic conditions in our previous study suggests that mafs may be involved not only in the regulation of cell function, but also in the development and differentiation of the pancreas. The present study investigated the expression profiles of the large maf family in the porcine pancreas and in primary culture cells. In the newborn pancreas tissue, the formation of islets was not clear compared with that in the adult pancreas tissue. Maf A- and c-positive cells were more diffuse and intensely stained in the pancreas tissue scatted throughout with fewer maf B positive clustering cells around at centroacinar area. In contrast, islet formation was more apparent and positive staining for maf A and c tended to be prominent in the islets of the adult pancreas tissue, As to endocrine function, maf A corresponds to … More insulin-and c-maf to glucagon-secreting cells, based on the results of a double staining study. Mafs mRNA levels measured by real-time PCR were overall expressed in the pancreas tissue. Mafs mRNA expression was also detectable in the primary cultures of endocrine cells freshly isolated from the newborn pancreas tissue. Each maf may be regulatory expressed and specific and independent transactivator of endocrine function. Maf B may also have other potentials for structure formation or hormone secretion. Fetal and adult human pancreas tissues stained positive for maf similarly as porcine, suggesting that porcine pancreas can be one prototype of human pancreas. Preliminarily, pancreas was small size but formed in newborn of c-maf knockout mice. In conclusion, large mafs were identified in pancreas and cultured endocrine cells, and the expression level and localization differ in newborn and adlut pancreas tissue. Mafs may play roles in establishing endocrine function during pancreatic endocrine cell differentiation in independent and specific manner. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (10 results)

All 2004 2003 2002 Other

All Journal Article (8 results) Publications (2 results)

  • [Journal Article] Developmental contribution of c-maf in the kidney2004

    • Author(s)
      Imaki J.
    • Journal Title

      Biochem Biophys Res Commun 320

      Pages: 1323-1327

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of GLP-1 or porcine pancreatic endocrine cell proliferation and insulin secretion2004

    • Author(s)
      Nagai K
    • Journal Title

      Pancreas 28

      Pages: 138-145

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development contribution of c-maf in the kidney2004

    • Author(s)
      Imaki J
    • Journal Title

      Biochem Biophys Res Commun 320

      Pages: 1323-1327

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of GLP-1 on porcine pancreatic endocrine cell proliferation and Insulin secretion2004

    • Author(s)
      Nagai K
    • Journal Title

      Pancreas 28

      Pages: 138-145

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of GLP-1(Glucogon-like Peptide1:7-36 Amide) on porcine pancreatic cell proliferation and insulin secretion2004

    • Author(s)
      Nagai K, Tsuchiya K, Ezaki T, Tsuchiya M, Ohgawara H
    • Journal Title

      Pancreas 28

      Pages: 138-145

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Analysis of gene expression and insulin secretion by monolayer-forming adult porcine pancreatic endocrine cells2003

    • Author(s)
      Tsuchiya M
    • Journal Title

      Pancreas 26

      Pages: 71-75

    • NAID

      10016163415

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysis of gene expression and insulin secretion by monolayer-forming adult porcine pancreatic endocrine cell2003

    • Author(s)
      Tsuchiya M
    • Journal Title

      Pancreas 26

      Pages: 71-75

    • NAID

      10016163415

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 再生医療の最前線 膵再生2002

    • Author(s)
      土谷まり子
    • Journal Title

      小児内科 34

      Pages: 94-92

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kazutaka Nagai: "Effect of GLP4 On Porcine Pancreatic Cell Proliferation and Insulin Secretion"Pancreas. 28(2). 138-147 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mariko Tsuchiya, K.Tsuchiya, Y.Iwami, H.Ohgawara: "Analysis of Gene expression and Insulin Secretion by Monolayer-Forming Adult Porcine Pancreatic Endocrine Cells"Pancreas. 26(1). 71-75 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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