| Project/Area Number |
14571237
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Toho University |
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Principal Investigator |
SUMIYAMA Y Toho University, Faculty of Medicine, Professor, 医学部, 教授 (10057648)
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| Co-Investigator(Kenkyū-buntansha) |
KUSACHI S Toho University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (70169978)
ARIMA Y Toho University, Faculty of Medicine, Instructor, 医学部, 助手 (50277290)
YOSHIDA Y Toho University, Faculty of Medicine, Instructor, 医学部, 助手 (10318298)
TANAKA H Toho University, Faculty of Medicine, Instructor, 医学部, 助手 (80307735)
YOICHI Y Toho University, Faculty of Medicine, Instructor, 医学部, 助手 (10349904)
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| Project Period (FY) |
2002 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2004: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2003: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | MRSA / sepsis / mouse / anti TSST-1 antibody / IL-1 / IFN-γ / bacterialtranslocation / TSST-1 / E.coli / エンドトキシン / 内因性感染 / MRSA腸炎 / 多臓器不全 / 大腸癌 / E.coil / エンドトキシ / マウスモデル / エンテロトキシン |
|---|
| Research Abstract |
We made gut-derived sepsis with MRSA in mice. The bacterial suspension was orally administered between days 1 and 3. To aid the colonization of MRSA, the normal intestinal flora of the mice was disturbed by administering ampicillin (ABPC) by subcutaneous injection daily between days 1 and 3. After the administration of bacteria, mice were then given 250 mg/kg of cyclophosphamide (CY) by intraperitoneal injection on day 1, 4 and 7.
We studied the efficacy of antiTSST-1 monoclonal antibody against gut-derived sepsis with MRSA.
Although the significant difference was not recognized, antiTSST-1 monoclonal antibody administrated group tended to show highly survival rate.
We also studied about host defense. WT mice, IL-1 KO and IFN-γ KO mice were used in gut-derived sepsis with MRSA. Although WT mice were survived more than 80%, IL-1 KO mice died all. The numbers of viable bacteria in organs were significantly high level in the IL-1 KO mice.
IFN-γ KO mice were tend to die than WT mice.
IL-1 and IFN-γ were indicated that they were one of important host defense cytokines.
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