INHIBITORY EFFECT OF ANTISENSE OLIGONUCLEOTIDE FOR KERATINOCYTE GROWTH FACTOR FAMILY ON COLON CANCER CELLS
| Project/Area Number |
14571240
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | NIPPON MEDICAL SCHOOL |
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Principal Investigator |
WATANABE Masanori NIPPON MEDICAL SCHOOL, INSTITUTE OF GASTROENTEROLOGY, ASSISTANT PROFESSOR, 医学部, 講師 (70267227)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Colorectal cancer / Growth factor / Keratinocyte growth factor / Competitive RT-PCR / In situ hybridization / Antisense oligonucleotide / アンチセンスオリゴ / Keratinocyte Growth Factor (KGF) / short interfering RNA (siRNA) |
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| Research Abstract |
PURPOSE : This study was performed to clarify the growth inhibitory effect of antisense oligo-nucleotide of FGF-7 (Keratinocyte Growth Factor, KGF) or FGF-10 (KGF-2) on colon cancer cells.
MATERIALS and METHODS : The expression and localization of FGF-7, FGF-10 and their receptor (KGFR) in human colon cancer tissue and colon cancer cell lines were examined using RT-PCR, immunohistochemistry and in situ hybridization. Antisense oligonucleotides for FGF-7 or FGF-10 mRNA were transferred into colon cancer cell lines and the growth inhibitory effect was elucidated.
RESULTS and DISCOUSION : FGF-7 and FGF-10 and their mRNAs were localized in fibroblasts and colon cancer cells. KGFR mRNA was localized in colon cancer cells. These findings indicate that FGF-7 and FGF-10 may contribute to colon cancer cell growth via autocrine and paracrine mechanisms. FGF-10 antisense oligonucleotide inhibited the FOF-10 protein synthesis in colon cancer cells. Furthermore the antisense oligonucleotide inhibited the growth of colon cancer cells.
CONCLUSION : KGF family (FGF-7,FGF-1O and KGFR) may be one of the key factors for growth inhibitory therapy of colon cancer cells.
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Report
(3 results)
Research Products
(10 results)
