| Project/Area Number |
14571250
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | JAPANESE FOUNDATION FOR CANCER |
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Principal Investigator |
YAMAMOTO Junji JAPANESE FOUNDATION FOR CANCER, CANCER INSTITUTE, 癌研究所, 研究員 (40342654)
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| Co-Investigator(Kenkyū-buntansha) |
SAIURA Akio JAPANESE FOUNDATION FOR CANCER, CANCER INSTITUTE, 癌研究所, 研究員 (70345221)
阪本 良弘 財団法人癌研究会, 癌研究所, 研究員 (70343746)
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| Project Period (FY) |
2002 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | colorectal liver metastasis / PSEUDOCAPSULE / DNA microarray / pathology / 肝腫瘍 / 腫瘍-非腫瘍間線維組織 / 血管侵襲 / 転移性肝腫瘍 / 胃癌 / 大腸癌 / 切除後予後 |
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| Research Abstract |
PURPOSE : To clarify the cause and pathogenesis of pseudocapsule around the liver tumor, liver metastatic tumor from colorectal cancer were analyzed pathomorphologically and by DNA microarray. MATERIAL AND METHODS : 1.Harvesting of tissue RNA and DNA microarray analysis From the frozen specimen of resected liver tumor from colorectal cancer, total RNA was collected by Laser microdissection from cancer tissue near the pseudocapsule and noncancerous liver parenchyma. Five patients whose tumor had thick capsule and the other five whose tumor showed no capsule were selected for analysis, respectively. For analysis, GeneSpring and d-Chip software was used. RESULTS : From the analysis using GeneSpring software the genes possibly related to pseudocapsule formation were more frequently found in the cancer tissue than in the normal tissue. The d-Chip picked out 47 genes which showed the different level of expression between cancer and noncancerous tissues.
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