| Project/Area Number |
14571254
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Akita University |
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Principal Investigator |
YAMAMOTO Hiroshi (2003) Akita University, School of Medicine, Associate Professor, 医学部, 助教授 (10270795)
加賀谷 聡 (2002) 秋田大学, 医学部, 助手 (60323143)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIBASHI Kazuyuki Akita University, School of Medicine, Lecturer, 医学部, 講師 (00291617)
YAMAMOTO Fumio Akita University, School of Medicine, Professor, 医学部, 教授 (00127474)
山本 浩史 秋田大学, 医学部, 助教授 (10270795)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Cell Transplantation / pacemaker cell / 細胞移植 |
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| Research Abstract |
It has already been reported about therapeutic methodes for heart failure using a tissue engineering technique that skeletal myoblasts, embryonic cardiomyocytes, or marrow stem cells is directly transplanted to the myocardium. It has not been investigated whether or not myocardial conduction failure is cured by injecting the cells of the myocardial conduction system. Antiarrhythmic surgical approaches to chronic arrhythmia such as Maze operation and dual chamber pacing have been extensively challenged, but having limitations of curative effectiveness. We have, therefore, focused on the effect of pacemaker cell transplantation.
Two protocols on allo-transplantation of pacemaker cells were performed, the first one includes (1) transplantation of primarily cultured atrial or ventricular myocytes to the atrial myocardium and (2) transplantation of the beating cells retrieved from the cultured marrow interstitial cells to the atrial myocardium. During the given research period for this scien
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tific research grant, we were not able to retrieve beating cells from atrial myocytes or marrow interstitial cells, whereas, we had a successful result about the primary culture (for few days) of adult canine ventricular myocytes. We have planned to transplant the primarily cultured cells from ventricular myocytes to the canine myocardium with the sinus nodes destroyed.
The second one is the effect of allo-transplantation of the myocardial tissue including the sinus nodes, which results in (1) the allo-transplantation restoring the induced bradycardia in the canine with the sinus nodal tissue excised, (2) obtaining successful myocardial pacing by electrically pacing the transplanted tissue (including the sinus nodes) and (3) altered P-wave configuration and bradycardia during the aculte phase of the allo-transplantation. Further investigation will be planned in terms of elucidating (1) the nature of transmition of myocardial excitation in sinus node transplantation and (2) the effect of allo-transplantation of cryopreserved sinus node tissue. Less
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