| Project/Area Number |
14571279
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Keio University |
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Principal Investigator |
WATANABE Masazumi Keio University, School of Medicine, Assistant, 医学部, 助手 (90201227)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Koichi Keio University, School of Medicine, Professor, 医学部, 教授 (80051704)
HASEGAWA Naoki Keio University, School of Medicine, Assistant Professor, 医学部, 講師 (20198724)
後藤 太一郎 慶應義塾大学, 医学部, 助手 (80317148)
神山 育男 慶應義塾大学, 医学部, 助手 (70317147)
木村 吉成 慶應義塾大学, 医学部, 助手 (80327535)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Bronchoscopy / Microsampling method / Lung Cancer / Tumor marker / CEA / CYFRA |
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| Research Abstract |
Background: Making a preoperative pathologic diagnosis in patients with small lung nodules remains challenging. We have developed a new, noninvasive bronchoscopic microsampling probe to examine biochemical substances in epithelial lining fluid. We used this probe to measure tumor markers in fluid from tissues surrounding lung nodules <30 mm in diameter to test its adjunctive diagnostic utility in lung cancer.
Methods: In 16 patients, epithelial lining fluid was collected in triplicate or duplicate from tissue within 2 cm of small peripheral lung nodules and from the contra-lateral lung. The diagnosis of adenocarcinoma was surgically confirmed in all patients. Fifteen patients without lung cancer were controls. Concentrations of carcinoembryonic antigen, cytokeratin fragment 19, and sialy1 SSEA-1 were measured in the fluid.
Results: Carcinoembryonic antigen and cytokeratin fragment 19 concentrations were significantly higher in fluid near the nodules (mean = 20.5 and 237.8 ng/mg, respectively) than on the contralateral sides (1.9 and 82.6, respectively) or in fluid collected from the controls (2.5 and 5.1, respectively).
Conclusions: Measurements of carcinoembryonic antigen and cytokeratin fragment 19 collected by our microsampling probe may be a useful diagnostic adjunct in patients with small peripheral lung nodules.
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