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Tissue Engineering Heart Valve Leaflets.

Research Project

Project/Area Number 14571284
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionTOKYO WOMEN'S MEDICAL UNIVERSITY

Principal Investigator

MATSUMURA Goki  TOKYO WOMEN'S MEDICAL UNIVERSITY, 医学部, 助手 (20297469)

Co-Investigator(Kenkyū-buntansha) SHIN'OKA Toshiharu  TOKYO WOMEN'S MEDICAL UNIVERSITY, 医学部, 助教授 (20192122)
AOKI Mitsuru  TOKYO WOMEN'S MEDICAL UNIVERSITY, 医学部, 講師 (80175736)
KUROSAWA Hiromi  TOKYO WOMEN'S MEDICAL UNIVERSITY, 医学部, 教授 (50075511)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsTISSUE ENGINEERING / HYBRID VESSELS / AUTOLOGOUS CELLS / BONE MARROW CELLS / ENDOTHELIAL CELLS / BIOABSORBABLE SCAFFOLD / 細胞培養 / 平滑筋細胞 / 内皮細胞 / 自己細胞 / 心臓弁
Research Abstract

Various vascular grafts are commonly used in the reconstruction of cardiovascular tissues. However, currently used prosthetic or bioprosthetic materials lack growth potential and, therefore, subsequently require replacement in pediatric patients as they mature. Tissue engineering (TE) is a new discipline that offers the potential to create replacement structures from autologous cells and biodegradable polymer scaffolds. Because TE constructs contain living cells, they may have the potential to grow, self-repair, and self-remodel. Tissue Engineered Autografts (TEAs) were made by seeding 4-6 x 10^6 of mixed cells obtained from femoral veins of mongrel dogs onto valved patch-shape biodegradable polymer scaffolds composed of a polyglycolic acid (PGA) non-woven fabric sheet and a co-polymer of l-lactide and- caprolactone (N=4). After 7 days, the pulmonary valve leaflet of the same dogs was replaced with TEAs. After 3, 4, 5 and 6 months, angiographies were performed, and the dogs were sacrif … More iced. The implanted TAs were examined both grossly and immunohistologically. The implanted TEAs showed no evidence of stenosis or dilatation. No thrombus was found inside the TEAs, even without any anticoagulation therapy. Remnants of the polymer scaffolds were not observed in all specimens, and the overall gross appearance appeared similar to that of native pulmonary valves. Immunohistological staining revealed the presence of Factor VIII positive nucleated cells at the luminal surface of the TEAs. In addition, lesions were observed where α-smooth muscle actin and desmin positive cells existed. Implanted TEAs contained a sufficient amount of extracellular matrix, and showed neither occlusion nor aneurysmal formation. In addition, endothelial cells were found to line the luminal surface of each TEA. These results strongly suggest that 'ideal' grafts with anti-thrombogenicity can be produced. Because both our laboratory and clinical experiences are quite encouraging, we suggest that the TE approach may play an important role as an alternative method to transplantation and to the use of artificial organs in the field of pediatric cardiovascular surgery. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (23 results)

All 2003 2002 2001 Other

All Journal Article (13 results) Publications (10 results)

  • [Journal Article] Long-Term Histologic Findings in Pulmonary Arteries Reconstructed with Autologous Pericardium2003

    • Author(s)
      Hibino N, Shin'oka T, Kurosawa H
    • Journal Title

      The New England Journal of Medicine 348

      Pages: 865-867

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Successful application of tissue engineered vascular autografts : clinical experience2003

    • Author(s)
      Matsumura G, Hibino N, Ikada Y, Kurosawa H, Shin'oka T
    • Journal Title

      Biomaterials 24

      Pages: 2303-2308

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] First Evidence That Bone Marrow Cells Contribute to the Construction of Tissue-Engineered Vascular Autografts In vivo2003

    • Author(s)
      Matsumura G, Sachiko Miyagawa-Tomita, Shin'oka T, Ikada Y, Kurosawa H
    • Journal Title

      Circulation 108

      Pages: 1729-1734

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Extracardiac total cavopulmonary connection using a tissue-engineered graft2003

    • Author(s)
      Isomatsu Y, Shin'oka T, Matsumura G, Hibino N, Konnuma T, Nagatsu M, Kurosawa H
    • Journal Title

      J Thorac Cardiovasc Surg 126

      Pages: 1958-1962

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Tissue Engineered Heart Valves : Autologous Cell Seeding on Biodegradable Polymer Scaffold2002

    • Author(s)
      T.Shinoka
    • Journal Title

      Artificial Organ 26

      Pages: 522-526

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] A Successful Clinical Application of Tissue Engineered Graft for Extracardiac Fontan Operation2002

    • Author(s)
      Naito Y, Imai Y, Shin'oka T, Aoki M, Kashiwagi, Konuma T, Kurosawa H.
    • Journal Title

      Journal of Thoracic & Cardiovascular Surgery 125

      Pages: 419-420

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Effects of dilutional and modified ultrafiltration in plasma endothelin-1 and pulmonary vascular resistance after the fontan procedure2002

    • Author(s)
      T.Hiramatsu, Y.Imai, H.Kurosawa, Y.Takanashi, M.Aoki, T.Shin'oka
    • Journal Title

      Ann Thorac Surg 73

      Pages: 862-865

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] An arterial switch operation for a concordant crisscross heart with the complete transposition of the great arteries2002

    • Author(s)
      Kashiwagi J, Imai Y, Aoki M, Shin'oka T, Hagino I, Nakazawa M
    • Journal Title

      Journal of Thoracic & Cardiovascular Surgery 124

      Pages: 176-178

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Midterm results of surgical treatment of systemic ventricular outflow obstruction in Fontan patients2002

    • Author(s)
      T.Hiramatsu, Y.Imai, H.Kurosawa, Y.Takanashi, M.Aoki, T.Shin'oka
    • Journal Title

      Ann Thorac Surg 73

      Pages: 1282-1285

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Tissue Engineered Heart Valves : Autologous Cell Seeding on Biodegradable Polymer Scaffold2002

    • Author(s)
      Shin'oka T
    • Journal Title

      Artificial Organ 26

      Pages: 522-526

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] A Successful Clinical Application of Tissue Engineered Graft for Extracardiac Fontan Operation2002

    • Author(s)
      Naito Y, Imai Y, Shin'oka T, Kashiwagi J, Aoki M, Watanabe M, Matsumura G, Kosaka Y, Konuma T, Hibino N, Murata A, Miyake T, Kurosawa H
    • Journal Title

      J Thorac Cardiovasc Surg 125

      Pages: 419-420

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] An arterial switch operation for a concordant crisscross heart with the complete transposition of the great arteries2002

    • Author(s)
      Kashiwagi J, Imai Y, Aoki M, Shin'oka T, Hagino I, Nakazawa M
    • Journal Title

      J Thorac Cardiovasc Surg 124

      Pages: 176-178

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Transplantation of a Tissue Engineered Pulmonary Artery2001

    • Author(s)
      Shinoka T, Imai Y, Ikada Y
    • Journal Title

      The New England Journal of Medicine 344

      Pages: 532-533

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 新岡俊治, 松村剛毅, 日比野成俊, 内藤祐次, 小坂由道, 黒澤博身: "骨髄細胞を使用する再生血管移植の臨床"日本内科学会誌. 92. 11776-11780 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 新岡俊治: "大口径血管の再生医学"ドクターズマガジン. 2003.2. 40-41 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小坂由道, 新岡俊治: "生体吸収性人工血管の臨床応用"田畑泰彦編ここまで進んだ再生医療の実際. 96-103 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hibino N, Shin'oka Kurosawa H: "Long-Term Histologic Findings in Pulmonary Arteries Reconstructed with Autologous Pericardium"The New England Journal of Medicine. 348. 865-867 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsumura G, Hibino N, Ikada Y, Kurosawa H, Shin'oka T: "Successful application of tissue engineered vascular autografts : clinica experience"Biomaterials. 24. 2303-2308 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Shinoka: "Tissue Engineered Heart Valves : Autologous Cell Seeding on Biodegradable Polymer Scaffold"Artificial Organ. 26. 522-526 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Naito Y, Imai Y, Shin'oka T, Aoki M, Kashiwagi, Konuma T, Kurosawa H: "A Successful Clinical Application of Tissue Engineered Graft for Extracardiac Fontan Operation"Journal of Thoracic & Cardiovascular Surgery. 125. 419-420 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Hiramatsu, Y.Imai, H.Kurosawa, Y.Takanashi, M.Aoki, T.Shin'oka: "Effects of dilutional and modified ultrafiltration in plasma endothelin-1 and pulmonary vascular resistance after the fontan procedure"Ann Thorac Surg. 73. 862-865 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kashiwagi J, Imai Y, Aoki M, Shin'oka T, Hagino I, Nakazawa M: "An arterial switch operation for a concordant crisscross heart with the complete transposition of the great arteries"Journal of Thoracic & Cardiovascular Surgery. 124. 176-178 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Hiramatsu, Y.Imai, H.Kurosawa, Y.Takanashi, M.Aoki, T Shin'oka: "Midterm results of surgical treatment of systemic ventricular outflow obstruction in Fontan patients"Ann Thorac Surg. 73. 1282-1285 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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