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Mechanisms responsible for alveolar fluid reabsorption during peri-operative period

Research Project

Project/Area Number 14571287
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionKanazawa Medical University

Principal Investigator

SAKUMA Tsutomu  Kanazawa Medical University, Thoracic Surgery, MD, PhD, Professor, 医学部, 教授 (90215674)

Co-Investigator(Kenkyū-buntansha) SAGAWA Motoyasu  Kanazawa Medical University, Thoracic Surgery, Associate Professor, MD, PhD, 医学部, 助教授 (70292274)
ISHIGAKI Masanobu  Kanazawa Medical University, Thoracic Surgery, Assistant Professor, MD, PhD, 医学部, 助手 (10247439)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsalveolar fluid clearance / perioperative / malnutrition / beta-adrenoceptor / prostaglandin / purinoceptor / CFTR / alveolar epithelium / 肺水腫 / 肺胞水分細胞 / 肺切除術 / カテコールアミン / 虚血再潅流肺傷害 / UTP / 交感神経刺激薬 / ナトリウムチャンネル / アミロライド / グリベンクラミド
Research Abstract

The objectives of this study were to determine whether malnutrition, selective β-adrenergic agonists, and purinoceptors had effects on alveolar fluid clearance in rat lungs, to determine whether catecholamine clearance from the alveolar spaces was correlated with the fluid transport capacity of the rat and human lungs, and to whether endogenous levels of catecholamine stimulated alveolar fluid clearance via cystic fibrosis transmembrane conductance regulator (CFTR) in human lungs. We found that malnutrition impaired amiloride-insensitive and dibutyryl cGMP-sensitive alveolar fluid clearance, but this effect was partially reversible by re-feeding, treatment with sodium glutamate, or β-adrenergic agonist therapy. Prostaglandin analogues do not change basal alveolar fluid clearance and the effect of terbutaline at normal dose on alveolar fluid clearance, but restored the stimulatory effect as a β_1-adrenergic antagonist in ex vivo rat lungs with an administration of high-dose β_2-adrenergic antagonist. A combined treatment of UTP and isoproterenol increased alveolar fluid clearance by 280% basal value. The effects of UTP in the presence and absence of isoproterenol were abolished by blockers of a P2 purinoceptor and chloride channels. Catecholamine clearance rate from the alveolar spaces is correlated with alveolar fluid clearance in the rat and human lungs. Endogenous catecholamine concentrations in edema fluid were not sufficient to stimulate the resolution of acute pulmonary edema. Exogenous epinephrine, but not norepinephrine, stimulated alveolar fluid clearance via CFTR in ex vivo human lung. These studies indicate that alveolar fluid clearance play an important role in resolution of pulmonary edema of patients who underwent pulmonary resection. Beta-adrenergic agonist therapy may be considered in a patient exposed to pulmonary edema after pulmonary resection.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (17 results)

All 2005 2004 Other

All Journal Article (15 results) Publications (2 results)

  • [Journal Article] Catecholamine clearance from alveolar spaces of rat and human lungs2005

    • Author(s)
      Sakuma T
    • Journal Title

      Respiration 72

      Pages: 189-196

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Catecholamine clearance from alveolar spaces of rat and human lungs.2005

    • Author(s)
      Sakuma T, Gu X, Sugita M, Sagawa M, Sakuda M, Toga H.
    • Journal Title

      Respiration 72(2)

      Pages: 189-196

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Catecholamine Clearance from Alveolar Spaces of Rat and Human Lungs2005

    • Author(s)
      Sakuma T, et al.
    • Journal Title

      Respiration (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Malnutrition impairs alveolar fluid clearance in rat lungs2004

    • Author(s)
      Sakuma T
    • Journal Title

      Am J Physiol Lung Cell Mol Physiol 286

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A prostacyclin analogue OP-41483α-CD restores the ability of a β_2-adrenergic agonist to stimulate alveolar fluid clearance in rats2004

    • Author(s)
      Sakuma T
    • Journal Title

      Surg Today 34

      Pages: 429-436

    • NAID

      10014173400

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Uridine 5'-triphosphate stimulates alveolar fluid clearance in the isolated rat lungs2004

    • Author(s)
      Sakuma T
    • Journal Title

      J Pharmacol Sci 72

      Pages: 420-425

    • NAID

      10014165787

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of α_1-,β_1-,β_2-,β_3-adrenergic agonists on alveolar fluid clearance in isolated rat lungs2004

    • Author(s)
      Gu X
    • Journal Title

      J Kanazawa Med Univ 29

      Pages: 91-96

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Malnutrition impairs alveolar fluid clearance in rat lungs.2004

    • Author(s)
      Sakuma T, Zhao Y, Sugita M, Sagawa M, Toga H, Ishibashi T, Nishio M, Matthay MA.
    • Journal Title

      Am J Physiol Lung Cell Mol Physiol 286

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A prostacyclin analogue OP-41483α-CD restores the ability of a β_2-adrenergic agonist to stimulate alveolar fluid clearance in rats.2004

    • Author(s)
      Sakuma T, Zhao Y, Sugita M, Sagawa M, Hida M, Toga H.
    • Journal Title

      Surg Today 34(5)

      Pages: 429-436

    • NAID

      10014173400

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Uridine 5' -triphosphate stimulates alveolar fluid clearance in the isolated rat lungs.2004

    • Author(s)
      Sakuma T, Gu X, Sugita M, Sagawa M, Sakuda M, Toga H
    • Journal Title

      J Pharmacol Sci 95

      Pages: 420-425

    • NAID

      10014165787

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of α_1-, β_1-, β_2-, β_3-adrenergic agonists on alveolar fluid clearance in isolated rat lungs.2004

    • Author(s)
      Gu X, Sugita M, Sagawa M, Sakuda M, Osanai K, Toga H, Sakuma T.
    • Journal Title

      J Kanazawa Med Univ. 29(2)

      Pages: 91-96

    • NAID

      110004617776

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Malnutrition impairs alveolar fluid clearance in rat lungs2004

    • Author(s)
      Sakuma T, et al.
    • Journal Title

      Am J physiol Lung Cell Mol Physiol 286

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A prostacyclin analogue OP-41483a-CD restores the ability of a β2-adrenergic agonist to stimulate alveolar fluid clearance in rats2004

    • Author(s)
      Sakuma T, et al.
    • Journal Title

      Surgery Today 34・5

      Pages: 429-436

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Uridine 5'-triphosphate stimulates alveolar fluid clearance in the isolated rat lungs2004

    • Author(s)
      Sakuma T, et al.
    • Journal Title

      J pharmacol Sci 95

      Pages: 420-425

    • NAID

      10014165787

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of α_1-,β_1-,β_2-,β_3-adrenergic agonists on alveolar fluid clearance in isolated rat lungs2004

    • Author(s)
      Gu X, Sugita M, Sagawa M, Sakuda M, Osanai K, Toga H, Sakuma T
    • Journal Title

      J Kanazawa Med Univ 29・2

      Pages: 91-96

    • NAID

      110004617776

    • Related Report
      2004 Annual Research Report
  • [Publications] Tsutomu Sakuma et al.: "Malnutrition impairs alveolar fluid clearance in rat lungs"Am J Physiol. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsutomu Sakuma et al.: "A prostacyclin analogue OP-41483a-CD restores the ability of a β2-adrenergic agonist to stimulate alveolar fluid clearance in rats"Surg Today. (In press). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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