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Experimental therapy for malignant brain tumors using viral vector and neuronal stem cell

Research Project

Project/Area Number 14571312
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

ONO Yasuhiro  OKAYAMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE AND DENTISTRY, LECTURER, 大学院・医歯学総合研究科, 講師 (40294409)

Co-Investigator(Kenkyū-buntansha) ONO Shigeki  OKAYAMA UNIVERSITY, HOSPITALS, ASSISTANT, 医学部・歯学部附属病院, 助手 (40335625)
TOKUNAGA Kouji  OKAYAMA UNIVERSITY, HOSPITALS, LECTURER, 医学部・歯学部附属病院, 講師 (40294467)
SUGIU Kenji  OKAYAMA UNIVERSITY, HOSPITALS, LECTURER, 医学部・歯学部附属病院, 講師 (40325105)
富田 享  岡山大学, 大学院・医歯学総合研究科, 講師 (90237115)
田宮 隆  岡山大学, 大学院・医歯学総合研究科, 助教授 (50252953)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsBRAIN TUMORS / GENE TNERAPY / NEURONAL STEMM CELL / VIRAL VECTOR
Research Abstract

We have investigated the feasibility of gene transduction for braintumors using adenovirus vector. Transduction of the cytosine deaminase gene into tumor cells followed by administration of 5-fluorocytosine was found to have strong antitumor effect against experimental malignant brain tumor in vitro and in vivo. We also found its toxicity and limited antitumor effect on infiltrating tumor cells in normal brain tissue which cause local tumor recurrence. The purpose of this research was to improve antitumor effect on infiltrating tumor cells within normal brain tissue while minimizing neurotoxicity on normal brain cells. We developed new method to attain high local concentration of toxic drug coverted by CD transduced cell. We employed neuronal stem cells and bone marrow stem cells which harbour therapeutic gene and track infiltrating tumor cells. We showed their therapeutic effect in vitro when cultured with glioma cells. Then we investigated tracking of stem cells to infiltrating tumor cell in rat brain tumor model. We are now investigating in vivo therapeutic effect using stern cells harboring therapeutic gene.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 黒住和彦 他: "Neurofibromatosis type1に合併した下垂体腺腫の1例"脳神経外科. 30. 741-745 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Terada et al.: "Prognostic value of loss of heterozygosity around three candidate tumor suppressor genes on chr.10q in astrocytomas"J Neuro-oncology. 58. 107-114 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kambara et al.: "Combined radiation and gene therapy for brain tumors with adenovirus-mediated transfer of CD and UPRT genes"Cancer Gene Therapy. 9. 840-845 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ono S et al.: "Time course of the diameter of the major cerebral arteries after subarachnoid hemorrhage using corrosion cast technique"Neurological Research. 25. 383-389 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sugiu K et al.: "Angioplasty and coiling of ruptured aneurysm with symptomatic vasospasm : technical case report"Surgical Neurology. 59. 413-417 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kurozumi K, Tabuchi A, Ono Y, Tamiya T, Ohmoto T, Furuta T, Hamasaki S.: "Pituitary adenoma associated with neurofibromatosis type 1 : case report"No Shinkei Geka. 30. 741-745 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Terada K, Tamiya T, Daido S, Kambara H, Tanaka H, Ono Y, Matsumoto K, Ito S, Ouchida M, Ohmoto T, Shimizu K.: "Prognostic value of loss of heterozygosity around three candidate tumor suppressor genes on chromosome 10q in astrocytomas."J Neurooncol. 58. 107-114 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kambara H, Tamiya T, Ono Y, Ohtsuka S, Terada K, Adachi Y, Ichikawa T, Hamada H, Ohmoto T.: "Combined radiation and gene therapy for brain tumors with adenovirus-mediated transfer of cytosine deaminase and uracil phosphoribosyltransferase genes."Cancer Gene Ther. 9. 840-845 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ono S, Date I, Onoda K, Ohmoto T.: "Time course of the diameter of the major cerebral arteries after subarachnoid hemorrhage using corrosion cast technique"Neurol Res. 25. 383-389 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sugiu K, Katsumata A, Ono Y, Tamiya T, Ohmoto T.: "Angioplasty and coiling of ruptured aneurysm with symptomatic vasospasm : technical case report."Surg Neurol. 59. 413-417 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ono S et al.: "Time course of the diameter of the major cerebral arteries after subarachnoid hemorrhage using corrosion cast technique"Neurological Research. 25・4. 383-389 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sugiu K et al.: "Angioplasty and coiling of ruptured aneurysm with symptomatic vasospasm : technical case report"Surgical Neurology. 59・5. 413-417 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 黒住和彦他: "Neurofibromatosis type 1に合併した下垂体腺種の1例"脳神経外科. 30. 741-745 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Terada, et al.: "Prognostic value of loss of hetorozygosity around three condidate timer suppressor genes on chr. 10z om astrpcutmas"J. Neuro Oncology. 58. 107-114 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kanbara, et al.: "Combined radiation and gene therapy for brain tumors with udenovirus-mediated transfer of CD and UPRT genes"Concer Gene Therapy. 9. 840-845 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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