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Possible role of astrocytes in the disease progression of experimental cerebral ischemia

Research Project

Project/Area Number 14571330
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionSaitama Medical University

Principal Investigator

ASANO Takao  Saitama Medical School, Medicine, Professor, 医学部, 教授 (70090496)

Co-Investigator(Kenkyū-buntansha) MORIKAWA Eiharu  Saitama Medical school, Medicine, Associate professor, 医学部, 助教授 (90251256)
MORI Takashi  Saitama Medical school, Medicine, Associate professor, 医学部, 助教授 (60239605)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsCerebral Ischemia / Permanent focal cerebral ischemia / Rat / S-100 / Astrocyte / 永久脳虚血モデル / S-100蛋白 / アストロサイト / 永久局所脳虚血モデル
Research Abstract

The present study was undertaken to examine in terms of the following three aspects ; (i) the temporal as well as topographical correlation between the astrocytic S100B synthesis and the occurrence of delayed infarct expansion ; (ii) the analysis on the possible mechanisms underlying the S100B-induced brain damage ; (iii) the analysis on the possible therapeutic effects of an agent that suppresses astrocytic S100B synthesis on ischemic brain damage.
1) In 2002, we focused on whether activated astrocytes accumulating within the peri-infarct area is related to the occurrence of delayed infarct expansion after ischemia. The results showed a positive temporal and topographical correlation between the enhanced synthesis of S-100B by activated astrocytes in the peri-infarct area and the occurrence of delayed infarct expansion.
2) In 2003, using an astrocytic modulating agent, (P)-(-)-2-propyloctanoic acid (suppression of astrocytic activation through the negative regulation of S-100 protein sy … More nthesis), we examined the underlying mechanism(s) of delayed infarct expansion after ischemia. The results clearly showed that the enhanced astrocytic activation and subsequent production of S-100B protein in the peri-infarct area contribute to the occurrence of delayed infarct expansion.
3) In 2004, to extend the possible clinical application of the agent against stroke, we examined the optimal dose and the therapeutic time window of the agent. The results showed the optimal dose of 10 mg/kg/day and a wide therapeutic time window ranging from 0 to 48 hours after ischemia.
In summary, the present study contributed to substantiate the idea that activated astrocytes in the peri-infarct area do act to aggravate the ischemic brain damage during the subacute phase of permanent focal cerebral ischemia. We also demonstrated that suppression of astrocytic activation by the agent (R)-(-)-2-propyloctanoic acid (arundic acid) leads to mitigation of delayed infarct expansion and early improvement of neurological deficits. Based on the above data obtained from the consecutive studies during the past three years (2002 to 2004), we conclude that pharmacological modulation of astrocytic activation may confer a novel therapeutic strategy against stroke. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (19 results)

All 2005 2004 2003 2002 Other

All Journal Article (9 results) Book (6 results) Publications (4 results)

  • [Journal Article] Arundic acid (ONO-2506) ameliorates ischemic brain damage by preventing astrocytic overproduction of S100B.2005

    • Author(s)
      Asano T, Mori T, et al.
    • Journal Title

      Current Drug Targets-CNS & Neurologic Disorder (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Arundic acid (ONO-2506) ameliorates ischemic brain damage by preventing astrocytic overproduction of S100B2005

    • Author(s)
      Asano T, Mori T. et al.
    • Journal Title

      Curr Drug Targets- CNS & Neurologic Disord (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Arundic acid (ONO-2506) ameliorates ischemic brain damage by preventing astrocytic overproduction of S100B.2005

    • Author(s)
      Asano T et al.
    • Journal Title

      Current Drug Targets-CNS & Neurologic Disorder (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Attenuation of a delayed increase in the extracellular glutamate level in the peri-infarct area following focal cerebral ischemia by a novel agent ONO-2506.2004

    • Author(s)
      Mori T., Asano T
    • Journal Title

      Neurochem Int 45(2-3)

      Pages: 381-387

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Attenuation of a delayed increase in the extracellular glutamate level in the peri-infarct area following focal cerebral ischemia by a novel agent ONO-25062004

    • Author(s)
      Mori T, Asano T
    • Journal Title

      Neurochem Int 45(2-3)

      Pages: 381-387

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Astrocytic activation and delayed infarct expansion fdlowing permanent focal ischemia in rats. Part I : Enhanced astrocytic synthesis of S-100β in the periinfarct area precedes the occurrence of delayed infarct expansion.2002

    • Author(s)
      Matsui T., Mori T., Morikawa E., Asano T
    • Journal Title

      J Cereb Blood Flow and Metab 22(6)

      Pages: 711-722

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Astrocytic activation and delayed infarct expansion following permanent focal ischemia in rats. Part II : Suppression of astrocytic activation by a novel agent (R)-(-)-2 propyloctanoic acid (ONO-2506) leads to mitigation of delayed infarct expansion and early improvement of neurological deficits.2002

    • Author(s)
      Tateishi N, Mori T, Morikawa E, Asano T
    • Journal Title

      J Cereb Blood Flow and Metab 22(6)

      Pages: 723-734

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Astrocytic activation and delayed infarct expansion following permanent focal ischemia in rats. Part I : Enhanced astrocytic synthesis of S- 100β in the periinfarct area precedes the occurrence of delayed infarct expansion2002

    • Author(s)
      Matsui T, Mori T, Morikawa E, Asano T
    • Journal Title

      J Cereb Blood Flow and Metab 22(6)

      Pages: 711-722

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Astrocytic activation and delayed infarct expansion following permanent focal ischemia in rats. Part II : Suppression of astrocytic activation by a novel agent (R)-(-)-2 propyloctanoic acid (ONO-2506) leads to mitigation of delayed infarct expansion and early improvement of neurological deficits2002

    • Author(s)
      Tateishi N, Mori T, Morikawa E, Asano T
    • Journal Title

      J Cereb Blood Flow and Metab 22(6)

      Pages: 723-734

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] The role of glia in neurotoxicity(Aschner M and Costa LG ed.)2005

    • Author(s)
      Asano T., Mori T., et al.
    • Publisher
      CRC press L.L.C., (Boca Raton)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Role of astrocytes in medication of stroke : Possible detrimental role of astrocytic activation during the subacute phase of permanent focal cerebral ischemia in the rat (The role of glia in neurotoxicity) (Aschner M and Costa LG ed.)2005

    • Author(s)
      Asano T, Mori T
    • Publisher
      CRC press L.L.C., (Boca Raton)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] The role of glia in neurotoxicity(Aschner M and Costa LG ed.)2005

    • Author(s)
      Asano T et al.
    • Publisher
      CRC press L.L.C., (Boca Raton)
    • Related Report
      2004 Annual Research Report
  • [Book] Molecular mechanism and epochal therapeutics of ischemic stroke and dementia(Abe K ed.)2003

    • Author(s)
      Asano T., Mori T., et al.
    • Publisher
      Elesvier Science Publishers B.V., (Amsterdam)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Functional modulation of astrocytes by a novel agent ONO-2506 mitigates delayed infarct expansion with a wide therapeutic time window, inducing prompt neurological recovery through reduction of the extracellular level of glutamate (Molecular mechanism and epochal therapeutics of ischemic stroke and dementia) (Abe K ed.)2003

    • Author(s)
      Asano T, Mori T et al.
    • Publisher
      Elsevier Science Publishers B.V., (Amsterdam)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Molecular mechanism and epochal therapeutics of ischemic stroke and dementia(Abe K ed.)2003

    • Author(s)
      Asano T et al.
    • Publisher
      Elsevier Science Publishers B.V., (Amsterdam)
    • Related Report
      2004 Annual Research Report
  • [Publications] Narito Tateishi, Takashi Mori,......Eiharu Morikawa,....Takao Asano: "Astrocytic activation and delayed infarct expansion following permanent focal ischemia in rats. Part II : Suppression of astrocytic activation by a novel agent (R)-(-)-2-propyloctanoic acid (ONO-2506)leads to mitigation of delayed infarct expansion and early improvement of neurological deficits."Journal of Cerebral blood Flow & Metabolism. 22. 723-743 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takao Asano,...Takashi Mori,..(分担): ""Molecular mechanism and epochal therapeutics of ischemic stroke and dementia""Abe K ed.. 147-151 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Toru Matsui, Takashi Mori, Eiharu Monikawa, Takao Asano: "Astrocytic activation and delayed infarct expansion following permanent focal ischemia in rats. Part I : Enhanced astrocytic synthesis of S-100β in the periinfarct area precedes the occurrence of delayed infarct expansion"Journal of Cerebral blood Flow & Metabolism. 22. 711-722 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 浅野 孝雄: "脳血管障害-急性期治療から予防まで-(分担):脳虚血の分子生物学の進歩"MEDICAL VIEW 社. 23-36 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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