| Project/Area Number |
14571334
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Teikyo University |
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Principal Investigator |
MATSUNO Akira Teikyo University, School of Medicine, Professor, 医学部, 助教授 (00242058)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Hideki Teikyo University, School of Medicine, Staff Assistant, 医学部, 助手 (50276713)
KATAYAMA Haruko Teikyo University, School of Medicine, Staff Assistant, 医学部, 助手 (40307187)
NAGASHIMA Tadashi Teikyo University, School of Medicine, Professor, 医学部, 教授 (70217991)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Pituitary hormone / secretion / rab3B / SNAP-25 / syntaxin / GFP / antisense / アンチセンス |
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| Research Abstract |
We have elucidated the role of rab3B in growth hormone (Gil) secretion and the mutual relationship between rab3B and soluble N-ethylrnaleimide-sensitive factor attachment protein receptor (SNARE) proteins. Confocal laser scanning microscopic observation of double immunohistochemical stainings for rab3B and GH revealed an increase of imrnunoreactivity for rab3B in GHRH-treated rats and a decrease of immunoreactivity for rab3B in SRLF-treated rats. Moreover, confocal laser scanning microscopic observation of double immunohistochemical stainings of SNAP-25, syntaxin and rab3B revealed co-localization of rab3B and the SNARE proteins in GI-IRH-treated rats, and dissociation in SRIF-treated rats. These results suggest that rab3B plays an important role in. Gil secretion from the anterior pituitary cells, and that SNAP-25 and syntaxin act alongside rab3B in the functional regulation of GH secretion. Another experiment using green fluorescent protein(GFP) has revealed the role of rab3B in the early stage of secretion of GH and the role of SNARE protein in the secretion of GH. The suppresion of rab3B is shown to inhibit the secretion of GH.
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