| Project/Area Number |
14571339
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
TAKAHASHI Hiroshi Nippon Medical School, School of Medicine, Professor, 医学部, 教授 (30150741)
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| Project Period (FY) |
2002 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | DNA microarray / interferon / anti-tumor effect / signal transmitter / malignant glioma / RT-PCR / clustering |
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| Research Abstract |
Comprehensive gene analysis of signal transmitters for anti-tumor effect of interferon using DNA microarray was performed. mRNA was extracted from surgical specimens of malignant gliomas. As control sample, mRNA was also extracted from many numbers of normal human brain tissues. Results of this gene analysis were listed below.
1)Significant changes (p<0.05) were recognized by 11 genes when having compared grade 4r with grade2.
2)Results of clustering analysis about selected genes showed 3 different expression patterns, and each expression pattern was strongly related with median survival time of the patients.
3)Two dimension re-clustering analysis about minimally selected 6 genes showed above-mentioned same 3 different expression patterns. Therefore, it was thought that those 6 selected genes might be prognostic markers of malignant glioma.
4)There were good correlation between microarray analysis value and RT-PCR analysis value about minimally selected 5 genes.
5)It is needed that the number of malignant glioma cases by cDNA microarray expression analysis will be increased. However, the results of this study suggested the tailor-made treatment against malignant glioma in future.
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