| Project/Area Number |
14571366
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | KANAZAWA UNIVERSITY |
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Principal Investigator |
KAWAHARA Norio Kanazawa Univ., Graduate School of Medicine, Associate Prof., 医学部付属病院, 助教授 (70214674)
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| Co-Investigator(Kenkyū-buntansha) |
TOMITA Katsuro Kanazawa Univ., Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (00092792)
MURAKAMI Hideki Kanazawa Univ., Graduate School of Medicine, 医学部付属病院, 助手 (70334779)
AKAMARU Tomoyuki Kanazawa Univ., Graduate School of Medicine, 大学院・医学系研究科, 助手 (50332664)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | bone morphogenetic protein / spinal tumor / spinal fusion / 脊椎固定 |
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| Research Abstract |
20 Dogs underwent total spondylectomy of TI3 and spinal reconstruction with titanium mesh cage using autogeneous bone graft(resected vertebral body) and recombinant human bone morphogenetic protein-2(rhBMP-2) to evaluate bone remodeling and fusion.
Control group(autogeneous bone graft n=10):had the titanium cage reconstruction filled with autogenous bone, and BMP-treated group(n=10):had reconstruction with the titanium cage filled collagen sponge soaked with rhBMP-2. 5 dogs of each group were killed 8weeks(group A) and 16 weeks(group B) after the surgery and we evaluated bone remodeling and fusion by imaging test(Xp, CT scan) and histologic examination.
Imaging test revealed bone fusion in all specimens with the exception of one pseudoarthrosis in the control group. Histologic examination showed 4 of 5 dogs in control group A had normal lamella of trabecular bone formation from the endplate of the adjacent vertebral bodies towards the center of the cage, and woven bone was present around the center of cage. It is considered to be a progress of remodeling. The other one dog had pseudoarthrosis. 3 of 5 dogs in control group B achieved consecutive trabecular cancellous bony fusion between adjacent vertebral bodies. The other two had more progressive abone formation towards the center of the mesh compared to the control group A. It is considered that bone fusion is going to be progressed.
In BMP-treated group A, there were progressive bone formation towards the center of the mesh. All the 5 dogs in BMP-treated group B achieved consecutive trabecular cancellous bony fusion between the grafted bone and adjacent vertebral bodies.
Both groups could have favorable bone fusion, but BMP-treated group had more trabecular bone formation throughout the length of the mesh than control group.
This study confirms rhBMP2 augment bony fusion and remodeling on vertebral reconstruction after total spondylectomy.
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