| Project/Area Number |
14571395
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Teikyo University (2003)
Kyoto Prefectural University of Medicine (2002) |
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Principal Investigator |
TAKEI Shinro Teikyo University, Medicine, Professor, 医学部, 教授 (10226730)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | articular cartilage / osteochondral defect / tissue regeneration / tidemark / mesenchymal cell / autologous cell transplantation / transgenic rat / in situ hybridization / 軟骨石灰化前線 |
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| Research Abstract |
The research which used the autologous cell graft for joint cartilage defect restoration has accomplished mostly. Restoration of normal hyaline cartilage has not been attained. We have completed the method for distinguishing donor and recipient cells using the combination of transgenic rat and DNA in situ hybridization. We reported those in the 48th and 49th Annual Meeting of the Orthopaedic Research Society (ORSI US), and the 18th Japanese Orthopaedic Association Basic Scientific Meeting, 2002, and 2003 USA, 2002, 2003. Using this technique, it was clearly demonstrated that cells from surrounding bone marrow replace the underlining bone of the donor with the donor cells. The model of following the transplanted cells was completed using GFP transgenic rats. This method is more reliable than the previously reported method using transgenic rat and DNA in situ hybridization. The results were reported in the 50th Annual Meeting of the Orthopaedic Research Society (ORS, US).
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