| Project/Area Number |
14571410
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
MATSUSHITA Kazuhiko St.Marianna University, School of Medicine, Associate Professor, 医学部, 助教授 (50190450)
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| Co-Investigator(Kenkyū-buntansha) |
ICHIMAN Yoshitoshi Tsukuba College of Technology, Dept. of Hygiene and Public Health, Professor, 衛生・公衆衛生学, 教授 (70090115)
AOKI Haruhito St.Marianna University School of Medicine, Dept. of Orthopaedic Surgery, Professor, 医学部, 教授 (40096516)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | artificial material / Staphylococcus aureus / bacterial adherence / antibody / inhibition |
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| Research Abstract |
The purpose of this study was to examine whether coating the surface of artificial material by human serum antibody against encapsulated strain of Staphylococus aureus (S.aureus) inhibit bacterial adherence to the surface
For S. aureus strain, the SMU-01 strain of methicillin-resistant S. aureus was used. The sera used were obtained from a total of 48 individuals, comprised of 28 healthy volunteers and 20 diabetic patients. A specific and rapid enzyme-linked immunosorbent assay (ELISA) inhibition test was employed for detection of immunoglobulins to S. aureus capsular polysaccharide in human serum. Two sera from these 48 subjects, with higher antibody titers to Smith strain (IgM 18%, IgG 57%, IgA 1%), and with lower antibody titers to Smith Strain (IgM 0.5%. IgG 0%, IgA 0%) were used in this study. Serum was diluted 100-fold and 1000-fold. Adherence to plastic surface of this strain was measured by the method of Mackenzie et al.
In the 1000-fold higher antibody titers serum group, average percent Index of adherence (IA%) was 26.9%. In the 1000-fold lower antibody titers serum group, average IA% was 31.7%. In the 100-fold higher antibody titers serum group, average IA% was 19.7%. In the 1000-fold lower antibody titers serum group, average percent IA was 25.3%.
There was tendency that bacterial adherence to the plastic surface was inhibited by human serum with higher antibody titers to Smith strain. Also this action depended on the amount of antibody present.
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