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Management of artificial respiration worsens acute lung injury.-Investigation and influence of mechanism by molecular biological approach-

Research Project

Project/Area Number 14571434
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionKobe University

Principal Investigator

MORIKAWA Osamu  Kobe University Hospital, Associate Professor, 医学部附属病院, 助手 (50335436)

Co-Investigator(Kenkyū-buntansha) NISHIMA Kahoru  Kobe University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 講師 (20311780)
MIKAWA Katsuya  Kobe University, Graduate School of Medicine, Assistant Professor, 大学院・医学系研究科, 助教授 (40229662)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsalveolar type II cell / cell stretch
Research Abstract

Alveolar epithelial cells type II (AEC-II) are thought to play an important role in the pathogenesis of acute lung injury because AEC-II can produce many types of pro-inflammatory cytokines and chemokines. Especially, Interleukin-8 (IL-8) plays an important role in this inflammatory response. AEC-II are exposeed to mechanical stretch during breathing and mechanical ventilation. Increased stretch may contribute to lung injury. The purpose of this study is to elucidate the effects of cell stretch on endotoxin-induced interleukin-8 (IL-8) production from cultured rat AEC-II.
After isolation of rat AEC-II, cells were cultured for 48h. LPS (lipopolysaccaraide) increased rat GRO/CINC (IL-8) measured by ELISA in rat alveolar type II cell cultured media. Cells were stretched using mechanical stretch machine (Flexcell). At 3h, 6h, 12h and 24h, the cell media were collected and GRO/CINC (IL-8) were measured by ELISA. The concentration of GRO/CINC (IL-8) increased time-dependently in 24h. LPS & cell Stretch induced additional increase compared with each study.
These results showed cell damage induced by cell stretch was similar to ventilator-induced lung injury and LPS & stretch means mechanical ventilation in septic state in clinical. This model may help for future therapy and mechanizm for acute lung injury induced by sepsis.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 森川 修: "急性肺傷害の病態と治療"臨床麻酔. 26(増刊号). 339-353 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 三川 勝也: "ALI/ARDS治療の最新の進歩:サーファクタント補充療法"現代医療. 34(増刊号). 2059-2067 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Osamu, Morikawa: "The mechanism and treatments of acute lung injury."Journal of Clinical Anesthesia. 26. 339-253 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Katsuya, Mikawa: "Surfactant replacement therapy for ALI/ARDS."New Horizon for medicine. 34. 2059-2067 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 森川 修: "急性肺傷害の病態と治療"臨床麻酔. 26(増刊号). 339-353 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 三川 勝也: "ALI/ARDS治療の最新の進歩:サーファクタント補充療法"現代医療. 34(増刊号). 2059-2067 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 森川 修: "急性肺傷害の病態と治療"臨床麻酔. 26(増刊号). 339-353 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 三川 勝也: "ALI/ARDS治療の最新の進歩:サーファクタント補充療法"現代医療. 34(増刊号). 2059-2067 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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