| Project/Area Number |
14571508
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Oita University (2004)
大分医科大学 (2002-2003) |
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Principal Investigator |
MIMATA Hiromitsu Oita University, Faculty of Medicine, PROFESSOR, 医学部, 教授 (60219714)
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| Project Period (FY) |
2002 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | stress incontinence / urethral rhabdosphincter / skeletal muscle / growth factor / 尿失禁 / シグナル伝達 / HGF / IGF-I / cDNA microarray / bFGF / サイトカイン / ストレス応答 / MAPキナーゼ / PI3キナーゼ |
|---|
| Research Abstract |
Regeneration of urethral rhabdosphincter muscle will be a new possible therapy for urinary stress incontinence. We selectively cultured skeletal satellite cells from human urethral rhabdosphincter using neural cell adhesion molecule as a cell surface marker. Proliferation of satellite cells of urethral rhabdosphincter is enhanced by growth factors such as hepatocyte growth factor(HGF) and insulin-like growth factor-I(IGF-I) via MAPK and PI3-K signaling pathways, respectively. As the satellite cells produced both HGF and IGF-I and the neutralizing antibodies against HGF and IGF-I inhibited the growth, these growth factors stimulate the growth of the urethral rhabdosphincter cells as autocrine frashion. These findings suggest that local injections of HGF and IGF-I around membranous urethra may be useful to regenerate urethral rhabdosphincter to treat stress incontinence.
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