| Project/Area Number |
14571535
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Akita University |
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Principal Investigator |
FUKUDA Jun Akita university, School of Medicine, Lecture, 医学部, 講師 (80250877)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAMURA Kazuhiro Akita university, School of Medicine, Research Associate, 医学部, 助手 (10344756)
TANAKA Toshinobu Akita university, School of Medicine, Professor, 医学部, 教授 (40002216)
KUMAGAI Jin Akita university, School of Medicine, Research Staff, 医学部, 医員(臨床) (60333936)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Survivin / Caspase / Apoptosis / Embryo / ラット / アンチセンス |
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| Research Abstract |
The antisense approach was used to demonstrate the role of survivin on embryo development. Development of early embryos treated with antisense survivin oligonucleotides was arrested at the molura or early blastocyst stage with disruption of tubulin formation and abnormal nuclei, associated with apoptosis. The effect of the antisense was enhanced by cotreatment with an apoptosis-inducing reagent, staurosporine. In contrast, apoptosis induced by the antisense tratment was inhibited by caspase-3 and caspase-9 inhibitors. These results, indicate that survivin is an essential antiapoptotic gene expressed in preimplantation embryos and could protect the embryos from apoptosis by inducing an apoptotic pathway involving caspases.
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