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Analysis of molecular regulation on female reproductive tract during fertilization using genetic model mice

Research Project

Project/Area Number 14571537
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionYamagata University

Principal Investigator

ARAKI Yoshihiko  Yamagata University, School of Medicine, Associate Professor, 医学部, 助教授 (70250933)

Co-Investigator(Kenkyū-buntansha) SHINKAI Yoichi  Kyoto University, Institute for Virus Research, ウイルス研究所, 教授 (20211972)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsfemale reproductive tract / fertilization / oviduct-specific glycoprotein / transgenic mouse / knock-out mouse / エストロゲン / プロモーター
Research Abstract

The mammalian fertilization process takes place in a complex microenvironment within the female genital tract. A member of the chitinase protein family, oviduct-species glycoprotein (OGP), has been identified in oviductal fluid from various mammalian species including humans. Although it is thought that OGP is widely believed to involve in the process of mammalian fertilization, including sperm function and gamete interactions based on experimental results obtained in vitro, its physiological significance remains controversial. In this study, we generated transgenic mice using a 2.2-kilobase (kb) segment of the 5'-flanking sequence of the OGP gene for expression of the SV 40 T antigen (Tag). These mice spontaneously developed tumors in the female reproductive tract. Analysis using RT-PCR showed that the 2.2-kb OGP 5'-flanking region drove Tag mRNA expression in the oviduct, uterus, vagina and ovary, but not in other tissues. Ovariectomy suppressed Tag expression and thereby blocked tum … More origenesis in the transgenic mice. Estradiol administration to ovariectomized transgenic mice led to dramatic hyperplasia of the reproductive tract tissues in association with enhanced Tag expression, both in intensity and distribution. These results demonstrated that a 2.2-kb fragment of the 5'-flanking sequence of the mouse OGP gene was capable of directing the expression of Tag and inducing tumorigenesis in female reproductive tract tissues in an estrogen-dependent the expression of Tag and inducing tumorigenesis in female reproductive tract tissues in an estrogen-dependent manner. Estrogen response elements present in the promoter region were functional in vivo. In In addition, we eatablished OGP gene-null (ogp^<-/-> mice, and primarily characterized their reproductive properties to study the physiological function(s) of OGP. Data obtained from studies using in vivo or in vitro system showed that the fertility of ogp^<-/-> females was within normal limits. These results indicate that OGP is dispensable for the process of in vivo fertilization, at least in mice Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Miyoshi et al.: "A mouse transgenic for murine oviduct-specific glycoprotein promoter-driven simian virus 40 large T-antigen : Tumor formation and its hormonal regulation."Mol.Reprod.Dev.. 63. 168-176 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimozawa et al.: "Abnormalities in cloned mice are not transmitted to the progeny."Genesis. 34. 203-207 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Araki et al.: "Immortalized epididymal cell lines from transgenic mice over expressing temperature-sensitive siman virus 40 T-antigen gene."J.Androl.. 23. 23:854-23:869 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki et al.: "The 5'-flanking region of the murine epididymal protein of 17 kilodalton gene targets transgene expression in the epididymis."Endocrinology. 144. 877-886 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeda et al.: "Expression of GPI-80, a β2 integrin-associated glycosylphosphatidyl inositol anchored protein, requires neutrophil differentiation with dimethylsulfoxide in HL-60 cells."Exp.Cell Res.. 286. 199-208 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Araki et al.: "Effect of a null mutation in the oviduct-specific glycoprotein gene on mouse fertilization."Biochem.J.. 374. 551-557 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Araki Y.: "Formation and structure of mammalian ovaries. In : Introduction to Mammalian Reproduction (Tulsiani DRP ed.) P141-153."Kluwer Academic Publishers, Norwell, MA, USA.. 403 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyoshi et al.: "A mouse transgenic for murine oviduct-specific glycoprotein promoter-driven simian virus 40 large T-antigen: Tumor formation and its hormonal regulation."Mol.Reprod.Dev.. 63. 168-176 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimozawa et al.: "Abnormalities in cloned mice are not transmitted to the progeny."Genesis. 34. 203-207 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Araki et al.: "Immortalized epididymal cell lines from transgenic mice over expressing temperature-sensitive siman virus 40 T-antigen gene."J.Androl.. 23. 854-869 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki et al.: "The 5'-flnaking region of the murine epididymal protein of 17 kilodalton gene targets transgene expression in the epididymis."Endocrinology. 144. 877-886 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeda et al.: "Expression of GPI-80, a β integrin-associated glycosylphosphatidyl inositol anchored protein, requires neutrophil differentiation with dimethylsulfoxide in HL-60 cells"Exp.Cell Res.. 286. 199-208 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Araki et al.: "Effect of a null mutation in the oviduct-specific glycoprotein gene on mouse fertilization."Biochem.J.. 374. 551-557 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyoshi et al.: "A mouse transgenic for murine oviduct-specific glycoprotein promoter-driven simian virus 40 large T-antigen : Tumor formation and its hormonal regulation"Mol.Reprod.Dev.. 63. 168-176 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shimozawa et al.: "Abnormalities in cloned mice are not transmitted to the progeny"Genesis. 34. 203-207 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Araki et al.: "Immortalized epididymal cell lines from transgenic mice over expressing temperature-sensitive siman virus 40 T-antigen gene"J.Androl.. 23. 23:854-23:869 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki et al.: "The 5'-flanking region of the murine epididymal protein of 17 kilodalton gene targets transgene expression in the epididymis"Endocrinology. 144. 877-886 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takeda et al.: "Expression of GPI-80, a β2 integrin-associated glycosylphosphatidyl inositol anchored protein, requires neutrophil differentiation with dimethylsulfoxide in HL-60 cells"Exp.Cell Res.. 286. 199-208 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Araki et al.: "Effect of a null mutation in the oviduct-specific glycoprotein gene on mouse fertilization"Biochem.J.. 374. 551-557 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Araki Y.: "Formation and structure of mammalian ovaries. In : Introduction to Mammalian Reproduction(Tulsiani DRP ed.) P141-153"Kluwer Academic Publishers, Norwell, MA, USA. 403 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyoshi et al.: "A mouse transgenic for murine oviduct-specific glycoprotein promoter-driven simian virus 40 large T-antigen : Tumor formation and its hormonal regulation"Molecular Reproduction and Devevelopment. 63. 168-176 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nitto et al.: "Phamacological analysis for mechanisms of GPI-80 release from tumor necrosis factor-α-stimulated human neutrophils"British Journal of Pharmacology. 137. 353-360 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimozawa et al.: "Abnormalities in cloned mice are not transmitted to the progeny"Genesis. 34. 203-207 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Araki et al.: "Immortalized epididymal cell lines from transgenic mice over expressing temperature-sensitive siman virus 40 T-antigen gene"Journal of Andorology. 23. 854-869 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki et al.: "The 5'-flanking region of the murine epididymal protein of 17 kilodalton gene targets transgene expression in the epididymis"Endocrinology. 144. 877-886 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takeda et al.: "Expression of GPI-80,a β2 integrin-associated glycosylphosphatidyl inositol anchored protein, requires neutrophil differentiation with dimethylsulfoxide in HL-60 cells"Experimental Cell Research. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Araki Y.: "Formation and structure of mammalian ovaries. In : Introduction to Mammalian Reproduction (Tulsiani DRP ed.) P141-153"Kluwer Academic Publishers, Norwell, MA, USA.. 403 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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