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Effects of vasoactive substances on ovarian function and implantation in the molecular biological approach

Research Project

Project/Area Number 14571544
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KUBOTA Toshiro  Tokyo Medical & Dental Univ., Comprehensive Reproductive Medicine, Associate Professor, 大学院・医歯学総合研究科, 助教授 (50126223)

Co-Investigator(Kenkyū-buntansha) ASO Takeshi  Tokyo Medical & Dental Univ., Comprehensive Reproductive Medicine, Professor, 大学院・医歯学総合研究科, 教授 (60093176)
己斐 秀樹  東京医科歯科大学, 大学院・医歯学総合研究科, 助手 (20280969)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsnitric oxide (NO) / NO synthase (NOS) / Carbon monoxide (CO) / Heme oxygenase (HO) / luteinal function / implantation / human endometrium / granulosa cells / Heme oxenase(HO) / 一酸化炭素 / 妊娠初期絨毛 / ブタ顆粒膜細胞 / アポトーシス / Fas ligand / Heme oxygenase / 卵巣機能 / ブタ卵巣 / 性ステロイド産生 / Heme oxygenase(HO) / 妊娠初期ヒト絨毛 / 黄体期
Research Abstract

The aim of the present study was undertaken to investigate some vasoactive substances derived from vascular endotherial cells, namely the nitric oxide(NO)/NO synthase(NOS) system and the carbon monoxide (CO)/heme oxygenase (HO) system, on the differentiation induced by sex steroid hormones in human endometrial cells of the secretory phase and human decidual cells in implantation, and on the follicular development or luteinization in porcine granulose cells by mainly using molecular biological methods. These studies reveal that NO locally synthesized by iNOS could be involved in the control of endometrial functions, and NO/NOS system play an important role in the maintenance of pregnancy. Furthermore, it is suggested that the CO/HO system possibly play a role in the local control of endometrial function in normal menstrual periods. CO/HO may also be involved in the control of placental vascular function, which protect the syncytiotrophoblast and endothelium against oxidative injury. In ovarian functions, these results demonstrate that NO inhibits E2 secretion independent of cGMP by inhibiting P450 aromatase activity in moderately mature PGC, and strongly suggest that the NO/NOS system in PGC regulates steroidogenesis differently during different phase of follicular development. On the other hand, we conclude that products of reactions catalyzed by CO/HO system are likely to be important autocrine/paracrine factors that regulate apoptosis in PGC.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (31 results)

All 2004 2003 2002 Other

All Journal Article (16 results) Book (2 results) Publications (13 results)

  • [Journal Article] Haem oxygenase augments porcine granulosa cell apoptosis in vitro2004

    • Author(s)
      Harada T, Koi H, Kubota T, Aso T
    • Journal Title

      Journal of Endocrinology 181

      Pages: 191-205

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Estrogen and progesterone improve scopolamine-induced impairment of spatial memory2004

    • Author(s)
      Tanabe F, Miyasaka N, Kubota T, Aso T
    • Journal Title

      Journal of Medical and Dental Science 51

      Pages: 89-98

    • NAID

      110000076056

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Three prostaglandin E_2 synthases on mice premature delivery2004

    • Author(s)
      Kubota K, Kubota T, Morita I, Aso T
    • Journal Title

      86^<th> Annual Meeting of the Endochine Society (Abstracts)

      Pages: 346-346

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Estrogen and progesterone improve scopalamine-induced impairment of spatial memory.2004

    • Author(s)
      Tanabe F, Miyasaka N, Kubota T, Aso T
    • Journal Title

      Journal of Medical and Dental Science 51

      Pages: 89-98

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Haem oxygenase augments porcine granulosa cell apoptosis in vitro.2004

    • Author(s)
      Harada T, Koi H, Kubota T, Aso T
    • Journal Title

      Journal of Endocrinology 181

      Pages: 191-205

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Androgenic effects on adrenocortical responsiveness in neonatal rats, In vivo2004

    • Author(s)
      Yoshimura S, Kubota T, Suzuki S, Sassa S, Kudi H, Sakamoto S
    • Journal Title

      In vivo 18

      Pages: 697-702

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Heme oxygenase augments porcine granulosa cell apoptosis in vitro.2004

    • Author(s)
      Harada T, Koi H, Kubota T, Aso T
    • Journal Title

      The International Ovarian Conference 2004 Abstracts

      Pages: 39-39

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Three prostaglandin E2 synthases on mice premature delivery.2004

    • Author(s)
      Kubota K, Kubota T, Morita I, Aso T
    • Journal Title

      86^<th> Annual Meeting of the Endocrine Society Abstracts

    • Related Report
      2004 Annual Research Report
  • [Journal Article] マウス切迫早産における膜結合型および細胞質型PGE2 合成酵素の発現2004

    • Author(s)
      久保田健, 久保田俊郎 他
    • Journal Title

      第56回日本産科婦人科学会学術講演会 Abstracts

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 一酸化窒素/一酸化炭素の着床への関与2003

    • Author(s)
      久保田 俊郎
    • Journal Title

      子宮内膜疾患Proceedings

      Pages: 8-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Heme oxygenase augments porcine granulosa cell2003

    • Author(s)
      Harada T, Koi H, Kubota T, Aso T
    • Journal Title

      85^<th> Annual Meeting of the Endochine Society (Abstracts)

      Pages: 480-480

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ブタ卵巣におけるHeme oxygenaseの発現とapoptosisに与える影響2003

    • Author(s)
      原田竜也, 己斐秀樹, 久保田俊郎 他
    • Journal Title

      第55回日本産科婦人科学会学術講演会 (Abstracts)

      Pages: 106-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Heme oxygenase augments porcine granulosa cell.2003

    • Author(s)
      Harada T, Koi H, Kubota T, Aso T
    • Journal Title

      85^<th> Annual Meeting of the Endocrine Society, #P3-24, Philadelphia PA, USA, June

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Usefulness of CA199 versus CA125 for the diagnosis of endometriosis.2002

    • Author(s)
      Harada T, Kubota T, Aso T
    • Journal Title

      Fertility & Sterility 78(4)

      Pages: 733-739

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Lymphadenectomy and adjuvant therapy in endometrial carcinoma : role of adjuvant chemotherapy.2002

    • Author(s)
      Otsuka I, Kubota T, Aso T
    • Journal Title

      British Journal of Cancer 87

      Pages: 377-380

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Change in prostaglandin E synthases on mPGES-1 gene-disrupted mice in preterm

    • Author(s)
      Kubota K, Kubota T, Murakami M, Kudo I, Aso T, Moria I
    • Journal Title

      Journal of Endocrinology (accepted.)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 内科学レビュー2004

    • Author(s)
      久保田俊郎, 麻生武志
    • Total Pages
      349
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 内科学レビュー2004

    • Author(s)
      久保田俊郎, 麻生武志
    • Total Pages
      349
    • Related Report
      2004 Annual Research Report
  • [Publications] Harada T, Koi H, Kubota T et al.: "Heme oxygenase augments porcine gnanulosa cell"85th, Annual Meeting of the Endocrine Society Abstracts p480 philadelphia USA 2003. 480 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 久保田 俊郎: "一酸化窒素/一酸化炭素の着床への関与"子宮内膜疾患proceedings. 8-12 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 吉木尚之, 久保田俊郎: "目で見る着床過程、血管作動性生理活性物質の役割"Hormone Frontier in Gynecology. 10(3). 222-225 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 久保田俊郎: "内分泌検査 2.性腺機能検査"検査と技術. 31(5). 435-439 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 原田竜也, 己斐秀樹, 久保田俊郎他: "ブタ卵巣におけるHene Oxygenaseの発現とapoptosisに与える影響"日本産科婦人科学会雑誌. 55(2). 206 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 原田竜也, 己斐秀樹, 久保田俊郎他: "ブタ卵巣顆粒膜細胞におけるHene Oxygenaseのapoptosisに与える影響"日本不妊学会雑誌. 48(3,4). 304 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 久保田 俊郎: "リスクマネージメントの実際 産婦人科領域"医薬ジャーナル社. 335 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Harada T, Kubota T et al.: "Usefulness of CA19-9 Versus CA125 for the diagnosis of endometriusis"Fertility and Sterility. 78(4). 733-739 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 久保田 俊郎: "機能性出血の定義とその分類"産科と婦人科. 69(4). 409-414 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 原田竜也, 己斐秀樹, 久保田俊郎他: "ヒト卵巣におけるHeme oxynegaseの発現に関する検討"日本不妊学会雑誌. 47(4). 147 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 原田竜也, 己斐秀樹, 久保田俊郎他: "ブタ卵巣におけるHOの発現とステロイド産生能に与える影響に関する検討"日本内分泌学会雑誌. 78(2). 394 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 久保田俊郎: "生殖内分泌「産婦人科におけるEBMを考える」"日本産科婦人科学会関連連合地方部会会報. 39(2). 103 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 久保田 俊郎: "今日の治療指針 第5版"医学書院. 2092 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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