| Co-Investigator(Kenkyū-buntansha) |
KOTSUJI Fumikazu University of Fukui, Faculty of Medical Sciences, Professor, 医学部, 教授 (50153573)
KAWAHARA Kazumi University of Fukui, Faculty of Medical Sciences, Research Associate, 医学部附属病院, 助手 (60234100)
KUROKAWA Tetsuji University of Fukui, Faculty of Medical Sciences, Research Associate, 医学部, 助手 (60334835)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Research Abstract |
Metastatic consists of multiple steps including invasion, transportation and proliferation in target organs.
Metastasis associated molecules, like extracellular matrix proteinases and their inhibitors have been identified for some time, but we are still far from understanding the complex mechanisms operating in metastasis.
Differentially expressed genes from different specimens may be detected with parallel analysis by gene chips. This technique possesses many advantages, of which the greatest is to improve on traditional experiments by allowing a single or several gene expression differences to be observed in a single test. More cDNA microarray methods are being applied in the study of gene expression. In this paper, a gene chip technique was used to analyse the different gene expression patterns in the highly potential metastatic human ovarian cancer cell line, ykt-m, and its mother cell line, ykt, as well as in low potential metastatic human ovarian cancer cell line.
A total of 2 genes, which were EGR1 and placental calcium-binding protein(calvasculin), with expression levels significantly larger were found by comparing the ykt, which had low potential metastasis. A total of 8 genes, which were retinoic acid receptor beta, BRCA1-associated ring domain protein, integrin beta8precursor, fibroblast growth factor, RBQ1retinoblastoma binding protein, HLA-DRantigen-associated invariant subunit, MHC class II HLA-DR-beta precursor, and cleavage stimulation factor 77-kDa subunit, with expression levels were significantly lower were found.
cDNA microarray techniques are effective in screening differential gene express ion between two human ovarian cancer cell lines (ykt-m ; ykt).These genes may be related to the genesis and metastasis of ovarian carcinoma.
Analysis of the human ovarian cancer gene express ion profile with cDNA microarray may help in gene diagnosis, treatment and prevention.
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