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Cloning of differentially expressed genes in highly and low metastatic human ovarian cancer by microarray

Research Project

Project/Area Number 14571549
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionFaculty of Medical Sciences, University of Fukui

Principal Investigator

YOSHIDA Yoshio  University of Fukui, Faculty of Medical Sciences, Assistant Professor, 医学部附属病院, 講師 (60220688)

Co-Investigator(Kenkyū-buntansha) KOTSUJI Fumikazu  University of Fukui, Faculty of Medical Sciences, Professor, 医学部, 教授 (50153573)
KAWAHARA Kazumi  University of Fukui, Faculty of Medical Sciences, Research Associate, 医学部附属病院, 助手 (60234100)
KUROKAWA Tetsuji  University of Fukui, Faculty of Medical Sciences, Research Associate, 医学部, 助手 (60334835)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsOvarian Cancer / Oligonucleotide / EGRI / Calvasculin / Metastasis
Research Abstract

Metastatic consists of multiple steps including invasion, transportation and proliferation in target organs.
Metastasis associated molecules, like extracellular matrix proteinases and their inhibitors have been identified for some time, but we are still far from understanding the complex mechanisms operating in metastasis.
Differentially expressed genes from different specimens may be detected with parallel analysis by gene chips. This technique possesses many advantages, of which the greatest is to improve on traditional experiments by allowing a single or several gene expression differences to be observed in a single test. More cDNA microarray methods are being applied in the study of gene expression. In this paper, a gene chip technique was used to analyse the different gene expression patterns in the highly potential metastatic human ovarian cancer cell line, ykt-m, and its mother cell line, ykt, as well as in low potential metastatic human ovarian cancer cell line.
A total of 2 genes, which were EGR1 and placental calcium-binding protein(calvasculin), with expression levels significantly larger were found by comparing the ykt, which had low potential metastasis. A total of 8 genes, which were retinoic acid receptor beta, BRCA1-associated ring domain protein, integrin beta8precursor, fibroblast growth factor, RBQ1retinoblastoma binding protein, HLA-DRantigen-associated invariant subunit, MHC class II HLA-DR-beta precursor, and cleavage stimulation factor 77-kDa subunit, with expression levels were significantly lower were found.
cDNA microarray techniques are effective in screening differential gene express ion between two human ovarian cancer cell lines (ykt-m ; ykt).These genes may be related to the genesis and metastasis of ovarian carcinoma.
Analysis of the human ovarian cancer gene express ion profile with cDNA microarray may help in gene diagnosis, treatment and prevention.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Tetsuji Kurokawa: "Expression of GLUT-1 glucose transfer, cellular proliferation activity and grade of tumor correlate with [F-18]-fluorodeoxyglucose uptake by positron emission tomography in epithelial tumors of the ovary."International Journal of Cancer. 109(6). 926-932 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshio Yoshida: "Incremental benefits of positron emission with [F-18] fluorodeoxiglucose over computed tomography alone for the preoperative staging of ovarian cancer."AJR.American Journal of Roentgenology. 182(1). 227-233 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yukiko Nishikawa: "Establishment of a novel human ovarian cancer cell line with high anchorage-independent growth ability."International Journal of Oncology. 23. 1679-1686 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshio Yoshida: "A Phase I Study of Continuous Adminidtration of 5-Fluorouracil/Cisplatin in Advanced Uterine Cervical Cancer."Anticancer Reseach. 22. 3473-3476 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tetsuji Kurokawa: "Whole-body PET with FDG is useful for following up an ovarian cancer patient with only rising CA125 levels within the normal range."Annals of Nuclear Medicine. 16(7). 491-493 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimihisa Tajima: "Establishment of follicle-stimulating hormone responsive cell lines by transfection of preovulatory human cells with mutated p53(p53val135)and Ha-ras genes."Molecular human reproduction. 8(1). 48-57 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tetsuji Kurokawa: "Expression of GLUT-1 glucose transfer, cellular proliferation activity and grade of tumor correlate with [F-18]-fluorodeoxyglucose uptake by positron emission tomography in epithelial tumors of the ovary."International Journal of Cancer. 109(6). 926-932 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshio Yoshida: "Incremental benefits of positron emission with [F-18] fluorodeoxiglucose over computed tomography alone for the preoperative staging of ovarian cancer."AJR. American Journal of Roentgenology. 182(1). 227-233 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yukiko Nishikawa: "Establishment of a novel human ovarian cancer cell line with high anchorage-independent growth ability."International Journal of Oncology. 23. 1679-1686 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshio Yoshida: "A Phase I Study of Continuous Adminidtration of 5-Fluorouracil/Cisplatin in Advanced Uterine Cervical Cancer."Anticancer Research. 22. 3473-3476 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tetsuji Kurokawa: "Whole-body PET with FDG is useful for following up an ovarian cancer patient with only rising CA125 levels within the normal range."Annals of Nuclear Medicine. 16(7). 491-493 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kimihisa Tajima: "Establishment of follicle-stimulating hormone responsive cell lines by transfection of preovulatory human cells with mutated p53(p53val135) and Ha-ras genes."Molecular human reproduction. 8(1). 48-57 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tetsuji Kurokawa: "Expression of GLUT-1 glucose transfer, cellular proliferation activity and grade of tumor correlate with[F-18]-fluorodeoxyglucose uptake by positron emission tomography in epithelial tumors of the ovary"International Journal of Cancer. 109(6). 926-932 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshio Yoshida: "Incremental benefits of positron emission with[F-18]fluorodeoxiglucose over computed tomography alone for the preoperative staging of ovarian cancer"AJR.American Journal of Roentgenology. 182(1). 227-233 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yukiko Nishikawa: "Establishment of a novel human ovarian cancer cell line with high anchorage-independent growth ability"International Journal of Oncology. 23. 1679-1686 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshio Yoshida: "A Phase I Study of Continuous Adminidtration of 5-Fluorouracil/Cisplatin in Advanced Uterine Cervical Cancer"Anticancer Reseach. 22. 3473-3476 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tetsuji Kurokawa: "Whole-body PET with FDG is useful for following up an ovarian cancer patient with only rising CA125 levels within normal range"Annals of Nuclear Medicine. 16(7). 491-493 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kimihisa Tajima: "Establishment of follicle-stimulating hormone responsive cell lines by transfection of preovulatory human cells with mutated p53(p53val135)and Ha-ras genes"Molecular human reproduction. 8(1). 48-57 (2002)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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